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Biotechnology and bioprocess engineering v.10 no.1, 2005년, pp.60 - 66   피인용횟수: 13

Cytotoxic, Antioxidative, and ACE Inhibiting Activities of Dolsan Leaf Mustard Juice (DLMJ) Treated with Lactic Acid Bacteria

Yoo Eun-Jeong    (Department of Biotechnology, Yosu National University   ); Lim Hyun-Soo    (Department of Biotechnology, Yosu National University   ); Park Kyung-Ok    (Department of Biotechnology, Yosu National University   ); Choi Myeong-Rak    (Department of Biotechnology, Yosu National University  );
  • 초록

    This study was performed to know whether there is any change of physiological activity in DLMJ which is inoculated by lactic acid bacteria. Lactic acid bacteria were isolated from Dolsan leaf mustard Kimchi (DLMK) at $20^{\circ}C$ . In the optimum ripening period, the population of Leuconostoc and Lactobacilli in the DLMK were found to be high. The Leuconostoc, Lactobacilli and Lactococci strains were identified as Leuconostoc mesenteroides, Leuconostoc gelidum, Weissella confusa, Lactobacillus plantarum, Lactobacillus raffinolactis, Lactococcus lactis and Weissella confusa using the Biolog system. The most predominant strain which was isolated from DLMK was Weissella confusa. As the results of the phylogenetic analysis using 16s rDNA sequence, the Weissella confusa turned out to be Weissella kimchii, with 99.0% similarity. To investigated the change of physiological activity in DLMJ by lactic acid bacteria, 7 predominant strains inoculated to DLMJ (Dolsan Leaf Mustard Juice). The cytotoxicity was found to be under $19.55\%$ all cases. Also, the antioxidative activity of the DLMJ treated with lactic acid bacteria was very low, which might have been due to the reduced antioxidative phytochemicals during the preparation of the sterile sample. The ACE inhibiting activity of DLMJ by inoculation with Weissella kimchii was shown to be the highest ( $94.0\%$ ). This could be that the degradation of sulfur containing materials in DLMJ by Weissella kimchii gave rise to ACE inhibiting activity.


  • 주제어

    dolsan leaf mustard juice .   Weissella kimchii .   cytotoxicity .   antioxidative .   ACE inhibiting activity.  

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    7. Hyun, Mee-Sun ; Hur, Jung-Mu 2011. "Lactic Acid Fermentation of Dioscorea batatas and Its Anti-Inflammatory Effects on TNBS-induced Colits Model" Journal of applied biological chemistry, 54(1): 51~55     
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    11. Bae, Sang-Ok ; Wi, Sung-Dol ; Lim, Hyun-Soo ; Choi, Myeong-Rak 2011. "Change in the Physicochemical Properties of Dolsan Leaf Mustard under Salting Conditions" 한국식품저장유통학회지 = Korean journal of food preservation, 18(4): 502~507     
    12. Oh, SunKyung ; Kim, KiWoong ; Bae, SangOk ; Choi, Myeong Rak 2015. "Sinigrin content of different parts of Dolsan leaf mustard" 한국식품저장유통학회지 = Korean journal of food preservation, 22(4): 553~558     
    13. Oh, Sun Kyung ; Tsukamoto, Chigen ; Kim, Ki Woong ; Choi, Myeong Rak 2016. "LC-PDA/MS/MS Analysis of Glucosinolates in Dolsan Leaf Mustard Kimchi and Dolsan Leaf Mustard Pickles" KSBB Journal, 31(1): 1~7     

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