본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of plant biotechnology v.7 no.1, 2005년, pp.27 - 35  

Identification of Different Species and Dultivars of Brassica by SDS-PAGE, Isozyme and Molecular Marker

Mukhlesur Rahman Md.    (Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Department of Genetics and Plant Breeding, Bangladesh Agricultural University   ); Hirata Yutaka    (Graduate School of Agriculture, Tokyo University of Agriculture and Technology  );
  • 초록

    Eighty-five different cultivars of Brassica rapa, B. juncea, B. nap us, B. carinata, B. oleracea and hexaploid Brassica collected from Bangladesh, Japan, China and Denmark were analyzed by SDS-PAGE for seed and leaf protein variations, using esterase, acid phosphatase and peroxidase isozyme analysis. Ten polymorphic bands were identified from seed protein however no identifiable polymorphic band was found in the leaf protein. Polymorphic markers clearly distinguished the different Brassica species as well as yellow sarson (YS) and brown seeded (BS) cultivars of B. rapa. The $F_1$ cross between YS and brown seeded cultivars showed the existance of all poly-morphic bands of the respective parents. The Bangla-deshi and Japanese cultivars of B. rapa differed in the amount of seed protein. In the case of isozyme analysis, esterase showed the highest number of polymorphic bands (13) followed by acid phosphatase (9) and peroxidase (5). These polymorphic markers were very effec-tive for classification of all the species studied in this experiment. In parentage tests using isozymes, the hybridity of intra-and-interspecific crosses of almost all the seedlings could be identified from their respective cross combinations. Esterase polymorphism showed a clear differentiation between YS and BS types of B. rapa. In addition, two esterase polymorphic markers were iden ified to differentiate some cultivars of B. juncea. Segregation patterns in these two esterase bands showed a simple Mendelian monohybrid ratio of 3:1 in $F_2$ , 1:1 in test cross and 1:0 in back cross progenies. No polymorphic band was identified to distinguish different cultivars of the same species by acid phosphatase or peroxidase. Polymerase Chain Reaction (PCR) was carried out with seed coat color specific marker of B. juncea. The yellow seeded cultivars produced a strong band at 0.5 kb and weak band 1.2 kb. In the addition of these two specific bands, Japanese yellow-seeded cultivars expressed two more weak bands at 1.0 kb and 1.1 kb. Where the brown seeded cultivars generated a single strong band at 1.1 kb. In segregating population, the yellow seed coat color marker segregated at a ratio 15 (brown) : 1 (yellow), indicating the digenic inheritance pattern of the trait.


  • 주제어

    Brassica .   SDS-PAGE .   isozymes .   PCR.  

  • 참고문헌 (19)

    1. Das S, Rajagopal J, Bhatia S, Srivastava PS, Lakshmikumaran M (2000) Assessment of genetic variation within Brassica campestris cultivars using amplified fragment length polymorphism and random amplification of polymorphic DAN markers. Plant Molecular Biology Division, Tata Energy Research Institute, Lodhi Road, New Delhi, India 
    2. Eber F, Chevre AM, Baranger A, Vallee P, Tanguy X, Renard M (1994) Spontaneous hybridization between a male sterile oilseed rape and two weeds. Theor Appl Genet 88: 362-368 
    3. Nozaki, T., Anji, M., Takahashi, T. and Ikehashi, H. (1995) Analysis of isozyme loci and their linkage in Brassica campestris L. Breeding Science 45: 57-64 
    4. Chauhan YS, Kumar K (1987) Genetics of seed color in mustard (Brassica juncea L. Czern and Coss). Cruciferae Newsl 12: 22-23 
    5. Ripley V, Thrope L, Iler S, Mizier K, Beversdorf WD (1992) Isozyme analysis as a tool for introgression of Sinapis alba germplasm into B. napus. Theor Appl Genet 84: 403-410 
    6. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA mini preparation: Version II. Plant Mol Biol Rpt 1: 19-21 
    7. Rahman MH (2001) Introgression of alleles of the isozymic locus glucose phosphatase isomerase-2 (GPI-2) from the CC genome of Brassica carinata to the CC genome of Brassica alboglabra and their independent segregation from seed color. Plant Breeding 120: 363-364 
    8. Anand IJ, Reddy WR, Rawat DS (1985) Inheritance of seed color in mustard. Indian J Genet 45: 34-37 
    9. Robbelen G, 1960. Beitrage zur Analyse des Brassica-Ge-noms. Chromosome 11: 205-228 
    10. Kumar R, Gupta VP (1985) Isozyme studies in Indian mustard (Brassica juncea L.). Theor Appl Genet. 69: 1-4 
    11. Tanksley SO, Jones RA (1986) Application of alcohol bedydrogenase allozymes in testing the genetic purity of F1 hybrids of tomato. Hort Sci 16: 179-181 
    12. Thanh VC, Hirata Y (2002) Seed storage protein diversity of three rice species in the Mekong Delta. Biosphere Conservation 4: 59-67 
    13. Chevre AM, Delourme R, Eber F, Margale E, Quiros CF, Arus P (1994) Genetic analysis and nomenclature for seven isozyme systems in Brassica nigra, B. oleracea and B. campestris. Plant Breeding 114: 473-480 
    14. Chen BY, Heneen WK, Simonsen V (1989) Comparative and genetic studies of isozymes in resynthesized and cultivated Brassica napus L., B. campestris L. and Brassica alboglabra Bailey. Theor Appl Genet 77: 673-679 
    15. Moriyuki S, Nagamine T, Terauchi T, Akamine F, Sugimoto A (1999) Isozyme application for variety identification and progeny hybridity in Japanese sugercane. Breeding Science 49: 89-95 
    16. Negi MS, Devic M, Delseny M, Lakshmikumaran M (2000) Identification of AFLP fragments linked to seed coat color in Brassica juncea and conversion to a SCAR marker for rapid selection. Theor Appl Genet 101: 146-152 
    17. Quiros CF, Ochoa O, Kianian SF, Douches D (1988) Exploring the role of x = 7 species in Brassica evolution: hybridization with B. nigra and B. oleracea. J Heredity 79: 351-358 
    18. Person K, Fait AS, von Bothmer R (2001) Genetic diversity of allozymes in turnip (Brassica rapa L. var. rapa) from the Nordic area. Hereditas 134: 43-52 
    19. Takehisa I, Noda S, Mori S, Yamashita M, Nakanishi H, Inoue M, Kamijima O (2001) Genetic variation and geographical distribution of Azuki bean (Vigna angularis) land races based on the electrophoregram of seed storage proteins. Breeding Sciences 51: 225-230 

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기