본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of plant biotechnology v.7 no.1, 2005년, pp.75 - 80  

Rapid in vitro Germination of Zygotic Embryos via Endosperm Removal in Eleutherococcus senticosus

You Xiang-Ling    (Division of Forest Resources, College of Forest Sciences, Kangwon National University   ); Choi Yong-Eui    (Division of Forest Resources, College of Forest Sciences, Kangwon National University   ); Yi Jae-Seon    (Division of Forest Resources, College of Forest Sciences, Kangwon National University  );
  • 초록

    Eleutherococcus senticosus (also called Acanthopanax senticosus), belonging to Araliaceae family, has been used as an important medicinal woody plant. Mature seeds of Eleutherococcus senticosus have rudimentary (extremely immature) zygotic embryos and require a long-term stratification for about 18 months to induce germination. Here, through the methods of endosperm removal and other exogenous treatments, we investigated the factors for inducing rudimentary embryos by in vitro culture, Rudimentary zygotic embryos in seeds were at globular to heart-shaped stage at about $250{\mu}m$ in length just after harvest of fruits. When the seeds without testa were cultured on 1/2 MS (Murashige and Skoog 1962) medium, they did not germinate regardless of medium and sucrose concentrations but the removal of endosperm tissue markedly stimulated the growth of rudimentary zygotic embryos. The embryo reached ear-lier maturation, once when the endosperm surrounding the rudimentary embryos was removed. Rudimentary zygotic embryos developed cotyledons within 3 weeks of culture after endosperm emoval. However, post-mature zygotic embryos failed to germinate though they were morphologically normal, indicating another dormancy of embryos. $GA_3\;(2.0\;\cal{mg/L})$ and/or charcoal ( $0.2\%$ ) treatment rapidly enhanced the germination of zygotic embryos. These results suggest that E. senticosus seeds have double dormancy; i. e. morphological rudimentary dormancy influenced by surrounding endosperm and physiological dormancy after post-maturation of zygotic embryos. Based on the above findings, we established the rapid germination of rudimentary zygotic embryos by in vitro culture of excised seeds with endosperm removal and $GA_3$ treatment.


  • 주제어

    Zygotic embryo .   Rudimentary embryo .   Eleuthe-rococcus senticosus .   Endosperm removal .   Morphological and physiological dormancy.  

  • 참고문헌 (18)

    1. Brekham II (1960) A new medicinal plant of the family Araliaceae-the spiny ELetttherococcus. Izv Sibir Otdel Akad Nauk USSR 9: 113-120 
    2. Choi YE, Kim JW, Yoon ES (1999) High frequency of plant production via somatic embryogenesis from callus or cell suspension cultures in Eleutherococcus senticosus. Ann Bot 83: 309-314 
    3. Hepher A, Roberts JA (1985a) The control of seed germination in Trollius Ledebouri: The breaking of dormancy. Planta 166: 314-320 
    4. Liu LD, Wang ZL, Tian GW and Shen JH (1998) The development of embryo and endosperm in Eletherococcus senticosus (Araliaceae). Acta Phytotaxonomica Sinica 36: 298-304 
    5. Park HK, Park MS, Kim TS, Kim S, Choi KG, Park KH (1997) Characteristics of embryo growth and dehiscence during the after-ripening period in Eleutherococcus senticosus. Korean J Crop Sci 42: 637-677 
    6. Wang PJ and Huang LC (1976) Beneficial effects of activated charcoal on plant tissue and organ culture. In Vitro 12: 260-262 
    7. Zhu N, Wang YH (1992) Reproductive ecological studies of Acanthopanax senticosus (II)---Seed dispersal, seed bank and recruitment. Journal of Northeast Forestry University 20: 13-17 
    8. Hu CY (1976) Light-mediated inhibition of in vitro development of rudimentary embryos of llex opaca. Amer J Bot 63: 651-656 
    9. Hepher A, Roberts JA (1985b) The control of seed germination in Trollius Ledebouri: The model of dormancy. Planta 166: 321-328 
    10. Hu CY (1975) In vitro culture of rudimentary embryos of eleven llex species. J Amer Soc Horti Sci 100: 221-225 
    11. Hudson T, Hartmann, Dale E, Kester, Fred E. Davies (1990) Plant propagation-principles and practices. Prentice Hall Career and Technology Englewood Cliffs, New Jersey pp 114 
    12. Fridborg G, Pedersen M, Landsrom, Eriksson T (1978) The effect of activated charcoal on tissue cultures: Absorption of metabolites inhibiting morphogenesis. Physiol Plant 43: 104-106 
    13. Linsmaier EM and Skoog F (1983). Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100-127 
    14. Sansberro PA, Rey HY, Mrogiski LA and Collavino MM (1998) In Vitro culture of rudimentary embryos of llex paraguariensis: responses to exogenous cytokinins. J Plant Growth Regul 17: 101-105 
    15. Zhao Y, Liu H, Liu T, Fu J, Liu W, Zhang X (2000) Affection of exogenous gibberellic acid ($GA_3$) on endogenous hormones of Panax quinquefolium seed during its morphological after-ripening period. Zhong Yao Cai 23: 593 
    16. Bewley JD (1997) Seed germination and dormancy. Plant Cell 9: 1055-1066 
    17. Isoda S, Shoji J (1994) Studies on the cultivation of Eleutherococcus senticosus Maxim. II On the germination and raising of seedling. Natural Medicine 48: 75-81 
    18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-479 

 저자의 다른 논문

  • Choi, Yong-Eui (38)

    1. 2004 "지황 부정근을 이용한 식물체 재분화 및 생물반응기 배양" 식물생명공학회지 = Korean journal of plant biotechnology 31 (1): 55~60    
    2. 2004 "현탁배양을 통한 금낭화(Dicentra spectabilis L. Lemaire)의 대량증식" 식물생명공학회지 = Korean journal of plant biotechnology 31 (2): 121~126    
    3. 2004 "구기자나무 (Lycium chinense)의 모상근 유도 및 생물반응기 배양" 식물생명공학회지 = Korean journal of plant biotechnology 31 (4): 295~300    
    4. 2005 "Cloning and Characterization of PMET3a from Populus alba${\times}$Populus glandulosa" Plant resources 8 (1): 1~8    
    5. 2005 "Molecular Characterization and Expression of CuZn-superoxide Dismutase (PSOD1) from Populus alba${\times}$Populus glandulosa" Plant resources 8 (1): 52~59    
    6. 2005 "Authentication of Korean Panax ginseng from Chinease Panax ginseng and Panax quinquefolius by AFLP analysis" Journal of plant biotechnology 7 (2): 81~86    
    7. 2005 "Enhanced Biosynthesis of ${\alpha}$-tocopherol in Transgenic Soybean by Introducing ${\gamma}$-TMT gene" Journal of plant biotechnology 7 (3): 203~209    
    8. 2005 "우량묘삼 대량생산 기술현황 및 향후 연구방향" 인삼·약초 3 (4): 114~121    
    9. 2006 "Optimization of Direct Somatic Embryogenesis from Mature Zygotic Embryos of Panax ginseng C.A. Meyer" Journal of plant biology = 식물학회지 49 (5): 348~352    
    10. 2006 "Adventitious Root Development and Ginsenoside Production in Panax ginseng, Panax quinquefolium and Panax japonicum" Journal of plant biotechnology = 식물생명공학회지 33 (2): 147~152    
  • Yi, Jae-Seon (53)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기