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Inhibition of human breast carcinoma by BLC (Sargassum fulvellum) and BLC/HEN Egg in vitro and in vivo

Jo, Eun-Hye    (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University   ); Cho, Sung-Dae    (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University   ); Ahn, Nam-Shik    (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University   ); Jung, Ji-Won    (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University   ); Yang, Se-Ran    (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University   ); Park, Joon-Suk    (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University   ); Hwang, Jae-Woong    (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University   ); Lee, Sung-Hoon    (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University   ); Park, Jung-Ran    (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University   ); Kim, Sun-Jung    (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University ;  ); Park, Hyun-Kyung   Lee, Yong-Soon   Kang, Kyung-Sun  
  • 초록

    Much of the interest on the chemopreventive properties of herbs and plants has been raised, whereas little is regarding to anti-tumor effect of farming and aquatic products. In the present study, the anti-tumor effect of hot-water extract of a seaweed, BLC (Sargassum fulvellum) and BLC/HEN egg was investigated using MCF-7 cells in vitro and in vivo systems. We found that the BLC extract and BLC/HEN egg inhibited cell proliferation in a dose-dependent manner, which might be mediated through up-regulation of p53. Furthermore, this test compound can directly induce apoptosis in MCF-7 cells, which might be mediated through up-regulation of a pro-apoptotic Bax protein and down-regulation of a anti-apoptotic Bcl-2 protein, not by immune system. Nude mice bearing established breast tumors (with exogenous estradiol) were treated with BLC extract and BLC/HEN egg. Treatment BLC extract and BLC/HEN egg caused a 42% and 71% inhibition of tumor growth, respectively. Both agents caused a significant inhibition of volume and weight growth of estrogen independent human breast tumors established from MCF-7 cells. Our results suggested that BLC extract and BLC/HEN egg have the efficacious effect of human breast cancer not only in vitro but also in vivo.


  • 주제어

    Sargassum fulvellum .   MCF-7 cells .   apoptosis .   Bcl-2 family .   xenograft.  

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