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Biotechnology and bioprocess engineering v.10 no.2, 2005년, pp.109 - 114   피인용횟수: 2

[ $Ce^{4+}$ ]-Stimulated Ion Fluxes Are Responsible for Apoptosis and Taxol Biosynthesis in Suspension Cultures of Taxus Cells

Li Jing-Chuan    (Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University   ); Ge Zhi-Qiang    (Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University   ); Yuan Ying-Jin    (Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University  );
  • 초록

    Ion fluxes across the plasma membrane activated by 1 mM $Ce^{4+}$ , cell apoptosis and taxol biosynthesis in suspension cultures of Taxus cusp/data were studied. The extracellular pH sharply decreased upon the addition of 1 mM $Ce^{4+}$ , then increased gradually and exceeded the initial pH value over a time period of 12 h. The extracellular $Ca^{2+}$ concentration decreased within the first 3 h after the addition of $Ce^{4+}$ , then gradually decreased to one third of initial value in control at about 72 h and remained unchanged afterwards. Experiments with an ion channel blocker and a $Ca^{2+}$ -channel blocker indicated that the dynamic changes in extracellular pH and the $Ca^{2+}$ concentration resulted from the $Ce^{4+}$ -induced activation of W uptake and $Ca^{2+}$ influx across the plasma membrane via ion channels. A pretreatment of the ion channel blocker initiated $Ce^{4+}$ -treated cells to undergo necrosis, and the prior addition of the $Ca^{2+}$ -channel blocker inhibited $Ce^{4+}$ -induced taxol biosynthesis and apoptosis. It is thus inferred that W uptake is necessary for cells to survive a $Ce^{4+}$ -caused acidic environment and is one of the mechanisms of $Ce^{4+}$ -induced apoptosis. Furthermore, the $Ca^{2+}$ influx across the plasma membrane mediated both the $Ce^{4+}$ -induced apoptosis and taxol biosynthesis.


  • 주제어

    apoptosis .   ion fluxes .   taxus cells.  

  • 참고문헌 (27)

