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Journal of microbiology and biotechnology v.15 no.1, 2005년, pp.48 - 54   피인용횟수: 6

Comparison of Photorhabdus luminescens and Vibrio fischeri lux Fusions to Study Gene Expression Patterns

MITCHELL, ROBERT J.    (National Research Laboratory on Environmental Biotechnology, Depart. Environmental Science and Engineering, Institute of Science and Technology (GIST)   ); AHN, JOO-MYUNG    (National Research Laboratory on Environmental Biotechnology, Depart. Environmental Science and Engineering, Institute of Science and Technology (GIST)   ); GU, MAN BOCK    (National Research Laboratory on Environmental Biotechnology, Depart. Environmental Science and Engineering, Institute of Science and Technology (GIST)  );
  • 초록

    A comparison of promoter fusions with the luxCDABE genes from Vibrio fischeri and Photorhabdus luminescens was made using promoters from several genes (katG, sodA, and pqi-5) of E. coli that are responsive to oxidative damage. The respective characteristics, such as the basal and maximum bioluminescence and the relative bioluminescence, were compared. E. coli strains carrying fusions of the promoters to P. luminescens lux showed higher basal and maximally induced bioluminescent levels than strains carrying the same promoter fused to the luxCDABE genes from V. fischeri. The sensitivities between the strains were similar, regardless of the luciferase used, but lower response ratios were seen from strains harboring the P. luminescens lux fusions. Furthermore, using the two katG::lux fusion strains, the bioluminescence from the P. luminescens lux fusion strain, DK1, was stable after reaching a maximum, while that of strain DPD2511 decreased very rapidly due to substrate limitation.


  • 주제어

    P.   luminescens .   V.   fischeri .   luxCDABE .   gene fusions .   bioluminescence .   oxidative damage.  

  • 참고문헌 (34)

