본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of plant biology = 식물학회지 v.48 no.1, 2005년, pp.85 - 95   피인용횟수: 3

Low Expression Profiles of Heat Stress-Related Genes in Capsicum annuum

Ashrafuzzaman M.    (Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University   ); Oh S.June    (Department of Pharmacology, Pharmacogenomics Research Center, College of Medicine, Inje University   ); Hong Choo Bong    (Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University  );
  • 초록

    A cDNA library was constructed for hot pepper plants that had been heat-shock-treated. We used a modified differential screening method, double negative screening, to isolate 500 cDNA clones that represented genes with low expression levels under conditions of high-temperature stress. Of those 500 clones, 200 were randomly selected for single-read sequencing from the 5' ends. After annotation with Blastx, the sequence was applied to InterProScan to scan for functional motifs of proteins. Among the cDNA clones analyzed, about $41\%$ of the ESTs could not be functionally classified. However, of those that could be, the largest portion of the ESTs $(15\%)$ were assigned to the category of cell rescue and defense; genes involved in cell cycle/DNA processing constituted the smallest group, comprising $1\%$ of the ESTs. Genes related to energy and protein fates constituted the second $(10\%)$ and third $(9\%)$ largest groups, respectively. Finally, $3\%$ of the ESTs were assigned to transcription, and $2\%$ to signal transduction. The high portion of unclassified ESTs probably resulted from the screening method, which was designed for low-expression messages. Likewise, the high number of ESTs for cell rescue and defense suggests that many genes with low levels of expression are associated with the stress response.


  • 참고문헌 (43)

