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Journal of microbiology and biotechnology v.15 no.1, 2005년, pp.99 - 104   피인용횟수: 4

Action Mechanism of Transfructosylation Catalyzed by Microbacterium laevaniformans Levansucrase

KIM, MIN-JEONG    (Department of Microbiology, Pusan National University   ); PARK, HAE-EUN    (Department of Microbiology, Pusan National University   ); SUNG, HEE-KYUNG    (Department of Microbiology, Pusan National University   ); PARK, TACK-HYUN    (Department of Microbiology, Pusan National University   ); CHA, JAE-HO    (Department of Microbiology, Pusan National University  );
  • 초록

    Microbacterium laevaniformans levansucrase synthesized various hetero-oligosaccharides by transferring fructosyl residue from sucrose to various saccharides as acceptors. The acceptor specificity test showed that reducing saccharides were more favorable acceptors than nonreducing saccharides. The transfructosylated product, fructosyl galactose, was produced in the presence of D-galactose as an acceptor. The chemical structure of the resulting fructosyl galactose was analyzed by yeast invertase and NMR, and identified as O- $\alpha$ -D-galactosyl-(1 ${\to}$ 2)- $\beta$ -D-fructofuranoside. These results indicate that the main transfructosylation activity of the enzyme is to make nonreducing transferred products via a transfer of fructosyl residue to acceptor molecules having reducing group. When nonreducing sugars, such as methyl $\alpha$ -D-glucoside and methyl $\alpha$ -D-galactoside, were used as an acceptor, the transfer product was also formed in spite of the reducing group blocked with methyl group. The fact that no transfer product was formed with sugar alcohols as acceptors was suggested to be due to marked conformational difference of acceptors.


  • 주제어

    Levansucrase .   transfructosylation .   heterooligosaccharide.  

  • 참고문헌 (18)

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  • 이 논문을 인용한 문헌 (4)

    1. 2007. "" Journal of microbiology and biotechnology, 17(11): 1751~1757     
    2. 2007. "" Journal of microbiology and biotechnology, 17(5): 792~799     
    3. 2008. "" Journal of microbiology and biotechnology, 18(6): 1064~1069     
    4. 2009. "" Journal of microbiology and biotechnology, 19(10): 1153~1160     

 저자의 다른 논문

  • KIM, MIN-JEONG (2)

    1. 2003 "Enzymatic Characterization of a Recombinant Levansucrase from Rahnella aquatilis ATCC 15552" Journal of microbiology and biotechnology 13 (2): 230~235    
    2. 2003 "Isolation and Characterization of Mucinase Complex Secreted from Vibrio parahaemolyticus" Journal of microbiology and biotechnology 13 (5): 731~737    
  • PARK, HAE-EUN (1)

  • SUNG, HEE-KYUNG (1)

  • Cha, Jae-Ho (27)

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