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The Korean journal of parasitology v.43 no.1 = no.133, 2005년, pp.15 - 18   피인용횟수: 1

Diagnostic value of a dot immunobinding assay for human pulmonary hydatidosis

OLUT Ali I.    (Department of Infectious Diseases, Tepecik SSK Training Hospital   ); ERGUVEN Sibel    (Department of Parasitology and Hacettepe University Hospital   ); EMRI Salih    (Department of Chest Diseases, Hacettepe University Hospital   ); OZUNLU Haluk    (Department of Infectious Diseases, Tepecik SSK Training Hospital   ); AKAY Hadi    (Department of Chest Surgery, Ankara University Ibni Sina Hospital  );
  • 초록

    The diagnosis of human hydatidosis is primarily made using radiological and serological methods. Radiological methods are generally of low specificity and serological methods lack sensitivity, especially for pulmonary disease. In this study the capabilities of a new rapid test, the hydatid antigen dot immunobinding assay (HA-DIA), which was developed for the diagnosis of pulmonary hydatidosis, were studied and compared with another immunodiagnostic method, indirect hemagglutination (IHA). The study subjects included 18 patients, 9 women, 9 men; range 7 to 63 years; mean 30 years, with surgically proven pulmonary hydatidosis, a control group comprised of 14 patients; viral respiratory infections (1), cirrhosis (2), connective tissue disease (2), taeniasis (3), and 6 healthy donors. We found that the HA-DIA test had a sensitivity of $67\%$ and specificity of $100\%$ , and that the IHA test had a sensitivity of $50\%$ and specificity of $100\%$ . We conclude that HA-DIA is a simple, rapid, low cost assay that does not require instrumentation and has a higher sensitivity than IHA for the diagnosis of pulmonary hydatidosis.


  • 주제어

    Echinococcus granulosus .   pulmonary hydatidosis .   hydatid antigen .   dot immunobinding assay.  

  • 참고문헌 (5)

    1. Misterello G, Gentili M, Falagini P, Roncarolo D, Riva G, Tinelli M (1995) Dot immunobinding assay as a new diagnostic test for human hydatid disease. Immunol Lett 47: 79-85 
    2. Force L, Torres JM, Carillo A, Busca J (1992) Evaluation of eight serological tests in the diagnosis of human echinococcosis and follow-up. Clin Infect Dis 15: 473-480 
    3. Babba H, Messedi A, Masmoudi S (1994) Diagnosis of human hydatidosis: Comparison between imagery and six serologic techniques. Am J Trap Med Hyg 50: 64-68 
    4. King CH (2000) Echinococcosis. In Principles and Practice of Infectious Diseases, Mandell GL, Bennet JE, Dolin R (eds.). p2962-2963, Churchill Livingstone 5th ed. New York, USA 
    5. Devi CS, Parija SC (2003) A new serum hydatid antigen detection test for diagnosis of cystic echinococcosis. Am J Trap Med Hyg 69: 525-528 
  • 이 논문을 인용한 문헌 (1)

    1. 2011. "" Tuberculosis and respiratory diseases : TRD = 결핵 및 호흡기 질환, 70(4): 338~341     

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