본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Evaluation of Different Culture Conditions of Clostridium bifermentans DPH-1 for Cost Effective PCE Degradation

Humayra Afroze Syeda    (United Graduate School of Agricultural Science, Gifu University   ); Hasegawa Yuki    (Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University   ); Nomura Izumi    (Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University   ); Chang Young C.    (United Graduate School of Agricultural Science, Gifu University   ); Sato Takeshi    (Department of Civil Engineering, Gifu University   ); Takamizawa Kazuhiro    (United Graduate School of Agricultural Science, Gifu University, Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University  );
  • 초록

    Clostridium bifermentans strain DPH-1 has already been found to dechlorinate perchloroethylene (PCE) to cis-dichloroethylene (cis-DCE) via trichloroethylene (TCE). In this study, our investigation on different culture conditions of this DPH-1 strain was extended to find a more efficient and cost effective growth medium composition for this DPH-1 strain in bioremediation practices. Temperature dependency of strain DPH-1 showed that the growth starting time and PCE degradation at $15^{\circ}C$ was very slow compared to that of $30^{\circ}C$ , but complete PCE degradation occurred in both cases. For the proper utilization of strain DPH-1 in more cost effective bioremediation practices, a simpler composition of an effective media was studied. One component of the culture medium, yeast extract, had been substituted by molasses, which served as a good source of electron donor. The DPH-1 strain in the medium containing molasses, in the presence of $K_{2}HPO_4\;and\;KH_{2}PO_4$ , showed identical bacterial multiplication (0.135 mg protein $mL^{-1}h^{-1}$ ) and PCE degradation rates ( $0.38\;{\mu}M/h$ ) to those of the yeast extract containing medium.


  • 주제어

    culture conditions .   PCE dechlorination .   Clostridium bifermentans DPH-1 .   molasses .   bioremediation.  

  • 참고문헌 (33)

