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In Vitro Free Radical and ONOO- Scavengers from Sophora flavescens

Jung, Hee-Jin    (Faculty of Food Science and Biotechnology, Pukyong National University   ); Kang, Sam-Sik    (Natural Products Research Institute, Seoul National University   ); Hyun, Sook-Kyung    (Faculty of Food Science and Biotechnology, Pukyong National University   ); Choi, Jae-Sue    (Faculty of Food Science and Biotechnology, Pukyong National University  );
  • 초록

    Activity-guided fractionation of the CH $_2Cl_2$ -soluble fraction of the roots of Sophora flavescens furnished five 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scaveng ers: trans-hexadecyl ferulic acid (1) cis-octadecyl ferulic acid (2), trans-hexadecyl sinapic acid (3), (-)-4-hydroxy-3-methoxy-(6aR,11aR)-8, 9-methylenedioxypterocarpan (4) and desmethylanhydroicaritin (8), along with nine known inactive compounds: (-)-maackiain (5), xanthohumol (6), formononetin (7), (2S)-2'-methoxykurarinone (9), (2S)-3 ${\beta}$ ,7,4'-trihydroxy-5-methoxy-8-( ${\gamma},{\gamma}$ - imethylallyl )-flavanone (10), (2S)-7,4'-dihydroxy-5-methoxy-8- ( ${\gamma},{\gamma}$ -dimethylallyl ) -flavanone (11), umbelliferone (12), kuraridin (13), and trifolirhizin (14). Compounds 1-4 and 8 exhibited DPPH free radical scavenging effects at IC $_{50}$ values of 33.01 ${\pm}$ 0.20, 57.06 ${\pm}$ 0.16, 39.84 ${\pm}$ 0.36, 35.83 ${\pm}$ 0.47, and 18.11 ${\pm}$ 0.04 ${\mu}$ M, respectively. L-Ascorbic acid, when used as a positive control, exhibited an IC $_{50}$ value of 7.39 ${\pm}$ 0.01 ${\mu}$ M. Compounds 1-4 and 8 also appeared to exert significant scavenging effects on authentic ONOO-, with IC $_{50}$ values of 5.76 ${\pm}$ 1.19, 15.06 ${\pm}$ 1.64, 8.17 ${\pm}$ 4.97, 1.95 ${\pm}$ 0.29 and 4.06 ${\pm}$ 2.41 ${\mu}$ M, respectively. Penicillamine (IC $_{50}$ = 2.36 2.36 ${\pm}$ 0.79 ${\mu}$ M) was used as a positive control. In addition, compounds 2,4,6,8, and 10 were isolated from this plant for the first time.


  • 주제어

    Sophora flavescens .   1,1-Diphenyl-2-picrylhydrazyl radical scavengers .   ONOO scavengers .   cis-Octadecyl ferulic acid .   (-)-4-Hydroxy-3-methoxy-(6aR, 11aR)-8,9-methylene-dioxypterocarpan.  

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