본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

탄저균 스턴 아포에 대한 대식세포에서의 TNF-${\alpha}$와 IL-12의 분비
TNF-${\alpha}$ and IL-12 Secretion in Macrophages in Response to Spores of Bacillus anthracis Sterne

백진주    (한양대학교 과학기술대학 분자생명과학부   ); 정경화    (한양대학교 과학기술대학 분자생명과학부   ); 채영규    (한양대학교 과학기술대학 분자생명과학부  );
  • 초록

    Bacillus anthracis is a gram-positive, aerobic, spore forming, and rod-shaped bacterium. Anthrax spores are introduced into macrophage by phagocytosis and multiply after germination. The anthrax spores infected in macrophage produce lethal toxin eventually caused cell death. In this study, we analyzed apoptosis and cytokine TNF- ${\alpha}$ and IL-12 secretion after the infection of spores of B. anthracis Sterne in the murine macrophage RAW264.7 cells and in the primary human macrophages. In murine macrophage RAW264.7 cells infected by spore of B. anthracis Sterne, the cells were markedly changed in secretion of TNF- ${\alpha}$ ( $482{\sim}6,213pg/ml$ ) by lethal toxin, and induced apoptosis. In case of RAW264.7 cells infected by formalin-inactivated spores of B. anthracis, the cells were not able to produce lethal toxin, which released lower level concentration of TNF- ${\alpha}$ ( $7.7{\sim}97.2pg/ml$ ), and rarely induced apoptosis. When primary human macrophage cells infected with spores of B. anthracis Sterne, they secreted TNF- ${\alpha}$ ( $5{\sim}16pg/ml$ ), and induced apoptosis about 1% of total cells. We presented that inducing apoptosis by spores of B. anthracis Sterne capable of expressing lethal toxin is related with the secretion of TNF- ${\alpha}$ in murine macrophage RAW264.7 cells. These studies revealed that human and murine macrophages has affected differently by anthrax lethal toxin produced by spores of B. anthracis Sterne.


  • 주제어

    Bacillus anthracis Sterne .   Spores .   Murine macrophage RAW264.   7 cells .   Human macrophage .   Tumor necrosis factor alpha (TNF- ${\alpha}$) .   Interleukin-12.  

  • 참고문헌 (35)

    1. Fritz DL, Jaax NK, Lawrence WB, Davis KJ, Pitt MLM, Ezzell J, Friedlander A: Pathology of experimental inhalation anthrax in the rhesus monkey. Lab Investig 73: 691-702, 1995 
    2. Hanna PC, Acosta D, Collier RJ: On the role of macrophages in anthrax. Proc Natl Acad Sci USA 90: 10198-10200, 1993 
    3. Kim SJ, Cho KS, Choi YK, Chai YG: Production of Bacillus anthracis protective antigen by improvement of culture condition and purification methods. Kor J Microbiol 37: 1-27, 2001 
    4. Krantz BA, Melnyk RA, Zhang S, Juris SJ, Lacy DB, Wu Z, Finkelstein A, Collier RJ: A phenylalanine clamp catalyzes protein translocation through the anthrax toxin pore. Science 309: 777-81, 2005 
    5. Klimpel KR, Arora N, Leppla, SH: Anthrax toxin lethal factor contains a zinc metalloprotease consensus sequence which is required for lethal toxin activity. Mol Microbiol 13: 1093-100, 1994 
    6. McHugh SL, Yamamoto Y, Klein TW, Friedman H: Murine macrophages differentially produce proinflammatory cytokines after infection with virulent vs. avirulent Legionella pneuphila. J Exp Med 190: 953-962, 2000 
    7. Popov SG, Villasmil R, Bernardi J, Grene E, Cardwell J, Popova T, Wu A, Alibek D, Bailey C, