본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Biotechnology and bioprocess engineering v.10 no.1, 2005년, pp.91 - 98   피인용횟수: 2

Metabolic Flux Analysis of Beijerinckia indica for PS-7 Production

Wu Jian-Rong    (Department of Biotechnology and Bioengineering, Pukyong National University   ); Son Jeong Hwa    (Department of Biotechnology and Bioengineering, Pukyong National University   ); Seo Hyo-Jin    (Interdisciplinary Program of Marine Biotechnology, Pukyong National University   ); Kim Ki-Hong    (Aquatic Life of Medicine, Pukyong National University   ); Nam Yoon-Kwon    (Aquaculture, Pukyong National University   ); Lee Jin-Woo    (Division of Biotechnology, Dong-A University   ); Kim Sung-Koo    (Department of Biotechnology and Bioengineering, Pukyong National University  );
  • 초록

    In order to investigate central metabolic changes in Beijerinckia indica, cells were grown on different carbon sources and intracellular flux distributions were studied under varying concentrations of nitrogen. Metabolic fluxes were estimated by combining material balances with extracellular substrate uptake rate, biomass formation rate, and exopolysaccharide (EPS) accumulation rate. Thirty-one metabolic reactions and 30 intracellular metabolites were considered for the flux analysis. The results revealed that most of the carbon source was directed into the Entner-Doudoroff pathway, followed by the recycling of triose-3-phosphate back to Hexose­6-phosphate. The pentose phosphate pathway was operated at a minimal level to supply the precursors for biomass formation. The different metabolic behaviors under varying nitrogen concentrations were observed with flux analysis.

  • 주제어

    metabolic flux analysis .   flux distribution .   Beijerinckia indica .   PS-7 .   exopolysaccharide.  

  • 참고문헌 (25)

    1. Gulin, S., A. Kussak, P. E. Jansson, and G. Widmalm (2001) Structural studies of S-7, another exocellular polysaccharide containing 2-deoxy-arabino-hexuronic acid. Carbohyd. Res. 311: 285-290 
    2. Cha, W. S., D. B. Choi, and S. H. Kang (2004) Optimization of culture meidia for solid-state culture of pleurotus feruae. Biotechnol. Bioprocess Eng. 9: 369-373     
    3. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith (1956) Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356 
    4. Klinke, S., M. Dauner, G. Scott, B. Kessler, and B. Witholt (2000) Inactivation of isocitrate lyase leads to increased production of medium chain length poly (3-hydroxyalkanoates). Appl. Environ. Microbiol. 66: 929-913 
    5. Ampe, F., J. L. Uribelarrea, G. M. F. Aragao, and N. D. Lindley (1997) Benzoate degradation via the ortho pathway in Alcaligenes eutrophus is perturbed by succinate. Appl. Environ. Microbiol. 63: 2765-2770 
    6. ttp://www.genome.jp/kegg/pathway/ 
    7. Kang, K. S. and W. H. McNeely (1976) Polysaccharide and bacterial fermentation process for its preparation. US Patent 3,960,832 
    8. http://pseudocyc.pseudomonas.com:1555/ 
    9. Portais, J. C., P. Tavernier, I. Gosselin, and J. N. Barbotin (1999) Cyclic organization of the carbohydrate metabolism in Sinohizobium meliloti. Eur. J. Biochem. 265: 473-480 
    10. Valino, J. J. and G. Stephanopoulos (1993) Metabolic flux distribution in Corynebacterium glutmincum during growth and lysine overproduction. Biotechnol. Bioeng. 41: 633- 646 
    11. Thorne, L., M. J. Mikolajczak, R. W. Armentrout, and T. J. Pollock (2000) Increasing the yield and viscosity of exopolysaccharide secreted by Sphingomonas by augmentation of chromosomal genes with multiple copies of cloned biosynthetic genes. J. Ind. Microbiol. Biotechnol. 25: 49-57 
    12. Lee, S. Y. and E. T. Papoutsakis (1999) Metabolic Engineering. Marcel Dekker, NY, USA 
    13. Koroleff, F. (1970) Direct Determination of Ammonia in Natural Waters as Indophenol Blue: In Information on Techniques and Methods for Seawater Analysis. pp. 19-22. Internat. Counc. Exploration of the sea, Charlottenlund, Norway 
    14. Ketusse, F., P. Chevallereau, J. L. Simon, and N. Lindley (2002) The influence of metabolic network structures and energy requirements on xanthan gum yields. J. Biotechnol. 99: 307-317 
    15. Neidhardt, F. C., J. L. Ingraham, and M. Schaechter (1990) Physiology of the Bacterial Cell: A Molecular Approach. Sinauer Associates Inc., Sunderland, Mass, UK 
    16. http://mbel.kaist.ac.kr/ 
    17. Beale, J. M. Jr. and J. L. Foster (1996) Carbohydrate fluxes into alginate biosynthesis in Azotobacter vinelandii NCIB 8789: NMR investigations of the triose pools. Biochemistry 35: 4492-4501 
    18. http://genome.ornl.gov/microbial/avin/ 
    19. Lee, J. W., W. G. Yeomans, A. L. Allen, R. A. Gross, and D. L. Kaplan (1997) Compositional consistency of a heteropolysaccharide- 7 produced by Beijerinckia indica. Biotechnol. Lett. 19: 803-807 
    20. Vartak, B. N., C. C. Lin, J. M. Cleary, M. J. Fagan, and M. H. Jr. Saier (1995) Glucose metabolism in Sphingmonas elodea: pathway engineering via construction of a gucose- 6-phosphate dehydrogenase insertion mutant. Microbiology 141: 2339-2350 
    21. Cataldo, D. A., M. Haroon, I. E. Scharader, and V. L. Youngs (1975) Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Commu. Soil Sci. Plan. 6: 71-80 
    22. Schweizer, H. P. and P. Cecilia (1996) Regulation of glycerol metabolism in Pseudomonas aeruginosa: Characterization of the glpR repressor gene. J. Bacteriol. 178: 5215-5221 
    23. Gosselin, I., O. Wattraint, D. Riboul, J. N. Barbotin, and J. Charles (2001) A deep investigation on carbohydrate cycling in Sinohizobium meliloti. FEBS Lett. 499: 45-49 
    24. Standford, P. A. and J. Baird (1983) Industrial Utilization of Polysaccharide: In Polysaccharide II. Academic Press, London, UK 
    25. Choi, A. S. G., Kim, B. D. Yoon, and H. M. Oh (2003) Growth and amino acid contents of spirulina platensis with different nitrogen sources. Biotechnol. Bioprocess Eng. 8: 368-374     
  • 이 논문을 인용한 문헌 (2)