    1. Basha E, Lee GJ, Breci LA, Hausrath AC, Buan NR, Giese KC, Vierling E (2004) The identity of proteins associated with a small heat shock potein during heat stress in vivo indicates that these chaperones protect a wide range of cellular functions. J Biol Chem 279: 7566-7575 
    2. Chen W, Provart NJ, Glazebrook J, Katagiri F, Chang HS, Eulgem T, Mauch F, Luan SH, Zou G, Whitham SA, Budworth PR, Tao Y, Xie Z, Chen X, Lam S, Kreps JA, Harper JF, Si-Ammour A, Mauch-Mani B, Heinlein M, Kobayashi K, Hohn T, Dangl JL, Wang X, Zhu T (2002) Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses. Plant Cell 14: 559-574 
    3. Gubler F, Chandler PM, White RG, Llewellyn DJ, Jacobsen JV (2002) Gibberellin signaling in barley aleurone cells. Control of SLN1 and GAMYB expression. Plant Physiol 129: 191-200 
    4. Kawasaki S, Borchert C, Oeyholos M, Wang H, Brazille S, Kawai K, Galbraith D, Bohnert HJ (2001) Gene expression profiles during the initial phase of salt stress in rice. Plant Cell 13: 889-905 
    5. Kore-eda S, Cushman MA, Akselrod I, Bufford D, Fredrickson M, Clark E, Cushman JC (2004) Transcript profiling of salinity stress responses by large-scale expressed sequence tag analysis in Mesembryanthemum crystallinum. Gene 341: 83-92 
    6. Liu Y, Schiff M, Serino G, Deng XW, Oinesh-Kumar SP (2002) Role of SCF ubiquitin-ligase and the COP9 signalosome in the N gene-mediated resistance response to tobacco mosaic virus. Plant Cell 14: 1483-1496 
    7. Salmeron JM, Oldroyd GED, Rommens CMT, Scofield SR, Kim H-S, Lavelle DT, Dahlbeck D, Staskawicz BJ (1996) Tomato Prf is a member of the leucine-richrepeat class of plant disease resistance genes and lies embedded within the Pto kinase gene cluster. Cell 86: 123-133 
    8. Schaller A (2004) A cut above the rest: The regulatory function of plant proteases. Planta 220: 183-197 
    9. Sun W, van Montagu M, Verbruggen N (2002) Small heat shock proteins and stress tolerance in plants. Biochim Biophys Acta 1577: 1-9 
    10. van der Hoeven RY, Ronning C, Giovannoni JJ, Martin G, Tanksley SD (2002) Deductions about the number, organization and evolution of genes in the tomato genome based analysis of a large EST collection and selective genomic sequencing. Plant Cell 14: 1441-1456 
    11. Wei Y, Waltz DA, Rao N, Drummond RJ, Rosenberg S, Chapman HA (1994) Identification of the urokinase receptor as an adhesion receptor for vitronectin. J Biol Chem 269: 32380-32388 
    12. Azevedo C, Sadanandom A, Kitagawa K, Freialdenhoven A, Shirasu K, Schulze-Lefert P (2002) The RAR1 interactor SGT1, an essential component of R gene-triggered disease resistance. Science 295: 2073-2076 
    13. Bonaldo MF, Lennon G, Soares MB (1996) Normalization and subtraction: Two approaches to facilitate gene discovery. Genome Res 6: 791-806 
    14. Carninci P, Shibata Y, Hayatsu N, Sugahara Y, Shibata K, Itoh M, Konno H, Okazaki Y, Muramatsu M, Hayashizaki Y (2000) Normalization and subtraction of captrapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes. Genome Res 10: 1617-1630 
    15. Cho VB, Jean HJ, Hong CB (2003) Cloning and functional annotation of rare mRNA species from drought-stressed hot pepper (Capsicum annuum). J Plant Biol 46: 83-89     
    16. Falk S, Maxwell DP, Laudenbach DE, Huner NPA (1996) Photosynthetic adjustment to temperature. In NR Baker, ed, Photosynthesis and the Environment. Kluwer Academic, Dordrecht, pp 367-385 
    17. Margolin JF, Friedman JR, Meyer WK, Vissing H, Thiesen HJ, Rauscher FJR (1994) Kruppel-associated boxes are potent transcriptional activation system for regulated gene expression in transgenic plants. Proc Natl Acad Sci USA 91: 4509-4513 
    18. Asamizu E, Nakamura Y, Sato S, Tabata S (2000) A large scale analysis of cDNA in Arabidopsis thaliana: Generation of 12,028 non-redundant expressed sequence tags from normalized and size-selected cDNA libraries. DNA Res 7: 175-180 
    19. Seki M, Narusaka M, Abe H, Kasuga M, Yamaguchi-Shinozaki K, Carninci P, Hayashizaki Y, Shinozaki K (2001) Monitoring the expression pattern of 1,300 Arabidopsis genes under drought and cold stresses by using a fulllength cDNA microarray. Plant Cell 13: 61-72 
    20. Bohnert HJ, Ayoubi P, Borchert C, Bressan RA, Burnap RL, Cushman JC, Cushman MA, Deyholos M, Fischer R, Galbraith DW (2001) A genomics approach towards salt stress tolerance. Plant Physiol Biochem 39: 295-311 