    1. Almashanu, S., A. Tuby, R. Hadar, R. Einy, and J. Kuhn. 1995. Formation of active bacterial luciferase between interspecific subunits in vivo. J. Biolumin. Chemilumin. 10: 157-167 
    2. Meighen, E. A. 1991. Molecular biology of bacterial bioluminescence. Microbial. Rev. 55: 123- 142 
    3. Park, J. E., K.-H. Lee, and D. Jahng. 2002. Effect of trehalose on bioluminescence and viability of freeze-dried bacterial cells. J. Microbiol. Biotechnol. 12: 349- 353 
    4. Pazzagli, M., J. H. Devine, D. O. Peterson, and T. O. Baldwin. 1992. Use of bacterial and firefly luciferases as reporter genes in DEAE-dextran-mediated transfection of mammalian cells. Anal. Biochem. 204: 315- 323 
    5. Hakkila, K, M. Maksimow, M. Karp, and M. Virta. 2002. Reporter genes lucFF, luxCDABE, gfp, and dsred have different characteristics in whole-cell bacterial sensors. Anal. Biochem. 301: 235- 242 
    6. Davidov, Y., R. Rozen, D. R. Smulski, T. K. Van Dyk, A. C. Vollmer, D. A. Elsernore, R. A. LaRossa, and S. Belkin. 2000. Improved bacterial SOS promoter::lux fusions for genotoxicity detection. Mutat. Res. 466: 97-107 
    7. Szittner, R. and E. Meighen. 1990. Nucleotide sequence, expression, and properties of luciferase coded by lux genes from a terrestrial bacterium. J. Biol. Chem. 265: 16581-16587 
    8. Winson, M. K, S. Swift, P. J. Hill, C. M. Sims, G. Griesrnayr, B. W. Bycroft, P Williams, and G. S. Stewart. 1998. Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs. FEMS Microbiol Lett. 163: 193- 202 
    9. Xi, L. and S. C. Tu. 1993. Construction and characterization of hybrid luciferases coded by lux genes from Photorhabdus luminescens and Vibrio fischeri. Photochem. Photobiol. 57: 714-719 
    10. Mitchell, R. J. and M. B. Gu. 2004. An Escherichia coli biosensor capable of detecting both genotoxic and oxidative damage. Appl. Microbiol. Biotechnol. 64: 46- 52 
    11. Lee, H. J. and M. B. Gu. 2003. Construction of a sodA::luxCDABE fusion Escherichia coli: Comparison with a katG fusion strain through their responses to oxidative stresses. Appl. Microbiol. Biotechnol. 60: 577- 580 
    12. Van Dyk, T. K., Y. Wei, M. K. Hanafey, M. Dolan, M. J. Reeve, J. A. Rafalski, L. B. Rothman-Denes, and R. A. LaRossa. 2001. A genomic approach to gene fusion technology. Proc. Natl Acad. Sci. USA 98: 2555- 2560 
    13. Demple, B. 1991. Regulation of bacterial oxidative stress genes. Annu. Rev. Genet. 25: 315- 337 
    14. Storz, G., S. B. Farr, and B. N. Ames. 1990. Bacterial defenses against oxidative stress. Trends Genet. 6: 363- 368 
    15. Delong, E. F., D. Steinhauer, A. Israel, and K. H. Nealson. 1987. Isolation of the lux genes from Photobacterium leiognathi and expression in Escherichia coli. Gene 54: 203- 210 
    16. Manukhov, I. V., G. E. Eroshnikov, M. Y. Vyssokikh, and G. B. Zavilgelsky. 1999. Folding and refolding of thermolabile and thermostable bacterial luciferases: The role of DnaKJ heat-shock proteins. FEBS Lett. 448: 265- 268 
    17. Van Dyk, T. K and R. A. Rosson. 1998. 102: 85- 95. In R. A LaRossa (ed.), Methods in Molecular Biology: Bioluminescence Methods and Protocols. Humana Publishing, Towowa, NJ, U.S.A. 
    18. Rogowsky, P. M., T. J. Close, J. A. Chimera, J. J. Shaw, and C. I. Kado. 1987. Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58. J. Bacteriol. 169: 5101-5112 
    19. Rupani, S. P, M. B. Gu, K. B. Konstantinov, P. S. Dhurjati, T. K. Van Dyk, and R. A. LaRossa. 1996. Characterization of the stress response of a bioluminescent biological sensor in batch and continuous cultures. Biotechnol Prog. 12: 387-392 
    20. Frackrnan, S., M. Anhalt, and K. H. Nealson. 1990. Cloning, organization, and expression of the bioluminescence genes of Photorhabdus luminescens. J. Bacteriol. 172: 5767- 5773 
    21. Korbashi, P., R. Kohen, J. Katzhendler, and M. Chevion. 1986. Iron mediates paraquat toxicity in Escherichia coli. J. Biol. Chem. 261: 12472- 12476 
    22. Stewart, G. S. A. B. and P. Williams. 1992. lux Genes and the applications of bacterial bioluminescence. J. Genet. Microbiol. 138: 1289- 1300 
    23. Tyulkova, N. A. and T. P. Sandalova. 1996. Comparative study of temperature effects on bacterialluciferase. Biochemistry (Moscow) 61: 205- 214 
    24. Min, J. and M. B. Gu. 2003. Genotoxicity assay using chromosomally-integrated bacterial recA::Lux. J. Microbiol. Biotechnol. 13: 99- 103     
    25. Greenberg, J. T., P. Monach, J. H. Chou, P. D. Josephy, and B. Demple. 1990. Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli. Proc. Natl. Acad. Sci. USA 87: 6181-6185 
    26. Belkin, S., D. R. Srnulski, A. C. Vollmer, T. K. Van Dyk, and R. A. LaRossa. 1996. Oxidative stress detection with Escherichia coli harboring a katG':lux fusion. Appl. Environ. Microbiol. 62: 2252-2256 
    27. Premkurnar, J. R., R. Rosen, S. Belkin, and O. Lev. 2002. Sol-gel luminescence biosensors: Encapsulation of recombinant E. coli reporters in thick silicate gels. Anal. Chim. Acta 462: 11- 23 
    28. Cho, J. C. and S. J. Kim. 1999. Green tluorescent proteinbased direct viable count to verify a viable but nonculturable state of Salmonella typhi in environmental samples. J. Microbiol. Methods 36: 227- 235 
    29. Meighen, E. A. and P. V. Dunlap. 1993. Physiological, biochemical and genetic control of bacterial bioluminescence. Adv. Microbial Physiol. 34: 2- 58 
    30. Baldwin, T. O., J. H. Devine, R. C. Heckel, J. W. Lin, and G. S. Shadel. 1989. The complete nucleotide sequence ofthe lux regulon of Vibrio fischeri and the luxABN region of Photobacterium leiognathi and the mechanism of control of bacterial bioluminescence. J. Biolumin. Chemilumin. 4: 326- 341 
    31. Lee, H. Y, S. H. Choi, and M. B. Gu. 2003. Response of bioluminescent bacteria to sixteen azo dyes. Biotechnol. Bioprocess Eng. 8: 101- 105 
    32. Van Dyk, T. K., E. J. DeRose, and G. E. Gonye. 2001. LuxArray, a high-density, genomewide transcription analysis of Escherichia coli using bioluminescent reporter strains. J. Bacteriol. 183: 5496- 5505 
    33. Engebrecht, J. and M. Silverman. 1984. Identification of genes and gene products necessary for bacterial bioluminescence. Proc. Natl. Acad. Sci. USA 81: 4154- 4158 
    34. Justus, T. and S. M. Thomas. 1999. Evaluation of transcriptional fusions with green fluorescent protein versus luciferase as reporters in bacterial mutagenicity tests. Mutagenesis 14: 351- 356 
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    6. 2010. "" BMB reports, 43(7): 451~460     

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