본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Benzodiazepine System is Involved in Hyperalgesia in Rats Induced by the Exposure to Extremely Low Frequency Magnetic Fields

Jeong Ji Hoon    (Department of Pharmacology, College of Pharmacy, Chung Ang University   ); Choi Kyung Bum    (Department of Pharmacology, College of Pharmacy, Chung Ang University   ); Moon Nam Ju    (Department of Ophthalmology, Chung Ang University   ); Park Eon Sub    (Department of Pathology, College of Medicine, Chung Ang University   ); Sohn Uy Dong    (Department of Pharmacology, College of Pharmacy, Chung Ang University  );
  • 초록

    Many reports demonstrate that extremely low frequency magnetic fields (ELF MFs, 60 Hz) may be involved in hyperalgesia. In a previous investigation, we suggested that MFs may produce hyperalgesia and such a response may be regulated by the benzodiazepine system. In order to further confirm this effect of MFs, we used diazepam and/or flumazenil with MFs exposure. When testing the pain threshold of rats using hot plate tests, MFs or diazepam ( $0.5\;{\mu}g$ , i.c.v.; a benzodiazepine receptor agonist) induced hyperalgesic effects with the reduction of latency. These effects were blocked by a pretreatment of flumazenil (1.5 mg/kg, i.p.; a benzodiazepine receptor antagonist). When the rats were exposed simultaneously to MFs and diazepam, the latency tended to decrease without statistical significance. The induction of hyperalgesia by co-exposure to MFs and diazepam was also blocked by flumazenil. However, the pretreatment of GABA receptor antagonists such as bicuculline ( $0.1\;{\mu}g$ , i.c.v.; a $GABA_A$ antagonist) or phaclofen ( $10\;{\mu}g$ , i.c.v.; a $GABA_B$ antagonist) did not antagonize the hyperalgesic effect of MFs. These results suggest that the benzodiazepine system may be involved in MFs-induced hyperalgesia.


  • 주제어

    Magnetic fields .   Hyperalgesia .   Diazepam .   Flumazenil .   GABA.  

  • 참고문헌 (23)

