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Journal of plant biology = 식물학회지 v.48 no.1, 2005년, pp.142 - 146   피인용횟수: 3

Effect of Plant Growth Regulators on Regeneration of Plantlets from Bud Cultures of Cymbopogon nardus L. and the Detection of Essential Oils from the in Vitro Plantlets

Chan Lai Keng    (School of Biological Sciences, Universiti Sains Malaysia   ); Dewi Patra Rina    (School of Biological Sciences, Universiti Sains Malaysia   ); Boey P.L.    (School of Chemical Sciences, Universiti Sains Malaysia  );
  • 초록

    Cymbopogon nardus L. could be propagated via tissue culture using axillary buds as explants. The aseptic bud explants obtained using double sterilization methods produced stunted abnormal multiple shoots when they were cultured on Murashige and Skoog medium (MS) supplemented with 1.0mg $L^{-1}$ or 2.0mg $L^{-1}$ benzyladenine (BA). Stunted shoots that cultured on MS + 1.0 mg $L^{-1}$ BA+1.0 mg $L^{-1}$ $N^6-isopentenyl-adenine$ (2iP) could induce elongation of shoots from about $60\%$ of the stunted shoots. Normal multiple shoots could be induced at the highest (19.7 shoots per bud) from the bud explahts within six weeks when cultured on proliferation medium consisted of MS supplemented with 0.3 mg $L^{-1}$ BA and 0.1 mg $L^{-1}$ indole-3-butyric acid (IBA). The separated individual shoot produced roots when transferred to basic MS solid medium. The essential oils that were contained in the mature plants namely citronellal, geraniol and citronellol were also found in the in vitro C. nardus plant-lets. Citronellal was the main essential oil component in the matured plants while geraniol was the main component in the in vitro plantlets.


  • 참고문헌 (10)

    1. Chan LK, Patra RD (2000) In vitro culture of Cymbapagan citrstus and C. nardus: problems and prospects, In YS Chang, V Subramaniam, AS Zainin, S Khozirah, eds, Medicinal plants: Quality herbal products for healthy living. Proceeding of the conference on forestry and forest products research (CFFPR) series, Kepong, Kuala Lumpur, pp 154-158 
    2. Ghani AKA, Rahman AA, Alimon H, Din LB (1991) Kultur tisu dan penghasilan minyak pati daripada Cymbapagon nardus Linn. Rendle. In KH Neumann, W Barz, E Reinhard, eds, Medicinal products from tropical rain forest. Proceedings of the conference. Kuala Lumpur, pp 69-78 
    3. Henderson MR (1954) Malayan Wild Flowers, Monocotyledons. Malayan Nature Society, Kuala Lumpur 
    4. Ketaran S (1988) Pengantar Teknologi Minyak Atsiri. Balai Pustaka, Jakarta 
    5. Kumar A, Dutta GP (1987) Indigenous plants oils as larvicidal agent against Anopheles stephensi mosquitoes. Curr Sci 54: 959-960 
    6. Burkill IH (1966) A Dictionary of the Economic Products of the Malay Peninsula, Vol 1. Ministry of Agriculture and Co., Kuala Lumpur, pp 734-738 
    7. Hudson TH, Kester DE, Davies FT, Geneve RL (1997) Plant Propagation: Principles and Practices, Ed 6. PrenticeHall, New Jersey 
    8. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473-497 
    9. Winton LL, Verhagen SA (1977) Douglas-fir shoots cultures. Can J Bot 55: 1246-1250 
    10. Akhila A (1986) Biosynthesis of monoterpenes in Cymbopagan winterianus. Phytochem 25: 421-424 
  • 이 논문을 인용한 문헌 (3)

    1. 2006. "" Journal of plant biology = 식물학회지, 49(3): 193~199     
    2. 2006. "" Journal of plant biology = 식물학회지, 49(4): 267~270     
    3. 2007. "" Journal of plant biology = 식물학회지, 50(4): 440~446     

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