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The Korean journal of parasitology v.43 no.1 = no.133, 2005년, pp.27 - 32   피인용횟수: 5

Genetic characteristics of the Korean isolate KI -1 of Toxoplasma gondii

LIN Aifen    (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center   ); SHIN Eun-Hee    (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center   ); KIM Tae-Yun    (Institute of Malariology and Department of Parasitology, Inje University College of Medicine   ); PARK Jae-Hwan    (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center   ); GUK Sang-Mee    (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center   ); CHAI Jong-Yil    (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Res  );
  • 초록

    Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5' and 3' ends of SAG2, respectively, with Mse I, Sau3A I, and Hha I, revealed that KI-1 is included in the genotype I, which includes the worldwide virulent RH strain. In addition, when the whole sequences of the coding regions of SAG1, rhoptry antigen 1 (ROP1), and GRA8 genes of KI-1 were compared with those of RH, minor nucleotide polymorphisms and amino acid substitutions were identified. These results show that KI-1 is a new geographical strain of T. gondii that can be included in the genotype I.


  • 주제어

    Toxoplasma gondii .   Korean isolate-1 (KI-1) .   PCR-RFLP .   sequence.  

  • 참고문헌 (14)

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    2. Johnson AM (1998) Is there more than one species in the genus Toxoplasma? Tokai J Exp Clin Med 23: 383-389 
    3. Howe DK, Sibley LD (1995) Toxoplasma gondii comprises three clonal lineages: Correlation of parasite genotype with human disease. J Infect Dis 172: 1561-1566 
    4. Sibley LD, Boothroyd JC (1992) Virulent strains of Toxoplasma gondii comprise a single clonal lineage. Nature 359: 82-85 
    5. Dubey JP, Graham DH, Blackston CR, Lehmann T, Gennari SM, Ragozo AM, Nishi SM, Shen SK, Kwok OC, Hill DE, Thulliez P (2002) Biological and genetic characterization of Toxoplasma gondii isolates from chickens (Gallus domesticus) from Sao Paulo, Brazil: unexpected findings. Int J Parasitol 32: 99-105 
    6. Lehmann T, Blackston CR, Parmley SF, Remington JS, Dubey JP (2000) Strain typing of Toxoplasma gondii: comparison of antigen-coding and housekeeping genes. J Parasitol 86: 960-971 
    7. Fazaeli A, Carter PE, Pennington TH (2000b) Intergenic spacer (IGS) polymorphism: a new genetic marker for differentiation of Toxoplasma gondii strains and Neospora caninum. J Parasitol 86: 716-723 
    8. Grigg ME, Ganatra J, Boothroyd JC, Margolis TP (2001b) Unusual abundance of atypical strains associated with human ocular toxoplasmosis. J Infect Dis 184: 633-639 
    9. Suzuki Y, Conley FK, Remington JS (1989) Differences in virulence and development of encephalitis during chronic infection vary with the strain of Toxoplasma gondii. J Infect Dis 159: 790-794 
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    11. Chai JY, Lin A, Shin EH, Oh MD, Han ET, Nan HW, Lee SH (2003) Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea. Korean J Parasitol 41: 147-154     
    12. Howe DK, Honore S, Derouin F, Sibley LD (1997) Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis. J Clin Microbiol 35: 1411-1414 
    13. Grigg ME, Bonnefoy S, Hehl AB, Suzuki Y, Boothroyd JC (2001a). Success and virulence in Toxoplasma as the result of sexual recombination between two distinct ancestries. Science 294: 161-165 
    14. Fazaeli A, Carter PE, Darde ML, Pennington TH (2000a) Molecular typing of Toxoplasma gondii strains by GRA6 gene sequence analysis. Int J Parasitol 30: 637-642 
  • 이 논문을 인용한 문헌 (5)

    1. 2008. "" The Korean journal of parasitology, 46(1): 45~48     
    2. 2008. "" The Korean journal of parasitology, 46(2): 105~108     
    3. 2010. "" The Korean journal of parasitology, 48(3): 195~201     
    4. 2010. "" The Korean journal of parasitology, 48(4): 325~329     
    5. 2011. "" The Korean journal of parasitology, 49(2): 115~123     

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  • 김태윤 (1)

  • Park, Jae-Hwan (12)

  • 국상미 (17)

  • Chai, Jong-Yil (149)

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