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닭 분변유래 Enterococcus spp.의 Fluoroquinolone계 내성관련 gyrA 및 parC Gene의 변이
Alteration in gyrA and parC Gene Associated with Fluoroquinolone Resistance of Enterococcus spp. Isolated from Feces of Chicken

조재근    (대구광역시 보건환경연구원   ); 김기석    (경북대학교 수의과대학   ); 박청규    (경북대학교 수의과대학   ); 곽동미    (경북대학교 수의과대학   ); 김애란    (국립수의과학검역원   ); 강민수    (국립수의과학검역원   ); 김종완    (국립수의과학검역원   ); 김병한    (국립수의과학검역원   ); 구복경    (국립수의과학검역원   ); 이영주    (경북대학교 수의과대학  );
  • 초록

    The purpose of this study was to investigate the fluoroquinolone resistance frequency of Enterococcus spp. from normal chicken feces and to analyse mutations of the gyrA and parC gene associated with fluoroquinolone resistance. Among 52 Enterococcus faecalis and 25 E. faecium isolates, 23 (44.2%) E. faecalis and 7 (28.0%) E. faecium were resistant to ciprofloxacin (CIP) by disc diffusion method. Genetic exchange in gyrA and parC gene among 2 CIP intermediate isolates and 15 CIP resistant isolates were found in the amino acid codon of Ser-83 and Asp-87, and Ser-80 and Glu-84, respectively. These mutants contained a change from Ser to Phe, Val, Tyr, Ile, Thr or Pro at codon 83 and from Glu to Gly or Leu at codon 87 in gyrA gene, and a change from Ser to Ile or Thr at codon 80 and from Glu to Asp or Lys at codon 84 in parC gene. The isolates with mutation in gyrA regardless of a mutation in parC showed high resistance ( $MIC{\ge}232{\mu}g/ml$ ) to CIP, enrofloxacin, norfloxacin and ofloxacin. These results suggested that gyrA gene is the primary target for 4 fluoroquinolones resistance in Enterococcus spp.


  • 주제어

    Enterococcus .   fluoroquinolone .   gyrA .   parC.  

  • 참고문헌 (26)

