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Helicobacter pylori에서 생성되는 Vacuolating Cytotoxin이 위상피세포에서 Eotaxin 발현에 미치는 영향
Expression of Eotaxin in Gastric Epithelial Cells Stimulated with Helicobacter pylori Vacuolating Cytotoxin

박효간    (한양대학교 의과대학 미생물학교실   ); 김남인    (한양대학교 의과대학 미생물학교실   ); 김주성    (서울대학교 의과대학 내과학교실 및 간연구소   ); 오유경    (고려대학교 생명과학부   ); 김영전    (중부대학교 생명공학부   ); 김나영    (서울대학교 의과대학 내과학교실 및 간연구소   ); 정현채    (서울대학교 의과대학 내과학교실 및 간연구소   ); 송인성    (서울대학교 의과대학 내과학교실 및 간연구소   ); 김정목    (한양대학교 의과대학 미생물학교실  );
  • 초록

    Helicobacter pylori-infected gastric mucosa is characterized by infiltration of various inflammatory cells such as neutrophils and eosinophils. Although several mechanisms for neutrophil infiltration are well known, there has been little known the role of eotaxin, which is a potent chemoattractant for eosinophils, on the inflammatory process of H. pylori infection. The present study was to investigate the mechanisms of eotaxin expression in gastric epithelial cells stimulated with H. pylori vacuolating cytotoxin (VacA). Stimulation with VacA purified from $VacA^+$ H. pylori slightly increased eotaxin expression in MKN-45 gastric epithelial cells. In contrast, the combined stimulation with VacA and IL-4 synergistically increased the eotaxin expression as determined by quantitative RT-PCR and ELISA. In MKN-45 cells transfected with an eotaxin promoter-luciferase reporter plasmid, co stimulation with VacA and IL-4 induced more luciferase activity than either VacA or IL-4 alone did. However, such up-regulation was significantly decreased in the cells transfected with luciferase reporter plasmid bearing an eotaxin promoter which has a mutation at STAT6 binding site. These results suggest that the up-regulation of eotaxin in VacA-stimulated gastric epithelial cells may be synergistically facilitated by IL-4 via a STAT6-dependent mechanism.


  • 주제어

    Helicobacter pylori vacuolating cytotoxin .   Eotaxin .   STAT6.  

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