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Genome Constitution and Classification Using Retrotransposon­Based Markers in the Orphan Crop Banana

Teo Chee How    (Department of Biotechnology, Faculty of Food Science and Biotechnology, Universiti Putra Malaysia, Department of Biology, University of Leicester   ); Tan Siang Hee    (Molecular Biology Department, Sime Darby Technology, Centru Sdn Bhd   ); Ho Chai Ling    (Department of Biotechnology, Faculty of Food Science and Biotechnology, Universiti Putra Malaysia   ); Faridah Qamaruz Zaman    (Department of Biology, Faculty of Science and Environmental Studies, Universiti Putra Malaysia   ); Othman Yasmin Rofina    (Institute of Biological Sciences, Universiti Malaya   ); Heslop-Harrison John Seymour    (Department of Biology, University of Leicester   ); Kalendar Ruslan    (MTT/BI Plant Genomics Laboratory, Institute of Biotechnology, University of Helsinki   ); Schulman Alan Howard    (MTT/BI Plant Genomics Laboratory, Institute of Biotechnology, University of Helsinki, Plant Breeding Biotechnology, MTT Agrifood Research Finland  );
  • 초록

    We have exploited the repetitive and dispersed nature of many long terminal repeat (LTR)-retrotransposon families for characterizing genome constitutions and classifying cultivars of the genus Musa. Insertional polymorph isms of the elements were studied using seven published and two newly designed primers facing outwards from the LTRs and reverse transcriptase (RT) domain of the retrotransposon. The primers generated specific amplification patterns showing the universal applicability of this marker type. The Inter-Retrotransposon Amplified Polymorphism (IRAP) markers distinguished the A and B genomes of the banana species (Musa acuminata Colla and Musa balbisiana Colla) and between banana cultivars. The IRAP markers enabled phylogenetic analysis of 16 Malaysian banana cultivars and determination of the genome constitution of hybrid banana (AAB, ABB, AABB, and AAAB), and gave information about ancestral genotypes of the hybrids. In addition, the IRAP detected new retrotransposon insertions into the genome of tissue culture regenerants. This PCR-based IRAP assay is amenable to large-scale throughput demands in screening breeding populations and is applicable for any crop.

  • 참고문헌 (28)

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