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The plant pathology journal v.21 no.2, 2005년, pp.142 - 148  

Biological Characterization and Sequence Analysis of Cucumber mosaic virus isolated from Capsicum annuum

Kim, Min-Jea    (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University   ); Choi, Seung-Kook    (USDA-ARS, Department of Plant Pathology, Cornell University   ); Yoon, Ju-Yeon    (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University   ); Choi, Jang-Kyung    (Division of Biological Environment, Kangwon National University   ); Ryu, Ki-Hyun    (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University  );
  • 초록

    Whereas most of isolates of Cucumber mosaic virus(CMV) can induce green mosaic systemic symptoms on zucchini squash, foliar symptoms of a pepper isolate of CMV (Pf-CMV)-infected zucchini squash revealed systemic chlorotic spots. To assess this biological property, infectious full-length cDNA clones of Pf-CMV were constructed using long-template RT-PCR. The complete nucleotide sequences of RNA2 and RNA3 of Pf-CMV were determined from the infectious fulllength cDNA clones, respectively. RNA 2 and RNA3 of Pf-CMV contain 3,070 nucleotides and 2,213 nucleotides, respectively. Overall sequence homology of two RNAs revealed high similarity (90%) between CMV strains, and 60% similarity to those of Tomato aspermy virus and Peanut stunt virus strains. By sequence analysis with known representative strains of CMV, Pf- CMV belongs to a typical member of CMV subgroup IA. The virus has high evolutionary relationship with Fny-CMV, but the pathology of Pf-CMV in zucchini squash was quite different from that of Fny-CMV. The pesudorecombinant virus, F1P2P3, induced chlorotic spot leaf symptom and timing of systemic symptom in squash plants, similar to the plants infected by Pf-CMV. No systemic symptoms were observed when Pf-CMVinoculated cotyledons were removed at 5 days postinoculation (dpi) while Fny-CMV showed systemic symptom at 2 dpi. These results suggest that the pepper isolate of CMV possesses unique pathological properties distinguishable to other isolates of CMVs in zucchini squash.


  • 주제어

    Cucumber mosaic virus .   Cucumovirus .   evolution .   pepper .   sequence analysis .   symptom .   virus movement.  

  • 참고문헌 (36)

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