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Improvement of Menopausal Signs by Isoflavones Derived from Sophorae fructus in Ovariectomized Female Rats and the Antioxidant Potentials in BV2 Cells

Joo, Seong-Soo    (College of Pharmacy, Chung-Ang University   ); Kwon, Suk-Hyung    (Institute for Life Science, Rexgene Biotech   ); Hwang, Kwang-Woo    (College of Pharmacy, Chung-Ang University   ); Lee, Do-Ik    (College of Pharmacy, Chung-Ang University  );
  • 초록

    The aim of this study was to determine if the isoflavones from Sophorae fructus (SISO) have potential clinical benefit in hormone replacement therapy (HRT) for the treat ment of menopausal signs, such as the levels of total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL) and follicle stimulating hormone (FSH). An additional aim was to present the potential antioxidant effect of SISO in a microglial cell line. For the animal model, the ovaries were removed from adult rats and the indicators of menopause were measured at the pre- and post-administration time points. Although no statistically significant correlation was found, SISO tended to decrease the TC level (p=0.15) and the FSH level (p=0.36), but to increase the HDL level (p=0.303).SISO ( ${\mu}g$ /mL) also exerted antioxidant activity on BV-2 microglial cells by inhibiting lipopolysaccharide-induced nitric oxide. This cytoprotective effect was confirmed by trypan blue staining, which was used to test for cellular damage from H $_2O_2$ . In conclusion, this study highlights the anti-menopausal and antioxidant effect of SISO in an ovariectomized rat model, as well as in microglial cells, and provides new clinical targets for the screening of phytoestrogens as potential candidates for HRT in menopausal women.


  • 주제어

    Sophorae fructus .   Phytoestrogen .   Hormone replacement therapy .   Menopause .   Climacterics.  

  • 참고문헌 (17)

    1. Beral, V., Banks, E., Reeves, G., and Appleby, P., Use of HRT and the subsequent risk of cancer. J. Epidemiol. Biostat., 4, 191-215 (1999) 
    2. Brownson, D. M., Azios, N. G., Fuqua, B. K., Dharmawardhane, S. F., and Mabry, T. J., Flavonoid effects relevant to cancer. J. Nutr., 132, 3482S-3489S (2002) 
    3. Joo, S. S., Won, T. J., Kang, H. C., and Lee, D. I., Isoflavones extracted from Sophorae fructus upregulate IGF-1 and TGF- $\beta$ and inhibit osteoclastogenesis in rat bone marrow cells. Arch. Pharm. Res., 27, 99-105 (2004)     
    4. Kuiper, G., Lemmen, J., Carlsson, B., Corton, J. C., Safe, S. H., van der Saag, P. T., van der Burg, B., and Gustafsson, J. A., Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta. Endocrinology, 139, 4252-4264 (1998) 
    5. Teresa, C., Cohick, W., and Raskin, I., Dietary phytoestrogens and health. Phytochemistry, 65, 995-1016 (2004) 
    6. Wuttke, W., Jarry, H., Becker, T., Schultens, A., Christoffel, V., Gorkow, C., and Seidlova-Wuttke, D., Phytoestrogens: endocrine disruptors or replacement for hormone replacement therapy? Maturitas, 44, S9-S20 (2003) 
    7. Fitzpatric, L. A., Soy isoflavones: hope or hype? Maturitas, 44, 21-29 (2003) 
    8. Kronenberg, F. and Fugh-Berman, A., Complementary and alternative medicine for menopausal systems: a review of randomized, controlled trials. Ann. Intern. Med., 137, 805- 813 (2002) 
    9. Adlercreutz, H., Epidemiology of phytoestrogens. Baillieres Clin. Endocrinol. Metab., 12, 605-623 (1998) 
    10. Cassidy, A., Bingham, S., and Setchell, K. D., Biologic effects of a diet of soy protein rich in isoflavones on the menstrual cycle of premenopausal women. Am. J. Clin. Nutr., 60, 333-340 (1994) 
    11. Kang, H. J., Ansbacher, R., and Hammoud, M. M., Use of alternative and complementary medicine in menopause. Intern. J. Gynecol. Obstet., 79, 195-207 (2002) 
    12. Steer, T. E., Johnson, I. T., Gee, J. M., and Gibson, G.. R., Metabolism of the soybean isoflavone glycoside genistin in vitro by human gut bacteria and the effect of prebiotics. Br. J. Nutr., 90, 635-642 (2003) 
    13. Chlebowski, R. T., Hendrix, S. L., Langer, R. D., Stefanick, M. L., Gass, M., Lane, D., Rodabough, R. J., Gilligan, M. A., Cyr, M. G., Thomson, C. A., Khandekar, J., Petrovitch, H., and McTiernan, A., Influence of estrogen plus progesterone on breast cancer and mammography in healthy postmenopausal women. JAMA, 289, 3243-3253 (2003) 
    14. Greendale, G. A., Reboussin, B. A., Hogan, P., Barnabei, V. M., Shumaker, S., and Barrett-Connor, E., Symptom relief and side effects of postmenopausal hormone results from the postmenopausal estrogen/progestin interventions trial. Obstet. Gynecol., 92, 982-988 (1998) 
    15. Greendale, G. A., Lee, N. P., and Arriola, E. R., The menopause. Lancet, 353, 571-580 (1999) 
    16. Joo, S. S., Won, T. J., and Lee, D. I., Potential role of ursodeoxycholic acid in suppression of nuclear factor kappa B in microglial cell line (BV-2). Arch. Pharm. Res., 27, 954-960 (2004)     
    17. Tavani, A. and La Vecchia, C., The adverse effects of hormone replacement therapy. Drugs Aging, 14, 347-357 (1999) 
  • 이 논문을 인용한 문헌 (2)

    1. Kang, Young-Soon ; Han, Min Ho ; Lee, Moon Hee ; Hong, Su Hyun ; Park, Heungsik ; Jung, Jae-Chul ; Lee, Jeongrai ; Lee, Eun-Woo ; Kang, Kyung Hwa ; Kim, Cheol Min ; Kim, Byung-Woo ; Choi, Yung Hyun 2013. "Fructus Sophorae Enhances the Production of Prostaglandin E2 and Tumor Necrosis Factor-α through Activation of MAPKs and PI3K/AKT Signaling Pathways in Murine Macrophages" 생명과학회지 = Journal of life science, 23(11): 1397~1403     
    2. 2016. "" Applied Biological Chemistry = 한국응용생명화학회지, 59(4): 525~532     

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