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Journal of microbiology and biotechnology v.15 no.1, 2005년, pp.72 - 79   피인용횟수: 16

Purification and Characterization of a New Peptidase, Bacillopeptidase DJ-2, Having Fibrinolytic Activity: Produced by Bacillus sp. DJ-2 from Doen-Jang

CHOI, NACK-SHICK    (Proteome and Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Department of Microbiology, Chungnam National University   ); YOO, KI-HYUN    (Proteome and Research Laboratory, Korea Research Institute of Bioscience and Biotechnology   ); HAHM, JEUNG-HO    (Proteome and Research Laboratory, Korea Research Institute of Bioscience and Biotechnology   ); YOON, KAB-SEOG    (Proteome and Research Laboratory, Korea Research Institute of Bioscience and Biotechnology   ); CHANG, KYU-TAE    (Primate Research Laboratory, Korea Research Institute of Bioscience and Biotechnology   ); HYUN, BYUNG-HWA    (Primate Research Laboratory, Korea Research Institute of Bioscience and Biotechnology   ); PIL, JAE-MAENG    (Department of Microbiology, Chungnam National University   ); KIM, SEUNG-HO    (Proteome and Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ;  );
  • 초록

    A new Bacillus peptidase, bacillopeptidase DJ-2 (bpDJ-2), with molecular mass of 42 kDa and isoelectric point (pI) of 3.5- 3.7, was purified to homogeneity from Bacillus sp. DJ-2 isolated from Doen-Jang, a traditional Korean soybean fermented food. The enzyme was identified as an extracellular serine fibrinolytic protease. The optimal conditions for the reaction were pH 9.0 and $60^{\circ}C$ . The first 18 amino acid residues of the N-terminal amino acid sequence of bpDJ-2 were TDGVEWNVDQIDAPKAW, which is identical to that of bacillopeptidase F (bpf). However, based on their Nterminal amino acid sequence, molecular size, and pI, it is different from that of bpf and extracellular 90 kDa. The whole (2,541 bp, full-bpDJ-2) and mature (1,956 bp, mature-bpDJ-2) genes were cloned, and its nucleotide sequence and deduced amino acid sequence were determined. The expressed proteins, full-bpDJ-2 and mature-bpDJ-2, were detected on SDSPAGE at expected sizes of 92 and 68 kDa, respectively.


  • 주제어

    Bacillopeptidase .   Bacillus sp.   DJ-2 .   Doen-Jang .   fibrinolytic enzyme .   serine protease .   zymography.  

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