    1. Bowery, N. G., $GABA_{B}$ receptors and their significance in mammalian pharmacology. Trends Pharmacol. Sci., 10, 401- 407 (1989) 
    2. Frey, A. H., Electromagnetic field interactions with biological systems. FASEB J., 7, 272-281 (1993) 
    3. Gould, J. L., Magnetic field sensitivity in animals. Annu. Rev. Physiol., 46, 585-598. (1984) 
    4. Hillert L., Kolmodin-Hedman B., Hypersensitivity to electricity: sense or sensibility? J. Psychosom. Res., 42, 427-432 (1997) 
    5. Kavaliers, M., Ossenkopp, K. P., and Hirst, M., Magnetic fields abolish the enhanced nocturnal analgesic response to morphine in mice. Physiol. Behav., 32, 261-264 (1984) 
    6. Mantegazza, P., Parenti, M., Tammiso, R., Vita, P., Zambotti, F., and Zonta, N., Modification of the antinociceptive effect of morphine by centrally administered diazepam and midazolam. Br. J. Pharmacol., 75, 569-572 (1982) 
    7. Matsumoto, R., GABA receptors: are cellular differences reflected in function. Brain Res. Rev., 14, 203-225 (1989) 
    8. Ossenkopp, K. P., Kavaliers, M., Prato, F. S., Teskey, G. C., Sestini, E., and Hirst, M., Exposure to nuclear magnetic resonance imaging procedure attenuates morphine-induced analgesia in mice. Life Sci., 37, 1507-1514 (1985) 
    9. Papi, F., Ghione, S., Rosa, C., del Seppia, C., and Luschi, P., Exposure to oscillating magnetic fields influences sensitivity to electrical stimuli. II. Experiments on humans. Bioelectromagnetics, 16, 295-300 (1995) 
    10. Paxinos, G. and Watson, C., The rat brain in stereotaxic coordinates. 2nd edn., Academic Press, San Diego, (1986) 
    11. Rosland, J. H., Hunskaar, S., and Hole, K., Diazepam attenuates morphine antinociception test-dependently in mice. Pharmacol. Toxicol., 66, 382-386 (1990) 
    12. Costa, E. and Guidotti, A., Molecular mechanisms in the receptor action of benzodiazepines. Annu. Rev. Pharmacol., 19, 531-545 (1979) 
    13. Kavaliers, M., Choleris, E., Prato, F. S., and Ossenkopp, E., Evidence for the involvement of nitric oxide and nitric oxide synthase in the modulation of opioid-induced antinociception and the inhibitory effects of exposure to 60 Hz magnetic fields in the land snail. Brain Res., 809, 50-57 (1998) 
    14. Tatsuo, M. A., Salgado, J. V., Yokoro, C. M., and Duarte, I. D., and Francischi, J. N., Midazolam-induced hyperalgesia in rats: modulation via $GABA_{A}$ receptors at supraspinal level. Eur. J. Pharmacol., 370, 9-15 (1999) 
    15. Luger, T. J., Hayashi, T., Lorenz, I. H., and Hill, H. F., Mechanism of the influence of midazolam on morphine antinociception at spinal and supraspinal levels in rats. Eur. J. Pharmacol., 271, 421-431 (1994) 
    16. Jeong, J. H., Choi, K. B., Yi, B. C., Chun, C. H., Sung, K. Y., Sung, J. Y., Kim, J. H., Gimm, Y. M., Huh, I. H., and Sohn, U. D., Effects of extremely low frequency magnetic fields on pain thresholds in mice: roles of melatonin and opioids. J. Auton. Pharmacol., 20, 259-264 (2000) 
    17. Tallman, J. and Gallaher, D., The GABAergic system: a locus of benzodiazepine action. Annu. Rev. Neurosci., 8, 21-24 (1985) 
    18. Adey, W. R., Tissue interactions with nonionizing electromagnetic fields. Physiol. Rev., 61, 435-514 (1981) 
    19. Kavaliers, M. and Ossenkopp, K. P., Magnetic field inhibition of morphine-induced analgesia and behavioral activity in mice: evidence for involvement of calcium ions. Brain Res., 379, 30-38 (1986) 
    20. Ossenkopp, K. P. and Ossenkopp, M. D., Geophysical variables and behavior: XI. Open-field behaviors in young rats exposed to an elf rotating magnetic field. Psychol. Rep., 52, 343-349 (1983) 
    21. Enna, S. J. and Karbon, E. W., GABA receptors: an overview, In Olson R. W., and Venter J. C. (Eds.). Benzodiazepine/GABA Receptors and Chloride Channels: Structural and Functional Properties. Liss, New York, pp. 41, (1986) 
    22. Niv, D., Davidovich, S., Geller, E., and Urca, G., Analgesic and hyperalgesic effects of midazolam: dependence on route of administration. Anesth. Anag., 7, 1169-1173 (1988) 
    23. Bormann, J., Electrophysiology of $GABA_{A}$ and $GABA_{B}$ receptor subtypes. Trends Neurosci., 11, 112-116 (1988) 

 저자의 다른 논문

  • 정지훈 (31)

    1. 2000 "Differential Effects of Nitric Oxide Synthase Inhibitors in Rats" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 4 (2): 99~104    
    2. 2000 "Omeprazole-cholestyramine resin 제제의 위산분비에 대한 억제효과" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 8 (4): 318~324    
    3. 2003 "The Role of Ascorbic Acid on the Redox Status and the Concentration of Malondialdehyde in Streptozotocin-Induced Diabetic Rats" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 26 (3): 237~243    
    4. 2003 "The Influences of Extremely Low Frequency Magnetic Fields on Drug-Induced Convulsion in Mouse" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 26 (6): 487~492    
    5. 2004 "MAPK Activation and Cell Viability after $H_2O_2$ Stimulation in Cultured Feline Ileal Smooth Muscle Cells" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 8 (6): 339~344    
    6. 2004 "한국인에서의 경구용 클로나제팜의 약물동력학적 특성" 약학회지 = Yakhak hoeji 48 (5): 278~284    
    7. 2004 "극저주파 자기장으로 유도한 생쥐의 통각과민에 $\alpha_2$-수용체의 관련성" 약학회지 = Yakhak hoeji 48 (5): 285~290    
    8. 2004 "흰쥐의 염증반응에 대한 CW-501027과 CW-501029의 억제효과" 약학회지 = Yakhak hoeji 48 (6): 317~322    
    9. 2005 "Hepatoprotective Effects of Ginseng Intestinal Metabolite IH-901 on Chemical-Induced Hepatic Damage" Food science and biotechnology 14 (4): 558~560    
    10. 2005 "Extremely Low Frequency Magnetic Fields Modulate Bicuculline-Induced-Convulsion in Rats" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 28 (5): 587~591    
  • 최경범 (4)

  • 박언섭 (15)

  • 손의동 (85)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기