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Newcastle Disease Virus의 F와 HN 유전자 재조합 DNA 제조 및 면역원성
Construction of Recombinant DNA with F and HN Genes of Newcastle Disease Virus and Its Immunogenicity

김지영    (충남대학교 수의과대학   ); 초가기    (충남대학교 수의과대학   ); 박종현    (국립수의과검역원   ); 서상희    (충남대학교 수의과대학   ); 박창식    (충남대학교 형질전환복제돼지연구센터   ); 김명철    (충남대학교 수의과대학   ); 전무형    (충남대학교 수의과대학  );
  • 초록

    Recombinant DNA vaccines, based on plasmid vectors expressing an antigen under the control of a strong promotor, have several advantages over traditional vaccines. They have been shown to induce a full spectrum of immune responses for humoral and cellular systems and to secure the higher safety and the simplicity of administration. Thus, establishment of DNA vaccines against Newcastle disease virus (NDV) in poultry has been widely investigated using various virus strains and vector systems. In this study, the F and HN genes of NDV CBP-1 strains isolated from diseased pheasants and attenuated by serial passages in egg embryos were cloned using pSLIA vector and constructed two recombinants of pSLIA-tsF and pSLIA-tsHN. The recombinant plasmids were transfected into COS-7 cell and the expression of HN and F proteins were verified by immunofluorescence, SDS-PAGE and Western blot. The recombinant plasmids were injected intramuscularly and intradermally into C57B/6 mouse and a significant increment of HN and F antibodies was detected by ELISA. According to the results, it was implicative that the recombinant DNA could be utilized for development of recombinant DNA vaccine for NDV.

  • 주제어

    NDV vaccine .   F and HN gene .   Recombinant NDV vaccine.  

  • 참고문헌 (35)

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