    1. Hirasuna, T. J., L. J. Pestchanker, and V. Srinivasan (1996) Taxol production on suspension cultures of Taxus baccata. Plant Cell Tiss. Org. Cult. 44: 95-106 
    2. Yuan, Y. J., Z. Y. Ma, Z. Q. Ge, Z. Q. Miao, and A. P. Zeng (2000) Apoptosis and taxol production in suspension cultures of Taxus spp. cells. J. Chem. Ind. Eng. 51(Suppl.): 43-46 
    3. Serrano, R. (1989) Structure and function of plasma membrane ATPase. Annu. Rev. Plant Physiol. Plant Mol. Biol. 40: 61-94ne4 
    4. Gelli, A., V. J. Higgins, and E. Blumwald (1997) Activation of plasma membrane $Ca^{2+}$-permeable channels by race-specific fungal elicitors. Plant Physiol. 113: 269?279 
    5. Sze, H., X. Li, and M. G. Palmgren (1999) Energization of plant cell membranes by $H^+$- pumping ATPases: Regulation and biosynthesis. Plant Cell 11: 677-689 
    6. Lichtenthaler, H. K. (1998) The stress concept in plants: An introduction. Ann. N. Y. Acad. Sci. 351: 187-198 
    7. Li, J. and A. Eastman (1995) Apoptosis in a interleukin-2- dependent cytotoxic T lymphocyte cell line is associated with intracellular acidification. J. Biol. Chem. 270: 3203-3211 
    8. Rajotte, D., P. Haddad, A. Haman, E. J. J. Cragoe, and T. Hoang (1992) Role of protein kinase C and the $Na^+/H^+$ antiport in suppression of apoptosis by granulocyte macrophage colony-stimulating factor in interleukin-3. J. Biol. Chem. 267: 9980-9987 
    9. Yuan, Y. J., G. W. Hu, and C. G. Wang (1998) Effect of La, Ce on Taxus cuspidata cell growth, biosynthesis and release of taxol. J. Rare Earth 16: 300-306 
    10. Baker, C. J., M. M. Atkinson, and A. Collmer (1987) Concurrent loss in Tn5 mutants of Pseudomonas syringae pv. syringae of the ability to induce the hypersensitive response and host plasma membrane $K^+/H^+$ exchange in tobacco. Phytopathology 77: 1268-1272 
    11. Segal, J. (1986) Lanthanum increases the rat thymocytes cytoplasmic free calcium concentration by enhancing calcium influx. Biochim. Biophys. Acta 886: 267-271 
    12. Ormerod, M. G., X. M. Sun, R. T. Snowden, and R. Davies (1993) Increased membrane permeability of apoptotic thymocytes: A flow cytometric study. Cytometry 14: 595-602 
    13. Atkinson, M. M., L. D. Keppler, and E. W. Orlandi (1990) Involvement of plasma membrane calcium influx in bacterial induction of the $K^+/H^+$ and hypersensitive responses in tobacco. Plant Physiol. 92: 215-221 
    14. Hu, G. W., J. C. Li, and Y. J. Yuan (2000) Effect of rare earth on Taxus cuspidata intracellular calcium concentration. J. Tianjin Uni. (Sci. Technol.) 33:19-20 
    15. Blein, J. P., M. L. Milat, and P. Ricci (1991) Responses of cultured tobacco cells to cryptogein, a proteinaceous elicitor form Phytophthora cryptogea. Plant Physiol. 95: 486-491 
    16. Yukimune, Y., H. Tabata, Y. Higashi, and Y. Hara (1996) Methyl jasmonic-induced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures-addition of methyl jasmonic acid as elicitor to Taxus baccata and Taxus medis suspension cell culture. Natural Biotech. 14: 1129-1132 
    17. Xu, H. and M. C. Heath (1998) Role of calcium in signal transduction during the hypersensitive response caused by basidiospore-derived infection of the Cowpea rust fungus. Plant Cell 10: 585-598 
    18. Wu, Z. L., Y. J. Yuan, Z. H. Ma, and Z. D. Hu (2000) Kinetics of two-liquid-phase Taxus cuspidata cell culture for production of Taxol. Biochem. Eng. J. 5: 137-142 
    19. Nurnberger, T., D. Nennstiel, T. Jabs, W. R. Sacks, K. Hahlbrock, and D. Scheel (1994) High affinity binding of a fungal oligopeptide elicitor to parsley plasma membranes triggers multiple defense responses. Cell 78: 449-460 
    20. Yuan, Y. J., J. C. Li, Z. Q. Ge, and J. C. Wu (2002) Superoxide anion burst and taxol production induced by $Ce^{4+}$ in suspension cultures of Taxus cuspidata. J. Mol. Catalysis B: Enzymatic 18: 551-560 
    21. Barry, M. A., J. E. Roynolds, and A. Eastman (1993) Etopside-induced apoptosis in human HL-60 cells is associated with intracellular acidification. Cancer Res. 53: 2349-2357 
    22. Yuan, Y. J., Z. Q. Ge, J. C. Li, J. C. Wu, and Z. D. Hu (2002) Differentiation of apoptotic and necrotic cells in suspension cultures of Taxus cuspidata by the combined use of fluorescent dying and histochemical staining methods. Biotechnol. Lett. 24: 71-76 
    23. Li, J. C., Z. H. Ma, Y. J. Yuan, A. C. Sun, and C. X. Hu (2001) Dynamic effects of cerium on syntheses of soluble protein and taxol in suspension culture of Taxus Chinensis var. mairei cells. Rare Earth 19: 233-238 
    24. Powis, D. A., C. L. Clark, and K. J. O'brien (1994) Lanthanum can be transported by the sodium-calcium exchange pathway and directly triggers cathcholamine release from bovine chromaffin cells. Cell Calcium 16: 377-390 
    25. Ketchum, R. E. B., D. M. Gibson, R. B. Croteau, and M. L. Shuler (1999) The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol. Bioeng. 62: 97-105 
    26. Chen, Q., R. S. P. Benson, A. D. Whetton, S. R. Brant, M. Donowitz, M. H. Montrose, C. Dive, and A. J. M. Watson (1997) Role of acid/base homeostasis in the suppression of apoptosis in haemopoietic cells by v-Abl protein tyrosine kinase. J. Cell Sci. 110: 379-387 
    27. Jabs, T., M. Tschope, C. Colling, K. Hahlbrock, and D. Scheel (1997) Elicitor-stimulated ion fluxes and $O_2^-$ from the oxidative burst are essential components in triggering defense gene activation and phytoalexins synthesis in parsley. Proc. Natl. Acad. Sci. USA 94: 4800-4805 
  • 이 논문을 인용한 문헌 (2)

    1. 2007. "" Biotechnology and bioprocess engineering, 12(4): 354~358     
    2. 2007. "" Biotechnology and bioprocess engineering, 12(6): 653~661     

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