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흐름주입식 바이오센서용 기질용액 자동희석 장치 개발
Development of an Auto Dilution Unit of Substrate Solutionfor a Flow Injection Type Biosensor

송대빈   (Division of Agricultural Engineering, Gyeongsang National University, Institute of Agricultural & Life ScienceUU0000114  ); 정효석   (Division of Agricultural Engineering, Gyeongsang National UniversityUU0000114  ); 정대홍   (Division of Agricultural Engineering, Gyeongsang National UniversityUU0000114  ); 김성태   (Division of Agricultural Engineering, Gyeongsang National University, Institute of Agricultural & Life ScienceUU0000114  );
  • 초록

    For development of an on-line monitoring unit of fermentation process, an auto dilution unit based on traditional chemical and biological analytical method was developed and the performance was evaluated. The dilution unit was constructed with two syringe pumps and flow direction change valves and fully automated. Total delivery volume of two pumps using distilled water was measured to confirm the operating stability And diluted concentrations of three substrate solutions (glucose, lactic acid, ethanol) were compared with a standard method with a high performance liquid chromatograph (glucose, lactic acid) and gas chromatograph (ethanol). Relative error values of total delivery volume of the pumps were below 3% and standard deviation values were 0.003 (n=5). Relative error values of diluted concentration of the dilution unit measurements were below 2% with 1/10 of dilution ratio and 70, $80{\mu}{\ell}$ of sample volume conditions for glucose and lactic acid, 1/30 of dilution ratio and $70{\mu}{\ell}$ of sample volume conditions for ethanol, respectively. In case of the ethanol, cause of the evaporative characteristics, the relative error values showed over 5% whole experimental conditions.


  • 주제어

    Biosensor .   FIA .   Auto dilution .   Fermentation .   On-line monitoring.  

  • 참고문헌 (4)

    1. Hansen, E. H., J. Ruzicka, F. J. Krug and E. A. G. Zagatto. 1983. Selectivity in flow injection analysis. Analytica Chimica Acta. 148:111-125 
    2. Olsen, S., J. Ruzicka and E. H. Hansen. 1982. Gradient techniques in flow injection analysis: Stoped-flow measurement of the activity of lactate dehydrogenase with electronic dilution. Analytica Chimica Acta. 136: 101-112 
    3. Ruzicka, J. and E. H. Hansen. 1978. Flow injection analysis: part X. theory, techniques and trends. Analytica Chimica Acta. 99:37-76 
    4. Schuhmann, W., H. Wohlschlager, J. Huber, H. L. Schmidt and H. Stadler. 1995. Development of an extremely flexible automatic analyzer with integrated biosensors for on-line control of fermentation processes. Analytica Chimica Acta 315:113-122 

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