    1. Infante, P. F. and T. A. Tsongas (1982) Mutagenic and oncogenic effects of chloromethanes, chloroethanes, and halogenated analogs of vinyl chloride. Environ. Sci. Res. 25: 301-327 
    2. Okeke, B. C., A. Paterson, J. E. Smith, and I. A. Watson- Craik (1997) Comparative biotransformation of pentachlorophenol in soils by solid substrate cultures of Lentinula edodes. Appl. Microbiol. Biotechnol. 48: 563-569 
    3. Neumann, A., H. Scholz-Muramatsu, and G. Diekert (1994) Tetrachloroethene metabolism of Dehalospirillum multivorans. Arch. Microbiol. 162: 295-301 
    4. Miller, E., G. Wohlfarth, and G. Diekert (1997) Comparative studies on tetrachloroethene reductive dechlorination mediated by Desulfitobacterium sp. Strain PCE-S. Arch. Microbiol. 168: 513-519 
    5. Schumacher, W. and C. Holliger (1996) The proton electron ratio of the menaquinone-dependent electron transport from dihydrogen to tetrachloroethene in Dehalobacter restrictus. J. Bacteriol. 178: 2328-2333 
    6. Magnuson, J. K., R. V. Stern, J. M. Gossett, S. H. Zinder, and D. R. Burris (1998) Reductive dechlorination of tetrachloroethene to ethene by a two component enzyme pathway. Appl. Environ. Microbiol. 64: 1270-1275 
    7. Miller, E., G. Wohlfarth, and G. Diekert (1998) Purification and characterization of the tetrachloroethene reductive dehalogenase of strain PCE-S. Arch. Microbiol. 169: 497-502 
    8. Suayama, A. M., S. Yamashita, S. Yoshino, and K. Furukawa (2002) Molecular characterization of the PceA reductive dehalogenase of Desulfitobacterium sp. strain Y51. J. Bacteriol. 184: 3419-3425 
    9. Okeke, B. C., Y. C. Chang, M. Hatsu, T. Suzuki, and K. Takamizawa (2001) Purification and cloning, and sequencing of an enzyme mediating the reductive dechlorination of tetrachloroethylene (PCE) from Clostridium bifermentans DPH-1. Can. J. Microbiol. 47: 448-456 
    10. Pietari, J. M. H. (1999) Development and Characterization of a Psychotropic Dechlorinating Culture and Temperature Response of a Mesophilic Dechlorinating Culture. M.S. Thesis. University of Washington, WA, USA 
    11. Distefano, T. D. (1999) The effect of tetrachloroethylene on biological dechlorination of vinyl chloride: Potential implication for natural bioattenuation. Water Res. 33: 1688-1694 
    12. Chang, Y. C., M. Hatsu, K. Jung, Y. S. Yoo, and K. Takamizawa (2000) Degradation of a variety of halogenated aliphatic compounds by an anaerobic mixed culture. J. Ferment. Bioeng. 86: 410-412 
    13. Holliger, C., G. Schraa, A. J. M., Stams, and A. J. B. Zehnder (1993) A highly purified enrichment culture couples the reductive dechlorination of tetrachloroethene to growth. Appl. Environ. Microbiol. 59: 2991-2997 
    14. Ni, S., J. K. Fredrickson, and L. Xun (1995) Purification and characterization of a novel 3-chlorobenzoate-reductive dehalogenase from the cytoplasmic membrane of Desulfomonile tiedjei DCB-1. J. Bacteriol. 177: 5135-5139 
    15. Fetzner, S. (1998) Bacterial dehalogenation. Appl. Micobiol. Biotechnol. 50: 633-657 
    16. Harkness, M. R. (2000) Economic considerations in enhanced aerobic biodegradation. pp. 9-14. In: G. B. Wickramananyake, A. R. Gavaskar, B. C. Alleman, and V. S. Magar (eds.). Bioremediation and Phytoremediation of Chlorinated and Recalcitrant Compounds. Battelle Press, Columbus, OH, USA 
    17. Silva, H. J., A. M. Giulietti, R. F. Segovia, and R. J. Ertola (1982) Use of molasses and whey in culture media for the development and production of a toxin from Clostridium perfringens type D. Rev. Argent. Microbiol. 14: 85-90 
    18. Vanneli, T., M. Logan, D. M. Arciero, and A. B. Hooper (1990) Degradation of halogenated aliphatic compounds by the ammonia-oxidizing bacterium Nitrosomonas europaea. Appl. Environ. Microbiol. 60: 542-548 
    19. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254 
    20. U.S. Environmental Protection Agency (1985) Substances Found at Proposed and Final NPL Sites Through Update Number Three. Document NPL-U3-6-3. US Environmental Protection Agency, Washington, D.C., USA 
    21. Maymo-Gatell, X., Y. Chien, J. M. Gossett, and S. H. Zinder (1997) Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene. Science 276: 1568-1571 
    22. Bradley, P. M., F. H. Chapelle, and D. R. Lovley (1998) Humic acids as electron acceptors for anaerobic microbial oxidation of vinyl chloride and dichloroethene. Appl. Environ. Microbiol. 64: 3102-3105 
    23. Neumann, A., G. Wohlfarth, and G. Diekert (1995) Properties of tetrachloroethene dehalogenase of Dehalospirillum multivorans. Arch. Microbiol. 163: 276-281 
    24. de Bruin, W. P., M. J. J. Kotterman, M. A., Posthumus, G., Schraa, and A. J. B. Zehnder (1992) Complete biological reductive transformation of tetrachloroethene to ethane. Appl. Environ. Microbiol. 58: 1996-2000 
    25. Ensley, B. D. (1991) Biochemical diversity of trichloroethylene metabolism. Annu. Rev. Microbiol. 45: 283-299 
    26. Malachowsky, K. J., T. J. Phelps, A. B. Teboli, D. E. Minnikin, and D. C. White (1994) Aerobic mineralization of trichloroethylene, vinyl chloride and aromatic compounds by Rhodococcus species. Appl. Environ. Microbiol. 60: 542-548 
    27. Gossett, J. M. (1987) Measurement of Henry's law constants for C1 and C2 chlorinated hydrocarbons. Environ. Sci. Technol. 21: 202-208 
    28. Ellis, D. E., E. J. Lutz, R .J. Odom, Jr. Buchanan, M. D. Lee, C. L. Bartlett, M. R. Harkness, and K. A. Deweered (2000) Bioaugmentation for accelerated in situ anaerobic bioremediation. Environ. Sci. Technol. 34: 2254-2260 
    29. Maymo-Gatell, X., V. Tandoi, J. M. Gossett, and S. H. Zinder (1995) Characterization of an $H_2$-utilizing enrichment culture that reductively dechlorinates tetrachloroethene to vinyl chloride and ethane in the absence of methanogenesis and acetogenesis. Appl. Environ. Microbiol. 61: 3928-3933 
    30. Chang, Y. C., M. Hatsu, K. Jung, Y. S. Yoo, and K. Takamizawa (2000) Isolation and characterization of a tetrachloroethylene dechlorinating bacterium, Clostridium bifermentans DPH-1. J. Biosci. Bioeng. 89: 489-491 
    31. Harkness, M. R., A. A. Bracco, M. J. Jr. Brennan, K. A. DeWeerd, and J. L. Spivack, (1999) Use of Bioaugmentation to stimulate complete reductive dechlorination of trichloroethene in Dover soil columns. Environ. Sci. Technol. 33: 1100-1109 
    32. Fathepure, B. Z., J. P. Nengu, and S. A. Boyd (1987) Anaerobic bacteria that dechlorinate perchloroethylene. Appl. Environ Microbiol. 53: 2671-2674 
    33. Sung, Y., K. M. Ritalahti, R. A. Sanford, J. W. Urbance, S. J. Flynn, J. M. Tiedje, and F. E. Loffler (2003) Characterization of two tetrachloroethene-reducing, acetate-oxidizing anaerobic bacteria and their description as Desulfuromonas michiganensis sp. Appl. Environ. Microbiol. 69: 2964- 2974 

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기