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약학회지 = Yakhak hoeji v.49 no.2, 2005년, pp.122 - 127  

Streptomyces sp. M-20 균의 대사물에 의한 Streptococcus mutans의 Glucosyltransferase 활성 억제 효과
Inhibitory Effect of Metabolites isolated from Streptococcus mutas sp. M-20 on Glucosyltransferase Activity from Streptococcus mutans

김경자    (순천향대 자연과학대학 생명과학부  );
  • 초록

    Dental caries is one of the most common oral diseases in the world. Glucosyltransferase (Gtase) of Streptococcus mutans (S. mutans) plays an important role in the develo pment of dental caries. For the purpose to develop anti- caries, we examined the effect of metabolites isolated from Streptomyces sp. M-20 on Gtase and the growth of S. mutans. Streptomyces sp. M-20 isolated from Mongolian soil showed 95~96% sequence homology with that of Streptomyces lin- colnensis. The metabolites of Streptomyces sp. M-20 were partially purified by extraction with ethyl acetate, silica gel column chromatography and preparative TLC. Partially purified metabolite, red colored component (MR-20) in ethyl acetate fraction showed potent antibacterial activitiy against S. mutans and inhibitory activity against Gtase purified from S. mutans, while another isolated yellow component (MY-20) showed no activity against S. mutans. The inhibitory activity of MR-20 against Gtase was confirmed by activity staining on SDS-polyacrylamide gel electrophoresis. The concentration of MR-20 for 50% inhibition $(IC_{50})$ against Gtase activity was $60{\mu}g/ml$ . These results suggest that MR-20 can be developed for antibacterial agent and anticaries.


  • 주제어

    glucosyltransferase (GTase) .   Streptomyces sp.   M-20 .   Streptococcus mutans .   anticaries .   antimicrobial activity.  

  • 참고문헌 (30)

