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Journal of bacteriology and virology : JBV v.36 no.1, 2006년, pp.21 - 30   피인용횟수: 1

다약제 내성 Acinetobacter 균의 유전증 결정 및 항균성 내성의 특성 규명
Determination of Genomospecies and Characterization of Antimicrobial Resistance of Multi-drug Resistant Acinetobacter spp. Isolates

임유미    (경북대학교 의과대학 미생물학교실   ); 최태생    (단국대학교 의과대학 미생물학교실   ); 김정민    (경북대학교 의과대학 미생물학교실  );
  • 초록

    Acinetobacter species are non-fermentative Gram-negative coccobacilli and they have emerged as important nosocomial pathogens which are associated with the significant multidrug resistance in recent years. Carbapenem-resistant A. baumannii (CRAB) and pandrug-resistant A. baumannii (PDRAB) were reported in 1991 and 1998, respectively. Fifty-eight isolates of Acinetobacter species recovered from a university hospital between August 2004 and March 2005 were investigated for the existence of CRAB, PDRAB, extended-spectrum ${\beta}$ -lactamase (ESBL)-producing Acinetobacter and examined for their phenotypic and genotypic characteristics. Genomospecies of Acinetobacter species were determined by amplified rDNA restriction analysis (ARDRA) and antimicrobial susceptibility test was performed with 13 kinds of antimicrobial agents. Metallo- ${\beta}$ -lactamase (MBL) producers were screened by modified hodge test and confirmed by imipenem-EDTA disk synergy test. Detection of $bla_{IMP-1},\;bla_{VIM-2},\;bla_{TEM},\;and\;bla_{PER-1}$ was performed by PCR. Genomic DNAs were analyzed by pulsed-field gel electrophoresis (PFGE). Among 58 isolates of Acinteobacter species, 40 isolates were identified as genospecies 2 (A. baumannii), 9 were 13TU, 5 were A. phenon 6/ct, and 4 were Acinetobacter genospecies 3 by ARDRA. Thirteen isolates were confirmed as MBL-producers and $bla_{IMP-1}\;and\;bla_{VIM-2}$ were carried by 5 and 8 isolates of them, respectively. MBL-producers were mostly 13TU, A. phenon 6/ct 13TU, and Acinetobacter genospecies 3 and they were susceptible to ciprofloxacin and ampicillin-sulbactam. $Bla_{PER-1}$ was carried by thirteen isolates and 12 isolates of them were PDRAB showing resistance to all antimicrobial agents tested, including ceftazidime, cefepime, aztreonam, ciprofloxacin, amikacin, gentamicin, ampicillin-sulbactam, and imipenem. In conclusion, most MBL-producers belonged to 13TU, A. phenon 6/ct 13TU, and Acinetobacter genospecies 3 which were susceptible to ciprofloxacin and ampicillin-sulbactam, whereas 12 of 13 PER-1-producers were PDRAB originated from the same clone.


  • 주제어

    Acinetobacter spp .   Genomic typing .   Metallo- ${\beta}$-lactamase .   PER-1 .   Pan-drug resistant Acinetobacter baumannii.  

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  • 이 논문을 인용한 문헌 (1)

    1. Rhee, Moon-Soo ; Kim, Gwan-Pil ; Bang, Byung-Ho 2010. "Screening and Optimal Culture Conditions of Antibiotic-Producing Actinomycetes B-51 for Multidrug Resistant Acinetobacter baumannii" 한국식품영양학회지 = The Korean journal of food and nutrition, 23(1): 63~69     

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