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Effect of Low Adapted Temperature and Medium Composition on Growth and Erythropoietin (EPO) Production by Chinese Hamster Ovary Cells

Kim Na Young    (College of Pharmacy, Chung Ang University   ); Kim Jung Hoe    (Department of Biological Sciences, KAIST   ); Kim Hong Jin    (College of Pharmacy, Chung Ang University  );
  • 초록

    Temperature and medium composition were changed with the aim of increasing growth and erythropoietin (EPO) production in EPO-producing Chinese hamster ovary (CHO) cells. We used the CHO cell line, IBE, and its derivative, CO5, which over-expresses the first two enzymes of the urea cycle, carbamoyl phosphate synthetase I (CPS I) and ornithine transcar-bamoylase (OTC). When supplements were added to the medium at $33\;^{\circ}C$ , the growth of IBE and CO5 cells increased by $27\%\;and;26\%$ , respectively and the maximum yield of EPO was increased by $40\%$ in both cell lines. The absolute EPO concentration in the CO5 cells was always $55{\sim}60\%$ higher than in the IBE cells. In addition, when the two cell lines were continuously cultured with supplements at $33\;^{\circ}C$ until their growth rates approached those at $37\;^{\circ}C$ , the growth rates of both IBE and CO5 cells increased by $54\%$ and their maximum EPO levels increased by up to $73\%\;and\;56\%$ , respectively. Therefore, the growth and EPO expression levels of CO5 cells increased 2.2-fold and 2.6-fold, respectively, compared to those of the IBE cells. These results indicate that adaptation to lower temperature as well as medium supplementation could be important for improving cell growth and EPO production.


  • 주제어

    CHO cells .   erythropoietin (EPO) .   Temperature .   Serum-free medium .   Supplement.  

  • 참고문헌 (25)

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  • 이 논문을 인용한 문헌 (2)

    1. 2009. "" Biotechnology and bioprocess engineering, 14(4): 406~413     
    2. Koo, Bon Chul ; Kwon, Mo Sun ; Kim, Teoan 2016. "In Vitro Assay of Mammary Gland Tissue Specific hEPO Gene Expression" Reproductive & developmental biology = 한국동물번식학회지, 40(1): 7~13     

 저자의 다른 논문

  • 김나영 (2)

    1. 2004 "Enhancement of Erythropoietin Production from Chinese Hamster Ovary(CHO) Cells by Introduction of the Urea Cycle Enzymes, Carbamoyl Phosphate Synthetase I and Ornithine Transcarbamylase" Journal of microbiology and biotechnology 14 (4): 844~851    
    2. 2007 "Carbamoyl Phosphate Synthetase I이 요소회로 유전자를 발현하는 CHO 세포 주의 세포 성장과 재조합 Erythropoietin의 생산에 미치는 영향" 약학회지 = Yakhak hoeji 51 (3): 214~218    

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