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Journal of microbiology and biotechnology v.15 no.1, 2005년, pp.131 - 135   피인용횟수: 4

Cell Age Optimization for Hydrogen Production Induced by Sulfur Deprivation Using a Green Alga Chlamydomonas reinhardtii UTEX 90

KIM , JUN-PYO    (Department of Chemical Engineering, Sungkyunkwan University   ); KANG, CHANG-DUK    (School of Chemical Engineering, Seoul National University   ); SIM, SANG-JUN    (Department of Chemical Engineering, Sungkyunkwan University   ); KIM, MI-SUN    (Biomass Research Team, Korea Institute of Energy Research   ); PARK, TAI-HYUN    (School of Chemical Engineering, Seoul National University   ); LEE, DONG-HYUN    (Department of Chemical Engineering, Sungkyunkwan University   ); KIM, DUK-JOON    (Department of Chemical Engineering, Sungkyunkwan University   ); KIM, JI-HEUNG    (Department of Chemical Engineering, Sungkyunkwan University   ); LEE, YOUNG-KWAN    (Department of Chemical Engineering, Sungkyunkwan University   ); PAK, DAE-WON    (Water Environment and Remediation Research Center, Korea Institute of Science and Technology  );
  • 초록

    Under sulfur deprived conditions, PS II and photosynthetic $O_2$ evolution by Chlamydomonas reinhardtii UTEX 90 are inactivated, resulting in shift from aerobic to anaerobic condition. This is followed by hydrogen production catalyzed by hydrogenase. We hypothesized that the photosynthetic capacity and the accumulation of endogenous substrates such as starch for hydrogen production might be different according to cell age. Accordingly, we investigated (a) the relationships between hydrogen production, induction time of sulfur deprivation, increase of chlorophyll after sulfur deprivation, and residual PS II activity, and (b) the effect of initial cell density upon sulfur deprivation. The maximum production volume of hydrogen was 151 ml $H_2$ /l with 0.91 g/l of cell density in the late-exponential phase. We suggest that the effects of induction time and initial cell density at sulfur deprivation on hydrogen production, up to an optimal concentration, are due to an increase of chlorophyll under sulfur deprivation.


  • 주제어

    Hydrogen production .   Chlamydomonas reinhardtii .   sulfur deprivation .   cell growth stage.  

  • 참고문헌 (18)

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    3. Lee, J. H., J. S. Lee, C. S. Shin, S. C. Park, and S. W. Kim. 2000. Effects of NO and $SO_2$ on growth of highly-$CO_2$-tolerant microalgae. J. Microbiol. Biotechnol. 10: 338- 343 
    4. Melis, A., L. Zhang, M. Forestier, M. L. Ghirardi, and M. Seibert. 2000. Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol. 122: 127- 135 
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    6. Melis, A. 2002. Green alga hydrogen production: Progress, challenges and prospects. Int. J. Hydrogen Energy 27: 1217-1228 
    7. Ghirardi, M. L., L. Zhang, J. W. Lee, T. Flynn, M. Seibert, E. Greenbaum, and A. Melis. 2000. Microalgae: A green source of renewable $H_2$. Trends Biotechnol. 18: 506- 511 
    8. Wykoff, D. D., J. P. Davies, A. Melis, and A. R. Grossman. 1998. The regulation of photosynthetic electron transport during nutrient deprivation in Chlamydomonas reinhardtii. Plant Physiol. 117: 129- 139 
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    12. Lee, K. Y. and C. K. Lee. 2002. Nitrogen removal from wastewaters by microalgae without consuming organic carbon sources. J. Microbiol. Biotechnol. 12: 979- 986     
    13. Pandey, V. and L. C. Rai. 2002. Interactive effects of UV-B and pesticides on photosynthesis and nitrogen fixation of Anabaena doliolum. J. Microbiol. Biotechnol. 12: 423- 430     
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    15. Das, D. and V. Nejat. 2001. Hydrogen production by biological processes: A survey of literature. Int. J. Hydrogen Energy 26: 13- 28 
    16. Zhang, L., T. Happe, and A Melis. 2002. Biochemical and morphological characterization of sulfur-deprived and $H_{2}-producing$ Chlamydomonas reinhardtii (green alga). Planta 214: 552- 561 
    17. Rai, L. C. H. D. Kumar, F. H. Mohn, and C. J. Soeder. 2000. Services of algae to the environment. J. Microbiol. Biotechnol. 10: 119- 136 
    18. Tsygankov, A., S. Kosourov, M. Seibert, and M. L. Ghirardi. 2002. Hydrogen photoproduction under continuous illumination by sulfur-deprived, synchronous Chlamydomonas reinhardtii cultures. Int. J. Hydrogen Energy 27: 1239- 1244 
  • 이 논문을 인용한 문헌 (4)

    1. 2006. "" Journal of microbiology and biotechnology, 16(12): 1947~1953     
    2. 2006. "" Journal of microbiology and biotechnology, 16(2): 240~246     
    3. Kim, Jun-Pyo ; Sim, Sang-Jun 2006. "Effect of Limiting Factors for Hydrogen Production in Sulfur Deprived Chlamydomonas Reinhardtii" 한국수소 및 신에너지학회 논문집 = Transactions of the Korean Hydrogen and New Energy Society, 17(3): 286~292     
    4. 2006. "" Journal of microbiology and biotechnology, 16(8): 1210~1215     

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