본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

The plant pathology journal v.21 no.2, 2005년, pp.158 - 163   피인용횟수: 1

Virus-induced Gene Silencing as Tool for Functional Genomics in a Glycine max

Jeong, Rae-Dong    (Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University   ); Hwang, Sung-Hyun    (Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University   ); Kang, Sung-Hwan    (Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University   ); Choi, Hong-Soo    (Plant Pathology Div., National Institute of Agricultural Science and Technology, RDA   ); Park, Jin-Woo    (Plant Pathology Div., National Institute of Agricultural Science and Technology, RDA   ); Kim, Kook-Hyung    (Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University  );
  • 초록

    Virus-induced gene silencing (VIGS) is a recently developed gene transcript suppression technique for characterizing the function of plant genes. However, efficient VIGS has only been studied in a few plant species. In order to extend the application of VIGS, we examined whether a VIGS vector based on TRV would produce recognizable phenotypes in soybean. Here, we report that VIGS using the Tobacco rattle virus (TRV) viral vector can be used in several soybean cultivars employing various agro-inoculation methods including leaf infiltration, spray inoculation, and agrodrench. cDNA fragments of the soybean phytoene desaturase(PDS) was inserted into TRV RNA-2 vector. By agrodrench, we successfully silenced the expression of PDS encoding gene in soybean. The silenced phenotype of PDS was invariably obvious 3 weeks after inoculation with the TRV-based vector. Real-time RT-PCR analyses showed that the endogenous level of GmPDS transcripts was dramatically reduced in the silenced leaf tissues. These observations confirm that the silenced phenotype is closely correlated with the pattern of tissue expression. The TRV-based VIGS using agrodrench can be applied to functional genomics in a soybean plants to study genes involved in a wide range of biological processes. To our knowledge, this is the first high frequency VIGS method in soybean plants.


  • 주제어

    agro-inoculation .   agrodrench .   soybean .   Tobacco rattle virus .   VIGS.  

  • 참고문헌 (29)

