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Triterpenoids from the Flower of Campsis grandiflora K. Schum. As Human Acyl-CoA: Cholesterol Acyltransferase Inhibitors

Kim, Dong-Hyun    (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University   ); Han, Kyung-Min    (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University   ); Chung, In-Sik    (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University   ); Kim, Dae-Keun    (Department of Pharmacy, Woosuk University   ); Kim, Sung-Hoon    (Graduate School of East-West Medical Science, Kyung Hee University   ); Kwon, Byoung-Mog    (Korea Research Institute of Bioscience and Biotechnology   ); Jeong, Tae-Sook    (Korea Research Institute of Bioscience and Biotechnology   ); Park, Mi-Hyun    (Erom life Co. Ltd.   ); Ahn, Eun-Mi    (Scigenic Co. Led.   ); Baek, Nam-In    (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University  );
  • 초록

    The flower of Campsis grandiflora K. Schum. Was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H $_2$ O. From the EtO Ac fraction, seven triterpenoids were isolated through the repeated silica gel, ODS column chromatographies and preparative HPLC. From the result of physico- chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as 3 ${\beta}$ -hydroxyolean-12-en-28-oic acid (oleanolic acid, 1), 3 ${\beta}$ -hydroxyurs-12-en-28-oic acid (ursolic acid, 2), 3 ${\beta}$ -hydroxyurs-12-en-28-al (ursolic aldehyde, 3), 2 ${\alpha}$ ,3 ${\beta}$ -dihydroxyolean-12-en-28-oic acid (maslinic acid, 4), 2 ${\alpha}$ ,3 ${\beta}$ -dihydroxyurs-12-en-28-oic acid (corosolic acid, 5), 3 ${\beta}$ ,23-dihydroxyurs-12- en-28-oic acid (23-hydroxyursolic acid ,6) and 2 ${\alpha}$ ,3 ${\beta}$ ,23-trihydroxyolean-12-en-28- oic acid (arjunolic acid, 7). These teriterpenoids were isolated for the first time from this plant. Also, compounds 4, 5, 6, and 7 revealed relatively high hACAT-1 inhibitory activity with the value of 46.2 ${\pm}$ 1.1, 46.7 ${\pm}$ 0.9, 41.5 ${\pm}$ 1.3 and 60.8 ${\pm}$ 1.1% at the concentration of 100 ${\mu}$ g/mL, respectively.


  • 주제어

    Campsis grandiflora .   Bignoniaceae .   hACAT1 Inhibitory effect .   Oleanolic acid .   Ursolic acid.  

  • 참고문헌 (27)

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  • 이 논문을 인용한 문헌 (5)

    1. 2007. "" Bulletin of the Korean Chemical Society, 28(10): 1851~1853     
    2. Lee, Dae-Young ; Jeong, Tae-Sook ; Lyu, Ha-Na ; Bang, Myun-Ho ; Hong, Yoon-Hee ; Lee, Youn-Hyung ; Baek, Nam-In 2008. "Screening for Human ACAT-1 and ACAT-2 Inhibitory Activity of Edible Plant Extracts" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 16(5): 341~348     
    3. 2009. "" Natural product sciences, 15(2): 90~95     
    4. 2009. "" Natural product sciences, 15(3): 151~155     
    5. 2010. "" Journal of the Korean Society for Applied Biological Chemistry, 53(1): 97~100     

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  • 김성훈 (154)

  • Kwon, Byoung-Mog (27)

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