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Characteristics of Gene Structure of Bovine Ghrelin and Influence of Aging on Plasma Ghrelin

Kita, K.    (Laboratory of Animal Feeds and Production, University Farm, Graduate School of Bioagricultural Sciences Nagoya University   ); Harada, K.    (Laboratory of Animal Feeds and Production, University Farm, Graduate School of Bioagricultural Sciences Nagoya University   ); Nagao, K.    (Laboratory of Animal Feeds and Production, University Farm, Graduate School of Bioagricultural Sciences Nagoya University   ); Yokota, H.    (Laboratory of Animal Feeds and Production, University Farm, Graduate School of Bioagricultural Sciences Nagoya University  );
  • 초록

    Ghrelin is a novel growth-hormone-releasing acylated peptide, which has been purified and identified in rat stomach. In the present study, the full-length sequence of bovine ghrelin cDNA was cloned by RT-PCR. The bovine ghrelin cDNA sequence derived in the present study included a 348 bp open reading frame and a 137 bp 3'UTR. The putative amino acid sequence of bovine prepro-ghrelin consisted of 116 amino acids, which contained the 27-amino acid ghrelin. The sequence analysis of the bovine ghrelin gene revealed that an intron existed between Gln $^{13}$ and Arg $^{14}$ of ghrelin. This exon-intron boundary matched the GT-AG rule of the splicing mechanism. Compared with rats, which have two tandem CAG sequences in the 3'end of intron, bovine ghrelin genome has only one CAG sequence. Therefore, although rats can produce 28 amino acid-ghrelin and 27 amino acid-des-Gln $^{14}$ -ghrelin by alternative splicing, ruminant species, including bovines, might be able to produce only one type of ghrelin peptide, des-Gln $^{14}$ -ghrelin. The influence of aging on plasma ghrelin concentration was also examined. Plasma ghrelin concentration increased after birth to approximately 600 days of age, and then remained constant.


  • 주제어

    Ghrelin .   cDNA .   Genomic DNA .   Plasma Concentration .   Cattle.  

  • 참고문헌 (13)

    1. Kojima, M., H. Hosoda and K. Kangawa. 2001. Purification and distribution of ghrelin: The natural endogenous ligand for the growth hormone secretagogue receptor. Horm. Res. 56 (Suppl. 1):93-97. 
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    12. Hashizume, T., M. Horiuchi, N. Tate, S. Kojima, H. Hosoda and K. Kangawa. 2003. Effects of ghrelin on growth hormone secretion from cultured adenohypophysical cells in cattle. Endocrine J. 50:289-295. 
    13. Sakata, I., T. Tanaka, M. Matsubara, M. Yamazaki, S. Tani, Y. Hayashi, K. Kangawa and T. Sakai. 2002. Postnatal changes in ghrelin mRNA expression and in ghrelin-producing cells in the rat stomach. J. Endocrinol. 174:463-471. 

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