    1. Ferrero L, Cameron B, Crouzet J: Analysis of gyrA and grlA mutations in stepwise-selected ciprofloxacin-resistant mutants of Staphylococcus aureus. Antimicrob Agents Chemother 39: 1554-1558, 1995 
    2. Garau J, Xercavins M, Rodriguez-Carballeira M, GomezVera JR, Coil I, Vidal D, Llovet T, Ruiz-Bremon A: Emergence and dissemination of quinolone-resistant Escherichia coli in the community. Antimicrob Agents Chemother 43: 2736-2741, 1999 
    3. Hooper DC, Wolfson JS: Fluoroquinolone antimicrobial agents. N Engl J Med 324: 384-394, 1991 
    4. Morrison AJ Jr, Wenzel RP: Nosocomial urinary tract infections due to Enterococcus Ten years experience at a university hospital. Arch Intern Med 146: 1549-1551, 1986 
    5. Onodera Y, Okuda J, Tanaka M, Sato K: Inhibitory activi- . ties of quinolones against DNA gyrase and topoisomerase IV of Enterococcus faecalis. Antimicrob Agents Chemother 46: 1800-1804, 2002 
    6. Bazile-Pham-Khac S, Truong QC, Lafont JP, Gutmann L, Zhou XY, Osman M, Moreau NJ: Resistance to fluoroquinolones in Escherichia coli isolated from poultry. Antimicrob Agents Chemother 40: 1504-1507, 1996 
    7. 송시욱, 정석찬, 김성일, 정명은, 김계희,이지연, 임숙경, 이영주, 조남인, 박종명, 박용호: 2003년도 국내 도축장에서 분리한 세균의 항생제 감수성 조사 1. 도축장의 식육으로부터 분리한 E. coli의 항생제 감수성. 한국수의공중보건학회지 28: 215-221, 2004 
    8. del Mar LIeo M, Taft MC, Signoretto C, Dal Cero C, Canepari P: Competitive polymerase chain reaction for quantification of nonculturable Enterococcus faecalis cells in lake water. FEMS Microbial Eco 130: 345-353, 1999 
    9. Gellert M, Mizuuchi K, O'Dea MH, Nash HA: DNA gyrase: an enzyme that introduces superhelical turns into DNA. Proc Natl Acad Sci USA 73: 3872-3876, 1976 
    10. Korten V, Huang WM, Murray BE: Analysis by PCR and direct DNA sequencing of gyrA mutations associated with fluoroquinolone resistance in Enterococcus faecalis. Antimicrab Agents Chemother 38: 2091-2094, 1994 
    11. Kanematsu E, Deguchi T, Yasuda M, Kawamura T, Nishino Y, Kawada Y: Alterations in the gyrA subunit of DNA gyrase and the parC subunit of DNA topoisomerase IV associated with quinolone resistance in Enterococcus faecalis. Antimicrob Agents Chemother 42: 433-435, 1998 
    12. Murray BE: The life and times of the Enterococcus. Clin Microbiol Rev 3: 46-65, 1990 
    13. Ferrero L, Cameron B, Manse B, Lagneaux D, Crouzet J, Famechon A, Blanche F: Cloning and primary structure of Staphylococcus aureus DNA topoisomerase IV: a primary target of fluoroquinolones. Mol Microbiol 13: 641-653, 1994 
    14. Kato J, Nishimura Y, Imamura R, Niki H, Hiraga S, Suzuki H: New topoisomerase essential for chromosome segregation in Escherichia coli. Cell 63: 393-404, 1990 
    15. Cheng S, McCleskey FK, Gress MJ, Petroziello JM, Liu R, Namdari H, Beninga K, Salmen A, DelVecchio VG: A PCR assay for identification of Enterococcusfaecium. J Clin Microbiol 35: 1248-1250, 1997 
    16. Sylvain B, Fluit AC, Wagner U, Heisig P, Milatovic D, Verhoef J, Scheuring S, Kohrer K, Schmitz FJ: Association of alteration in parf' and gyrA proteins with resistance of clinical isolates of Enterococcus faecium to nine different fluoroquinolones. Antimicrob Agents Chemother 43: 2513-2516, 1999 
    17. Chaslus-Dancla E, Martel JL, Carlier C, Lafont JP, Courvalin P: Emergence of aminoglycoside 3-N-acetyltransferase IV in Escherichia coli and Salmonella typhimurium isolated from animals in France. Antimicrob Agents Chemother 29: 239-243, 1986 
    18. Pitout JD, Thomson KS, Hanson ND, Ehrhardt AF, Coudron P, Sanders CC: Plasmid-mediated resistance to expandedspectnun cephalosporins among Enterobacter aerogenes strains. Antimicrob Agents Chemother 42: 596-600, 1998 
    19. el Amin NA, Jalal S, Wretlind B. Alterations in gyrA and parC associated with fluoroquinolone resistance in Enterococcus faecium. Antimicrob Agents Chemother 43: 947-949, 1999 
    20. Endtz HP, Ruijs GJ, van Klingeren B, Jansen WH, van der Reyden T, Mouton RP: Quinolone resistance in Campylobacter isolated from man and poultry following the introduction of fluoroquinolones in veterinary medicine. J Antimcirob Chemother 27: 199-208, 1991 
    21. Tankovic J, Perichon B, Duval J, Courvalin P: Contribution of mutations in gyrA and parC genes to fluoroquinolone resistance of mutants of Streptococcus pneumoniae obtained in vivo and in vitro. Antimicrob Agents Chemother 40: 2505-2510, 1996 
    22. Na EY, Trucksis M, Hopper DC: Quinolone resistance mutations in topoisomerase IV: relationship to the flqA locus and genetic evidence that topoisomerase IV is the primary target and DNA gyrase is the secondary target of fluoroquinolones in Staphylococcus aureus. Antimicrob Agents Chemother 40: 1881-1888, 1996 
    23. 이영주, 김애란, 정석찬, 송시욱, 김재홍: 닭 분변유래 E. coli 및 Salmonella spp.의 항생제 내성패턴. 대한수의학회지 45: 75-83, 2005 
    24. Pan XS, Ambler J, Mehtar S, Fisher LM: Involvement of topoisomerase IV and DNA gyrase as ciprofloxacin targets in Streptococcus pneumoniase. Antimicrob Agents Chemother 40: 2321-2326, 1994 
    25. Pan XS, Fisher LM: Targeting of DNA gyrase in Streptococcus pneumoniae by sparfioxacin: selective targeting of gyrase or topoisomerase IV by quinolones. Antimicrob Agents Chemother 41: 471-474, 1997 
    26. Tankovic J, Mahjoubi F, Courvalin P, Duval J, Leclercq R: Development of fluoroquinolone resistance in Enterococcus faecalis and role of mutations in the DNA gyrase gyrA gene. Antimicrob Agents Chemother 40: 2558-2561, 1996 

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