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Biotechnology and bioprocess engineering v.10 no.1, 2005년, pp.73 - 77   피인용횟수: 2

Enhancement of Growth and Secondary Metabolite Biosynthesis: Effect of Elicitors Derived from Plants and Insects

Jeong Gwi-Taek    (Engineering Research Institute, Chonnam National University   ); Park Don-Hee    (School of Biological Sciences and Technology, Chonnam National University, Faculty of Applied Chemical Engineering, Chonnam National University, Biotechnology Research Institute, Chonnam National University  );
  • 초록

    Plant-derived natural products have been and will continue to be valuable sources. Elicitors have been employed to modify cell metabolism in order to enhance the productivity of useful metabolites in plant cell/tissue cultures. In this study, several elicitors were used to improve the productivity of useful metabolites and to reduce culture time for archiving high concentration in P. ginseng hairy root cultures. The addition of chitosan, chitosan oligosaccharide and alginate oligosaccharide to the culture of P. ginseng hairy roots caused growth to be inhibited with the increase in elicitor concentration. The usage of the chitosan elicitor and D-glucosamine caused a slight decrease in hairy root growth, whereas total ginseng saponin accumulated slightly with the increase in elicitor concentration. When gel beads were added to the culture medium at the initial period, hairy root growth was enhanced. The maximum growth was 1.35 times higher than that of the control at $1\%$ (w/v). Total ginseng saponin content decreased due to the addition of alginate beads. This would result in consistent diffusion of lower levels of calcium ions during the culture period that promotes biomass growth.


  • 주제어

    Panax ginseng .   hairy root .   elicitor .   chitosan .   alginate bead .   secondary metabolite.  

  • 참고문헌 (12)

    1. Ramachandra R. S. and G. A. Ravishankar (2002) Plant cell cultures: Chemical factories of secondary metabolites. Biotechnol. Adv. 20: 101-153 
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    3. Akimoto, C., H. Aoyagi, and H. Tanaka (1999) Endogenous elicitor-like effects of alginate on physiological activities of plant cells. Appl. Microbiol. Biotechnol. 52: 429-436 
    4. Jeong, G. T., D. H. Park, B. Hwang, and J. C. Woo (2003) Comparison of growth characteristics of Panax ginseng hairy roots in various bioreactors. Appl. Biochem. Biotechnol. 105-108: 493-503 
    5. Natsume, M., Y. Kamo, M. Hirayama, and T. Adachi (1994) Isolation and characterization of alginate-derived oligosaccharides with root growth-promoting activities. Carbohydr. Res. 258: 187-197 
    6. Akimoto, C., H. Aoyagi, and H. Tanaka (1999) Endogenous elicitor-like effects of alginate on physiological activities of plant cells. Appl. Microbiol. Biotechnol. 52: 429-436 
    7. Banthorpe, D. V. (1994) Secondary metabolism in plant tissue culture: Scope and limitations. Nat. Prod. Rep. 303-328 
    8. Lee, J. H., N. H. Loc, T. H. Kwon, and M. S. Yang (2004) Partitioning of recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) from plant cell suspension culture in PEG/sodium phosphate aqueous two-phase systems. Biotechnol. Bioprocess Eng. 9: 12-16 
    9. Lee, S. Y., J. M. Cho, and D. I. Kim (2003) Stability enhancement of hGM-CSF in transgenic Nicotiana tabacum suspension cell cultures. Biotechnol. Bioprocess Eng. 8: 187-191 
    10. Uozumi, N. and T. Kobayashi (1994) Application of hairy root and bioreactors. pp. 307-338. In: D. D. Y. Ryu and S. Furusaki (eds.). Advances in Plant Biotechnology. Elsevier, NY, USA 
    11. Kim, J. H., J. H. Shin, H. J. Lee, I. S. Chung, and H. J. Lee (1997) Effect of chitosan on indirubin production from suspension culture of Polygonum tinctorium. J. Fermentation Bioeng. 83: 206-208 
    12. Sudha, G. and G. A. Ravishankar (2002) Involvement and interaction of various signaling compounds on the plant metabolic events during defense response, resistance to stress factors, formation of secondary metabolites and their molecular aspects. Plant Cell Tissue Organ Culture 71: 181-212 
  • 이 논문을 인용한 문헌 (2)

    1. 2007. "" Biotechnology and bioprocess engineering, 12(5): 463~469     
    2. Kim, Yu-Jin ; Sim, Ju-Sun ; ; Lee, Chung-Hyae ; In, Jun-Gyo ; Lee, Bum-Soo ; Yang, Deok-Chun 2008. "The Effect of NaCI on the Growth and Ginsenoside Production from Ginseng Hairy Root" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 16(2): 94~99     

 저자의 다른 논문

  • Jeong Gwi-Taek (1)

    1. 2006 "Characteristics of Transformed Panax ginseng C.A. Meyer Hairy Roots: Growth and Nutrient Profile" Biotechnology and bioprocess engineering 11 (1): 43~47    
  • Park Don-Hee (2)

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