    1. Wu Jian-Rong ; Son Jeong Hwa ; Kim Ki Myong ; Nam Soo-Wan ; Lee Jin-Woo ; Kim Sung-Koo 2005. "Optimization of Heteropolysaccharide-7 Production by Beijerinckia Indica" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 33(2): 117~122     
    2. Jin, Il-Hyuck ; Jung, Dae-Young ; Son, Chang-Woo ; Kim, Sung-Koo ; Gao, Wa ; Chung, Chung-Han ; Lee, Jin-Woo 2011. "Enhanced Production of Heteropolysaccharide-7 by Beijerinckia indica HS-2001 in Repeated Batch Culture with Optimized Substitution of Culture Medium" Biotechnology and bioprocess engineering, 16(2): 245~255     

 저자의 다른 논문

  • 손정화 (7)

    1. 1998 "생물고분자 막 형성을 이용한 동물세포 배양 및 단클론항체 생산" 한국생물공학회지 = Korean journal of biotechnology and bioengineering 13 (1): 13~19    
    2. 2003 "Effect of Lactobacillus fermentum MG590 on Alcohol Metabolism and Liver Function in Rats" Journal of microbiology and biotechnology 13 (6): 919~925    
    3. 2004 "Mass Production of HzSNPV Baculoviruses in Immobilized Heliothis zea (HzAM1) Insect Cell Culture" Biotechnology and bioprocess engineering 9 (5): 352~355    
    4. 2004 "Characterization of Bacteriocin Produced by Lactobacillus bulgaricus" Journal of microbiology and biotechnology 14 (3): 503~508    
    5. 2005 "Beijerinckia Indica 배양을 통한 Heteropolysaccharide-7 생산 최적화" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology 33 (2): 117~122    
    6. 2005 "Development of DNA Vaccine Against Red Sea Bream Iridovirus (RSIV)" Journal of microbiology and biotechnology 15 (4): 873~879    
    7. 2005 "Optimization of Chitosan-Alginate Encapsulation Process Using Pig Hepatocytes or Development of Bioartificial Liver" Journal of microbiology and biotechnology 15 (1): 7~13    
  • Seo, Hyo-Jin (17)

  • Kim, Ki-Hong (67)

  • Nam, Yoon-Kwon (73)

  • 이진우 (44)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역


  • NDSL :

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기