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The plant pathology journal v.21 no.2, 2005년, pp.127 - 131   피인용횟수: 2

The Effects of Temperature, pH, and Bactericides on the Growth of Erwinia pyrifoliae and Erwinia amylovora

Shrestha, Rosemary    (Division of Bio-Resources Technology, Kangwon National University   ); Lee, Seon-Hwa    (Division of Biological Environment, Kangwon National University   ); Hur, Jang-Hyun    (Division of Bio-Resources Technology, Kangwon National University   ); Lim, Chun-Keun    (Division of Bio-Resources Technology, Kangwon National University  );
  • 초록

    In this comparative study, the effects of temperature, pH, and bactericides on the growth of Erwinia pyrifoliae and Erwinia amylovora were investigated. The maximum temperature for the growth of both Erwinia species was estimated to be $36{\circ}C$ . The maximum specific growth rates of E. pyrifoliae and E. amylovora were observed at $27{\circ}C$ and $28{\circ}C$ , respectively, and no significant growth differences were shown at their optimum temperatures. However, at lower temperatures ranging from 12- $21{\circ}C$ , E. pyrifoliae showed higher growth rates with doubling times shorter than those of E. amylovora. Distinct growth rates at these temperatures revealed that E. pyrifoliae is more cold-tolerant than E. amylovora. The optimum pH for the growth of both pathogens was 7.5 and growth was not seen at pH ${\le}$ 5.0 and ${\ge}$ 10.0. These results showed that the effect of pH on the growth of E. pyrifoliae and E. amylovora was similar. Minimum inhibitory concentrations (MICs) of copper sulfate, oxolinic acid, streptomycin, and tetracycline, which inhibited growth of E. pyrifoliae and E. amylovora, were determined. The strains of both pathogens were able to grow at 0.08-0.32 mM copper sulfate, but not at higher concentrations. However, none of the tested strains grew in the presence of oxolinic acid (0.001 mM), streptomycin (0.1 mM), and tetracycline (0.01 mM) concentrations. These results suggested that all strains of both Erwinia species were sensitive to tested bactericides and indicated no occurrence of resistant strains of E. pyrifoliae in Korea.


  • 주제어

    doubling time .   $hrpN_Ep$ .   minimum inhibitory growth .   Pyrus pyrifolia .   shoot blight.  

  • 참고문헌 (19)

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    5. Ingraham, J. L., Maloe, O. and Neidhardt, F. C. 1983. Growth of bacterial cell. Sinauer Associates Inc. Massachusetts, USA 
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    16. Mc Manus, P. S., and Jones, A. L. 1994. Epidemiology and genetic analysis of streptomycin-resistant Erwinia amylovora from Michigan and evaluation of oxytetracycline for control. Phytopathology. 84:627-633 
    17. Shrestha, R., Tsuchiya, K., Baek, S. J., Bae, H. N., Hwang, I., Hur, J. H. and Lim, C. K. 2005. Identification of dspEF. hrpW, and hrpN loci and characterization of the $hrpN_{Ep}$ gene in Erwinia pyrifoliae. J. Gen. Plant Pathol. 71 (in press) 
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  • 이 논문을 인용한 문헌 (2)

    1. Lee, Sung Woo ; Lee, Seung Ho ; Park, Kyung Hoon ; Jang, In Bok ; Jin, Mei Lan ; Kim, Ki Hong 2014. "Effect of Irrigation of Sulfur Solution before Sowing on Growth and Root Rot Disease of Seedling in Ginseng Nursery" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 22(5): 391~397     
    2. Han, Kyu Suk ; Yu, Ji-Gang ; Lee, Han-Beoyl ; Oh, Chang-Sik ; Yea, Mi Chi ; Lee, Jong-Ho ; Park, Duck Hwan 2016. "Controlling by Effective Pruning of Twigs Showing Black Shoot Blight Disease Symptoms in Apple Trees" Research in plant disease = 식물병연구, 22(4): 269~275     

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