    1. Beighton, D. and Brailsford, S. R. : Plaque microbiology of root caries. In: Newman, H. N. and Wilson, M. (eds.). Dental plaque revisited Cardiff, Bioline. 283 (1999) 
    2. Hazlett, K. R., Mazurkiewicz, J. E. and Banas, J. A. : Inactivation of the gbpA gene of Streptococcus mutans alters structuraland functional aspects of plaque biofilm which are compensated by recombination of the gtfB and gtfC genes. Infect Immun. 67, 3930 (1999) 
    3. Ooshioma, T., Matsumura, M., Hoshino, T., Kawabata, S., Sobue, S. and Fujiwara, T. : Contributions of three glucosyltransferases to sucrose-dependent adherence of Streptococcus mutans. J. Dent. Res. 80, 1672 (2001) 
    4. Tagashira, M., Uchiyama, K, Yoshimura, T., Shirota, M. and Uemitsu, N. : Inhibition by hop bract polyphenols of cellular adherence and water-insoluble glucan synthesis of mutans Streptococci. Biosci. Biotechnol. Biochem. 61, 332 (1997) 
    5. Hamada, S., Kontani, M. and Hosono, H. : Peroxidasecatalysed generation of catechin oligomers that inhibit glucosyltranferase from Streptococcus sobrinus. FEMS Microbiol. 38, 717 (1990) 
    6. Koo, H., Hayacibara, M. E, Schobel, B. D., Cury, J. A., Rosalen, P. L., Park, Y. K., Vacca-Smith, A. M. and Bowen, W. H. : Inhibition of Streptoccus mutans biofilm accumulation and polysaccharide prodection by apigenin and tt-farnesol. J. Antimicrob. Chemother. 52, 782 (2003) 
    7. National Committee for Clinical Laboratory Standards: Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically 4th ed. Approved standard M7-A4. Villanova, Pennsylvania, National Committee for Clinical Laboratory Standards (1997) 
    8. Koo, H., Rosalen, P. L., Cury, J. A., Park, Y. K. and Bowen, W. H. : Effects of compounds found in propolis on Streptococci mutans growth and glucosyltransferase activity. Antimicrob. Agents. Chemother. 46, 1302 (2002) 
    9. Yanagida, A., Kanda, T, Tanabe, M., Matsudaira, F. and Oliveira, C. : Inhibitory effects of apple polyphenols and related compounds on cariogenic factors of mutants Streptococci. J. Agric. Food. Chem. 48, 5666 (2000) 
    10. Phan, T.N., Buckner, T., Sheng, J., Baldeck, T. D. and Marquis, R. E. : Physiologic actions of zinc related to inhibition of acid and alkali production by oral Streptococci in suspensions and biofilms. Oral Microbiol. Immun. 19, 31 (2004) 
    11. Nakahara, K., Kawabata, S. and Ono, H. : Inhibitory effect of oolong tea polyphenols on glucosyltransferases of mutans Streptococci. Appl. Environ. Microbiol. 59, 968 (1993) 
    12. Eto, A., Takaomi, C. S., Fukushima, K, Tomioka, S., Inai, S., Nisizawa, T and Hanada, N. : Inhibitory effect of a self-derived peptide on glucosyltransferase of Streptococcus mutans. J. Biol. Chem. 274, 15797 (1999) 
    13. Kubo, I., Muroi, H. and Himejima, M. : Antibacterial Activity aginst Streptococcus mutans of mate tea flavor components. J. Agric. Food Chem. 41, 107 (1993) 
    14. Hashimoto, K., Ynagi, K, Fukushima, K and Uda. Y. : Effect of 3-hydrocymethylene-2-thioxopyrrolidine on growth of two species of mutants streptococci and their in vitro plaque formation. Inter. J. Antimicrob. Agents. 17, 97 (2001) 
    15. Wunder, D. and Bowen, W. H. : Action of agents on glucosyltransferase from Streptococci mutants in solution and adsorbed to experimetal pellicle. Arch. Oral. Biol. 44, 203 (1999) 
    16. Radcliffe, C. E., Lamb, R., Blinkhorm, A. S. and Drucker, D. B. : Effect of sodium nitric and ascorbic acid production of Streptococcus mutans. J. Dent. 31, 367 (2003) 
    17. Kakiuchi, N., Hattori, M., Nishizawa, M., Yamagishi, T., Okuda, T. and Namba, T. : Studies on dental caries prevention by traditional medicines. VIII. Inhibitory effect of various tannins on glucan synthesis by glucosyltransferase from Streptococcus mutans. Chem. Pharm. Bull. 34, 720 (1986) 
    18. Kozai, K, Miyake, Y, Kohda, H., Kamataka, S., Yamasak, K, Suginakza, H. and Nagasaka, K. : Inhibition of glucosyltransferase from Streptococcus mutans by oleanolic acid and urosonic aid. Caries Res. 21, 104 (1987) 
    19. Koo, H., Rosalen, P. L., Cury, J. A., Park, Y. K. and Bowen, W. H. : Effects of compounds found in propolis on Streptococcus mutans growth and on glucosyltransferase activity. Antimicrob. Ag. Chemoth. 46, 1302 (2002) 
    20. Matsumoto, M., Minami, T., Sasaki, H., Sobue, S., Hamada, S. and Ooshima, T. : Inhibitory effects of oolong tea extract on caries inducing properties of mutans Streptococci. Caries Res. 3, 441 (1993) 
    21. Fujiwara, M., Hayashi, Y. and Ohara, N. : Inhibitory effect of water-soluble chitosan on growth of Streptococcus mutans. New Microbial. 27, 86 (2004) 
    22. Nguyen, P. T, Baldeck, J. D., Olsson, J. and Marquis, R. E. : Antimicrobial actions of benzimidazoles against oral Streptococci. Oral. Microbiol. Immunol. 20, 93 (2005) 
    23. Kamotsay, K, Herczegh, A., Rozgonyi, E, Nasz, I., Gintner, Z. and Banoczy. J. : Effect of fluoride on cariogenic oral microorganisms (an in vitro study). Acta Microbiol. Immunol Hung. 49, 47 (2002) 
    24. Laemmli, U. K. : Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680 (1970) 
    25. Park, C. S., Shin, Y. S., Ryu, I. W. and Lee, K. S. : Antimicrobial activity of extract from aloe vera peel against Streptococcus mutans JC-2. Korean J. Food & Nutr. 13, 139 (2000) 
    26. Minami, T., Fujiwara, T., Ooshima, T. and Hamada, S. : Interaction of structural isomers of sucrose in the reaction between sucrose and glucosyltransferases from mutans Streptococci. Oral Microbiol. Immunol. 5, 189 (1990) 
    27. Hardie, J. M. and Whiley, R. A. : Plaque microbiology of crown caries. In: Newman, H. N. and Wilson, M. (eds.). Dental plaque revisited Cardiff, Bioline. 283 (1999) 
    28. Ohk, S. H., Yoo. Y. J. and Bai, D. H. Purification and characterization of Streptococcus mutans cell wall hydrolase from Bacillus subtilis YL-1004. J. Microbiol. Biotechnol. 11, 957 (2001) 
    29. Kim, J. H., Lee, D. S., Lim, C. W, Park, H. Y. and Park, J. H. : Antibacterial activity of sea-mustard, laminaria japonica extracts on the cariogenic bacteria, Strptococcus mutans. J. Korean Fish. Soc. 35, 191 (2002) 
    30. Kubo, I., Muroi, H. and Kubo, A. : Antibacterial activity of longchain alcohols against Streptococcus mutans. J. Agric. Food Chem. 41, 2447 (1993) 

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