    21. Borovskii GB, Stupnikova IV, Antipina AI, Vladimirova SV, Voinikov VK (2002) Accumulation of dehydrin-like proteins in the mitochondria of cereals in response to cold, freezing, drought and ABA treatment. BMC Plant Biol 2:5-12 
    22. Fernandez P, Paniego N, Lew S, Hopp HE, Heinz RA (2003) Differential representation of sunflower ESTs in enriched organ-specific cDNA libraries in a small scale sequencing project. BMC Genomics 4: 40-49 
    23. Jin H, Martin C (1999) Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol 41: 577-585 
    24. Sambrook L, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, Ed 2. Cold Spring Harbor Laboratory Press, New York 
    25. Park SM, Hong CB (2001) Class I small heat-shock protein gives thermotolerance in tobacco. J Plant Physiol 159: 25-30 
    26. Rabbani MA, Maruyama K, Abe H, Khan MA, Katsura K, Ito Y, Yoshiwara K, Seki M, Shinozaki K, YamaguchiShinozaki K (2003) Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses. Plant Physiol 133: 1755-1767 
    27. Clark MD, Hennig S, Herwig R, Clifton SW, Marra MA, Lehrach H, Johnson SL, Group tW, WU-GSCnEST Group (2001) An oligonucleotide fingerprint normalized and expressed sequence tag characterized zebrafish cDNA library. Genome Res 11: 1594-1602 
    28. Cook T, Gebelein B, Belal M, Mesa K, Urrutia R (1999) Three conserved transcriptional repressor domains are a defining feature of the TIEG subfamily of Sp1-like zinc finger proteins. J Biol Chem 274: 29500-29504 
    29. Seki M, Narusaka M, Ishida L Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Taji T, Yamaguchi-Shinozaki K, Carninci P, Kawai L Hayashizaki Y, Shinazaki K (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a fulllength cDNA microarray. Plant J 31: 279-292 
    30. Wang W, Vinocur B, Altman A (2003) Plant responses to drought, salinity and extreme temperatures: Towards genetic engineering for stress tolerance. Planta 218: 114 
    31. Wang W, Vinocur B, Shoseyov OI, Altman A (2004) Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci 9: 244-253 
    32. Fowler S, Thomashow MF (2002) Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell 14: 1675-1690 
    33. Carson DL, Botha FC (2000) Preliminary analysis of expressed sequence tags for sugarcane. Crop Sci 40: 1769-1779 
    34. Kreps JA, Wu Y, Chang HS, Zhu T, Wang X, Harper JF (2002) Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol 130: 2129-2141 
    35. Tezara W, Mitchell VJ, Driscoll SD, Lawlor DW (1999) Water stress inhibits plant photosynthesis by decreasing coupling factor and ATP. Nature 401: 914-917 
    36. Reddy AR, Ramakrishna AC, Sekhar AC, Nagablushana I, Ravindra Babu P, Bonaldo MF, Soares MB, Bennetzen JI, (2002) Novel genes are enriched in normalized cDNA libraries from drought-stressed seedlings of rice (Oryza sativa L. subsp. indica cv. Nagina 22). Genome 45: 204-211 
    37. Ohlrogge J, Benning C (2000) Unraveling plant metabolism by EST analysis. Curr Opin Plant Biol 3: 224-228 
    38. del Pozo JC, Estelle M (1999) Function of the ubiquitinproteasome pathway in auxin response. Trends Plant Sci 4: 107-112 
    39. Pellegrineschi A, Reynolds M, Pacheco M, Brito RM, Almeraya R, Yamaguchi-Peng Z, Staub JM, Serino G, Kwok SF, Kurepa J, Bruce BD, Vierstra RD, Wei N, Deng XW (2001) The cellular level of PR500, a protein complex related to the 19S regulatory particle of the proteasome, is regulated in response to stresses in plants. Mol Biol Cell 12: 383-392 
    40. Shinazaki K, Yamaguchi-Shinozaki K (1999) Molecular responses to drought stress. In K Shinozaki, K Yamaguchi-Shinozaki, eds, Molecular Responses to Cold, Drought, Heat and Salt Stress in Higher Plants. RG Landes, Austin, pp 11-28 
    41. Altschul SF, Madden TL, Schaffer M, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucl Acids Res 25: 3389-3402 
    42. Chinnusamy V, Schumaker K, Zhu JK (2004) Molecular genetic perspectives on cross-talk and specificity in abiotic stress signaling in plants. J Exp Bot 55: 225-236 
    43. Huckelhoven R (2004) Bax inhibitor-1, an ancient cell death suppressor in animals and plants with prokaryotic relatives. Apoptosis 9: 299-307 
  • 이 논문을 인용한 문헌 (3)

    1. 2006. "" Journal of plant biology = 식물학회지, 49(6): 484~490     
    2. 2006. "" Journal of plant biology = 식물학회지, 49(4): 298~302     
    3. 2007. "" Journal of plant biology = 식물학회지, 50(4): 496~503     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기