    1. Andersen, S. U., Cvitanich, C., Hougaard, B. K., Roussis, A., Gronlund, M., Jensen, D. B., Frokjaer, L. A. and Jensen, E. O. 2003. The glucocorticoid-inducible GVG system causes severe growth defects in both root and shoot of the model legume Lotus japonicus. Mol. Plant-Microbe Interact. 16:1069-1076 
    2. Baulcombe, D. C. 1999. Fast forward genetics based on virusinduced gene silencing. Curr. Opin. Plant Biol. 2: 109-113 
    3. Ekengren, S. K., Liu, Y., Schiff, M., Dinesh-Kumar, S. P. and Martin, G B. 2003. Two MAPK cascades, NPR1, and TGA transcription factors playa role in Pto-mediated disease resistance in tomato. Plant J. 36:905-917 
    4. English, J. D. and Sweatt, J. D. 1997. A requirement for the mitogen-activated protein kinase cascade in hippocampal long term potentiation. J. Biol. Chem. 272: 19103-19706 
    5. Jin, H., Axtell, M. J., Dahlbeck, D., Ekwenna, O., Zhang, S., Staskawicz, B. and Baker, B. 2002. NPK1, an MEKK1-like mitogen-activated protein kinase kinase kinase, regulates innate immunity and development in plants. Dev. Cell 3:291-297 
    6. Liu, Y., Schiff, M., Marathe, R. and Dinesh-Kumar, S. P. 2002b. Tobacco Rarl, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to Tobacco mosaic virus. Plant J. 415-249 
    7. Matthews, R. E. F. 1991. Plant Virology, 3rd ed. San Diego: Academic Press 
    8. Ryu, C. M., Anand, A., Kang, L. and Mysore, K. S. 2004. Agrodrench: a novel and effective agroinoculation method for virus-induced gene silencing in roots and diverse Solanaceous species. Plant J. 40:322-331 
    9. Kumagai, H. and Kouchi, H. 2003. Gene silencing by expression of hairpin RNA in Lotus japonicus roots and root nodules. Mol. Plant Microbe Interact. 16:663-668 
    10. Dalmay, T., Hamilton, A, Mueller, E. and Baulcombe, D. C. 2000. Potato virus X amplicons in arabidopsis mediate genetic and epigenetic gene silencing. Plant Cell 12:369-379 
    11. Trieu, A. T., Burleigh, S. H. , Kardailsky, I. V, Maldonado-Mendoza, I. E., Versaw, W. K., Blaylock, L. A., Shin, H., Chiou, T. J., Katagi, H., Dewbre, G.R., Weigel, D. and Harrison, M. J. 2000. Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium. Plant J. 22:531-541 
    12. Brigneti, G., Martin-Hernandez, A.M., Jin, H., Chen, J., Baulcombe, D. C., Baker, B. and Jones, J. D. 2004. Virus-induced gene silencing in Solanum species. Plant J. 39: 264-272 
    13. MacFarlane, S. A. and Popovich, A. H. 2000. Efficient expression of foreign proteins in roots from tobravirus vectors. Virology 267:29-35 
    14. Dinesh-Kumar, S. P., Anandalakshmi, R., Marathe, R., Schiff, M. and Liu, Y. 2003. Virus-induced gene silencing. Methods Mol. Biol. 236:287-294 
    15. Demmig-Adams, B., Gilmore, A M. and Adams, W W, III. 1996. Carotenoids 3: in vivo function of carotenoids in higher plants. FASEB J. 10:41-45 
    16. Turnage, M. A., Muangsan, N., Peele, C. G. and Robertson, D. 2002. Geminivirus-based vectors for gene silencing in Arabidopsis. Plant J. 30: 107 -114 
    17. Chung, E., Seong, E., Kim, Y., C., Chung, E., Oh, S. K., Lee, S., Park, J.-M. and Choi, D. 2004. A method of high frequency virus-induced gene silencing in chill pepper (Capsicum annuum L. cv. Bukang). Mol. Cells 17:377-385 
    18. Jeong, R.-D., Lim, W-S., Kwon, S.-W. and Kim, K.-H. 2005. Identification of Glycine max genes expressed in response to Soybean mosaic virus infection. Plant Pathol. J. 21 :47-54     
    19. Lindbo, J. A., Fitzmaurice, W. P. and Della-Cioppa, G. 2001. Virus-mediated reprogramming of gene expression in plants. Curr. Opin. Plant Biol. 4: 181-185 
    20. Holzberg, S., Brosio, P., Gross, C. and Pogue, G. P. 2002. Barley stripe mosaic virus-induced gene silencing in a monocot plant. Plant J. 30:315-327 
    21. Lu, R., Martin-Hernandez, A. M., Peart, J. R., Malcuit, I. and Baulcombe, D. C. 2003. Virus-induced gene silencing in plants. Methods 30:296-303 
    22. Slaymaker, D. H., Navarre, D. A., Clark, D., del Pozo, O., Martin, G. B. and Klessig, D. F. 2002. The tobacco salicylic acid-binding protein 3 (SABP3) is the chloroplast carbonic anhydrase, which exhibits antioxidant activity and plays a role in the hypersensitive defense response. Proc. Natl. Acad. Sci. USA 99:11640-11645 
    23. Sharma, P. C., Ito, A., Shimizu, T., Terauchi, R., Kamoun, S. and Saitoh, H. 2003. Virus- I induced silencing of WIPK and SIPK genes reduces resistance to a bacterial pathogen, but has no effect on the INF1-induced hypersensitive response (HR) in Nicotiana benthamiana. Mol. Genet. Genomics 269:583-591 
    24. Gabriela, D., Britta N., Stuart, A., Mogens, N., Ida, E. J., Elisabeth J. and Ole, S. 2004. Virus-induced gene silencing as a tool for functional genomics in a legume species. Plant J. 40:622-631 
    25. Stiller, J., Martirani, L., Tuppale, S., Chian, R., Chiurazzi, M. and Gresshoff, P. 1997. High frequency transformation and regeneration of transgenic plants in the model legume Lotus japonicus. J. Exp. Bot. 48:1357-1365 
    26. Visser, P. B. and Bol, J. F. 1999. Nonstructural proteins of Tobacco rattle virus which have a role in nematode-transmission: expression pattern and interaction with viral coat protein. J. Gen. Virol. 80:3273-3280 
    27. Liu, Y., Schiff, M. and Dinesh-Kumar, S. P. 2002a. Virus-induced gene silencing in tomato. Plant J. 31 : 777-786 
    28. Ratcliff, F., Martin-Hernandez, A. M. and Baulcombe, D. C. 2001. Technical Advance. Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J. 25:237-245 
    29. Peart, J. R., Cook, G., Feys, B. J., Parker, J. E. and Baulcombe, D. C. 2002. An EDS1 orthologue is required for N-mediated resistance against tobacco mosaic virus. Plant J. 29:569-579 
  • 이 논문을 인용한 문헌 (1)

    1. 2007. "" The plant pathology journal, 23(3): 193~202     

 저자의 다른 논문

  • Jeong, Rae-Dong (1)

    1. 2005 "Identification of Glycine max Genes Expressed in Response to Soybean mosaic virus Infection" The plant pathology journal 21 (1): 47~54    
  • 황성현 (1)

  • 강성환 (0)

  • Kim, Kook-Hyung (24)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
  • 한국식물병리학회 : 저널
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기