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Coxsackievirus B3 H3와 10A1 감염에 의한 마우스 심장 내에서의 유전자 변이 관찰
Host Gene Profiling of Coxsackievirus B3 H3- and 10A1-infected Mouse Heart

남재환    (가톨릭대학교 생명공학부 분자바이러스연구실   ); 임병관    (성균관대학교 의과대학 순환기내과   ); 조영주    (가톨릭대학교 생명공학부 분자바이러스연구실   ); 김대선    (가톨릭대학교 생명공학부 분자바이러스연구실   ); 김연정    (가톨릭대학교 생명공학부 분자바이러스연구실   ); 정수영    (가톨릭대학교 생명공학부 분자바이러스연구실   ); 지영미    (국립보건연구원 간염 폴리오 연구팀   ); 전은석    (성균관대학교 의과대학 순환기내과  );
  • 초록

    Coxsackievirus B3 (CVB3) is a non-enveloped virus that has a single-stranded RNA genome. CVB3 induces myocarditis, and ultimately, dilated cardiomyopathy. A myocarditis variant of CVB3 (CVB3 H3) and its antibody-escape mutant (CVB3 10A1) were studied previously; H3 was found to induce myocarditis and 10A1 was found to be attenuated in infected mice. Although amino acid residue 165, located in a puff region of VP2, was found to be altered (i.e., the H3 asparagine was altered to aspartate in 10A1), the detailed mechanism of attenuation was not clearly elucidated. Here, DNA microarray technology was used to monitor changes in mRNA levels of infected mouse hearts after CVB3 H3 and 10A1 infection. This tool was used to elucidate the pathogenic mechanisms of viral infection by understanding virus-host interactions. We identified several genes, including protein tyrosine kinases, Ddr2 and Ptk2, as well as Clqb and Crry, involved in complement reactions, which may be involved in these viral processes. Thus, gene profiling can provide an opportunity to understand host immune responses to viral infection for gene therapy and may contribute to the identification of the target gene that is modified during treatment of viral myocarditis.


  • 주제어

    Coxsackievirus B3 H3 .   10A1 .   cDNA microarray .   Gene profile.  

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