본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Biotechnology and bioprocess engineering v.10 no.2, 2005년, pp.115 - 121   피인용횟수: 7

Observed Quasi-steady Kinetics of Yeast Cell Growth and Ethanol Formation under Very High Gravity Fermentation Condition

Chen Li-Jie    (Department of Bioscience and Bioengineering, Dalian University of Technology   ); Xu Ya-Li    (Department of Chemical Engineering, University of Waterloo   ); Bai Feng-Wu    (Department of Bioscience and Bioengineering, Dalian University of Technology, Department of Chemical Engineering, University of Waterloo   ); Anderson William A.    (Department of Chemical Engineering, University of Waterloo   ); Murray Moo-Young    (Department of Chemical Engineering, University of Waterloo  );
  • 초록

    Using a general Saccharomyces cerevisiae as a model strain, continuous ethanol fermentation was carried out in a stirred tank bioreactor with a working volume of 1,500 mL. Three different gravity media containing glucose of 120, 200 and 280 g/L, respectively, supplemented with 5 g/L yeast extract and 3 g/L peptone, were fed into the fermentor at different dilution rates. Although complete steady states developed for low gravity medium containing 120 g/L glucose, quasi-steady states and oscillations of the fermented parameters, including residual glucose, ethanol and biomass were observed when high gravity medium containing 200 g/L glucose and very high gravity medium containing 280 g/L glucose were fed at the designated dilution rate of $0.027\;h^{-1}$ . The observed quasi-steady states that incorporated these steady states, quasi-steady states and oscillations were proposed as these oscillations were of relatively short periods of time and their averages fluctuated up and down almost symmetrically. The continuous kinetic models that combined both the substrate and product inhibitions were developed and correlated for these observed quasi-steady states.


  • 주제어

    Saccharomyces cerevisiae .   continuous ethanol fermentation .   quasi-steady states .   oscillations .   observed quasi-steady states .   kinetics.  

  • 참고문헌 (26)

