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Listeria 속균 신속 검출을 위한 면역크로마토그라피 킷트 개발
Development of the immunochromatographic strip for the rapid detection of Listeria spp.

정병열    (농림부 국립수의과학검역원 동물약품과   ); 정석찬    (농림부 국립수의과학검역원 축산물규격과   ); 김종만    (농림부 국립수의과학검역원 세균과  );
  • 초록

    We developed the one-step strip based on an immunochromatographic (IC) assay for the rapid detection of Listeria spp. Genus-specific monoclonal antibody to flagella of L. monocytogenes was conjugated with 40 nm colloidal gold particles which were prepared in our laboratory. The specificity of the IC strip was tested with pure cultured bacteria. All strains of the genus of Listeria spp. yielded positive reactions and 12 strains of non-Listeria were negative, resulting in a specificity of 100%. L. monocytogenes was artificially inoculated in raw pork macerated with listeria enrichment broth. And then it was 10-fold diluted from $8.7{\times}10^6$ to 8.7 CFU/ml. L. monocytogenes could be detected at a minimum of $8.7{\times}10^5CFU/ml$ before enrichment, $8.7{\times}10^2CFU/ml$ after primary enrichment and 8.7 CFU/ml after secondary enrichment, respectively. These results indicated that the IC strip exhibited high specificity and sensitivity in the detection of Listeria spp.


  • 주제어

    Listeria .   immunochromatographic strip .   colloidal gold.  

  • 참고문헌 (17)

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