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Potentiation of Ceramide-Induced Apoptosis by $p27^{kip1}$ Overexpression

Kim Hae Jong    (Division of Biochemistry, College of Pharmacy, Chung-Ang University   ); Ghil Kyung Chul    (Division of Biochemistry, College of Pharmacy, Chung-Ang University   ); Kim Moo Sung    (Division of Biochemistry, College of Pharmacy, Chung-Ang University   ); Yeo Seong Hyun    (Division of Biochemistry, College of Pharmacy, Chung-Ang University   ); Chun Young Jin    (Division of Biochemistry, College of Pharmacy, Chung-Ang University   ); Kim Mie Young    (Division of Biochemistry, College of Pharmacy, Chung-Ang University  );
  • 초록

    The cyclin-dependent kinase inhibitor $p27^{kip1}$ (p27) has been implicated in the regulation of cell cycle and apoptosis. Recently, we have demonstrated that ceramide induces apoptotic cell death associated with increase in the level of p27 in HL-60 cells. In the present study, we showed that overexpression of p27 increases ceramide-induced apoptotic cell death in HL-60 cells. Furthermore, overexpression of p27 accelerated DNA fragmentation, PARP cleavage and cytochrome c release induced by ceramide. In addition, ceramide induced Sax expression independent of p27. These findings indicate that enhanced effect on apoptosis by p27 is associated with mitochondrial signaling which involves cytochrome c release.


  • 주제어

    Ceramide .   p27 .   Apoptosis .   Cytochrome c release.  

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    2. 2003 "Effects of a Naphthoquinone Analog on Tumor Growth and Apoptosis Induction" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 26 (5): 405~410    
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  • Kim, Mie-Young (11)

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