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Identification And Quantification of Steroidal Saponins in Polygonatum Species by HPLC/ESI/MS

Ahn, Mi-Jeong    (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University   ); Kim, Jin-Woong    (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University  );
  • 초록

    An HPLC/Esl/MS method has been developed to identify and quantify the spirostanol glycosides in the rhizomes of five Polygonatum species. The relative distribution of two steroidal saponins in each extract was established using the selective ion monitoring (SIM) mode via an electrospray ionization (ESI) source. It was found that there were significant differences in the amount of spirostanol glycosides among the Polygonatum Species. The results showed that this method could be used to identify the steroidal saponins in the extracts and differentiate Polygonatum species with high sensitivity and reproducibility in a short time. Fragmentation patterns of the two reference compounds were also discussed with the electrospray ionization multi-stage tandem mass spectroscopy (ESI-MS $^n$ ).


  • 주제어

    HPLC/ESI/MS .   Polygonatum species .   Spirostanol glycosides .   SIM mode .   ESI-MS $^n$.  

  • 참고문헌 (14)

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    5. Liu, Y. P., Komatsu, K., Xie, Z. W., and Namba, T., Pharmacognostical studies on the Polygonatum plants. (part IV) On the botanical origins of Chinese crude drugs 'Yuzhu', 'Huangjing', and related drugs from China. Shoyakugaku Zasshi, 47, 249-266 (1993b) 
    6. Miura, T. and Kato, A., The difference in hypoglycemic action between Polygonati Rhizoma and Polygonati Officinalis Rhizoma. Biol. Pharm. Bull., 18, 1605-1606 (1995) 
    7. Waller, G. R. and Yamasaki, K. (Eds.), Saponins used in food and agriculture, In Advances in Experimental Medicine and Biology, Vol. 405, Plenum Press, New York (1996a) 
    8. Jang, C.-G. and Kim, Y.-S., Taxanomic relationships of the Korean Polygonatum (Liliaceae) using the RAPDs analysis. Kor. J. Plant Tax., 28, 371-384 (1998) 
    9. Anonymous, Dictionary of Chinese Medicinal Materials, Shanghai Scientific and Technological Press, Shanghai, Vol. 2, pp. 2041-2044 (1978) 
    10. Liu, Y. P., Komatsu, K., Park, J. H., Mikage, M., and Namba, T., Pharmacognostical studies on the Polygonatum plants. (part II) On the botanical origins of the crude drug 'Hwangjeong' from Korea. Shoyakugaku Zasshi, 47, 131-143 (1993a) 
    11. Waller, G. R. and Yamasaki, K. (Eds.), Saponins used in traditional and modern medicine, In Advances in Experimental Medicine and Biology, Vol. 404, Plenum Press, New York (1996b) 
    12. Jang, K. H., Kang, J. H., Jeon, B. S., Song, G. W., and Lee, S. D., Major chemical components in rhizome of native Polygonatum species in Korea. Korean J. Medicinal Crop Sci., 10, 185-193 (2002)     
    13. Ono, M., Shoyama, K., and Nohara, T., The constituents of Chinese Polygonati Officinalis Rhizoma and Polygonati Rhizoma. Shoyakugaku Zasshi, 42, 135-142 (1988) 
    14. Kato, A. and Miura, T., Hypoglycemic activity of Polygonati rhizoma in normal and diabetic mice. Biol. Pharm. Bull., 16, 1118-1120 (1993) 

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  • Kim, Jin-Woong (45)

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    2. 1981 "이리도이드 배당체(配糖體) (II) -속단(續斷)의 이리도이드 배당체(配糖體) 및 진형과(唇形科) 식물(植物)의 제암효과(制癌效果)-" 생약학회지 12 (2): 82~87    
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    9. 1992 "생약으로부터 항암성분의 검색 및 분리 (I)" 생약학회지 23 (4): 264~267    
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