    1. Aiba, S., M. Shoda, and M. Nagatani (2000) Kinetics of product inhibition in alcohol fermentation. Biotechnol. Bioeng. 67: 671-690 
    2. Bazua, C. D. and C. R. Wilke (1977) Ethanol effects on the kinetics of a continuous fermentation with Saccharomyces cerevisiae. Biotechnol. Bioeng. Symp. 7: 105-108 
    3. Caro, I., L. Perez, and D. Cantero (1991) Development of a kinetic model for the alcoholic fermentation of must. Biotechnol. Bioeng. 38: 742-748 
    4. Bafrncova, P., D. Smogrovicova, I. Slavikova, J. Patkoa, and Z. Domeny (1999) Improvement of very high gravity ethanol fermentation by media supplementation using Saccharomyces cerevisiae. Biotechnol. Lett. 21: 337-341 
    5. Thomas, K. C., S. H. Hynes, and W. M. Ingledew (1996) Practical and theorectical considerations in the production of high concentrations of alcohol by fermentation. Process Biochem. 31: 321-331 
    6. Levenspiel, O. (1980) The Monod equation: A revisit and generalization to product inhibition situations. Biotechnol. Bioeng. 22: 1671-1687 
    7. Thatipamala, R., S. Rohani, and G. A. Hill (1992) Effects of high product and substrate inhibitions on the kinetics and biomass and product yields during ethanol batch fermentation. Biotechnol. Bioeng. 40: 289-297 
    8. Kelsall, D. R. and T. P. Lyons (1999) Management of fermentations in the production of alcohol: Moving towards 23% ethanol. pp. 26-38. In: K. A. Jacques, T. P. Lyons, and D. R. Kelsall (eds.). The Alcohol Textbook: A Reference for the Beverage, Fuel and Industrial Alcohol Industries. Nottingham University Press, UK 
    9. Converti, A., P. Perego, A. Lodi, F. Parisi, and M. D. Borghi (1985) A kinetic study of Saccharomyces strains: Performance at high sugar concentrations. Biotechnol. Bioeng. 27: 1108-1115 
    10. Ghose, T. K. and R. D. Tyagi (1979) Rapid ethanol fermentation of cellulose hydrolysate II. Product and substrate inhibition and optimization of fermentor design. Biotechnol. Bioeng. 21: 1401-1420 
    11. Bai, F. W., L. J. Chen, W. A. Anderson, and M. Moo-Young (2004) Parameter oscillations in very high gravity medium continuous ethanol fermentation and their attenuation on multi-stage packed column bioreactor system. Biotechnol. Bioeng. 88: 558-566 
    12. Edwards, V. H. (1970) The influence of high substrate concentrations on microbial kinetics. Biotechnol. Bioeng. 12: 679-712 
    13. Converti, A., P. Perego, M. D. Borghi, F. Parisi, and G. Ferraiolo (1986) Kinetic considerations about the study of alcoholic fermentations of starch hydrolysate. Biotechnol. Bioeng. 28: 711-717 
    14. Borzani, W. (2001) Variation of the ethanol yield during oscillatory concentrations changes in undisturbed continuous ethanol fermentation of sugar-cane blackstrap molasses. World J. Microbiol. Biotechnol. 17: 253-258 
    15. Dourado, A., A. D. Calvet, Y. Sevely, and G. Goma (1987) Modeling and static optimization of the ethanol production in a cascade reactor II static optimization. Biotechnol. Bioeng. 29: 195-203 
    16. Dourado, A., G. Goma, U. Albuquerque, and Y. Sevely (1987) Modeling and static optimization of the ethanol production in a cascade reactor I modeling. Biotechnol. Bioeng. 29: 187-194 
    17. Luong, J. H. T. (1985) Kinetics of ethanol inhibition in alcohol fermentation. Biotechnol. Bioeng. 27: 280-285 
    18. Bayrock, D. P. and W. M. Ingledew (2001) Application of multistage continuous fermentation for production of fuel alcohol by very-high-gravity fermentation technology. J. Indus. Microbiol. Technol. 27: 87-93 
    19. Chattaway, T. and G. Goma (1988) Modelling ethanol and secondary inhibitions of ethanol fermentation in a multistage reactor. Biotechnol. Bioeng. 32: 271-276 
    20. Warren, R. K., G. A. Hill, and D. G. Macdonald (1990) Improved bioreaction kinetics for simulation of continuous ethanol fermentation by Saccharomyces cerevisiae. Biotechnol. Prog. 6: 319-325 
    21. Bai, F. W., L. J. Chen, Z. Zhang, W. A. Anderson, and M. Moo-Young (2004) Continuous ethanol production and evaluations of yeast cell lysis and viability-loss under very high gravity medium condition. J. Biotechnol. 110: 287-293 
    22. Bovee, J. P., P. Strehaiano, G. Goma, and Y. Sevely (1984) Alcoholic fermentation: Modeling based on sole substrate and product measurement. Biotechnol. Bioeng. 26: 328-334 
    23. Borzani, W. (1987) Kinetics of ethanol production during the reactor feeding phase in constant fed-batch fermentation of molasses. Biotechnol. Bioeng. 29: 844-849 
    24. Andrews, J. F. (1968) A mathematical model for the continuous culture of microorganisms utilizing inhibitory substrates. Biotechnol. Bioeng. 10: 707-723 
    25. Lin, Y. H., D. Bayrock, and W. M. Ingledew (2001) Metabolic flux variation of Saccharomyces cerevisiae cultivated in a multistage continuous stirred tank reactor fermentation environment. Biotechnol. Prog. 17: 1055-1060 
    26. Holzberg, I., R. K. Finn, and K. H. Steinkraus (1967) A kinetic study of the alcoholic fermentation of grape juice. Biotechnol. Bioeng. 9: 413-427 
  • 이 논문을 인용한 문헌 (7)

    1. 2007. "" Biotechnology and bioprocess engineering, 12(5): 566~573     
    2. 2007. "" Biotechnology and bioprocess engineering, 12(6): 690~695     
    3. 2008. "" Biotechnology and bioprocess engineering, 13(6): 765~771     
    4. 2009. "" Biotechnology and bioprocess engineering, 14(5): 591~598     
    5. 2009. "" Biotechnology and bioprocess engineering, 14(5): 606~611     
    6. 2009. "" Biotechnology and bioprocess engineering, 14(4): 496~502     
    7. Jeon, Hyung-Jin ; Lee, Byung-Oh ; Kang, Kyung-Woo ; Jeong, Jun-Seong ; Chung, Bong-Woo ; Choi, Gi-Wook 2011. "Production of Bioethanol by Using Beverage Waste" KSBB Journal, 26(5): 417~421     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기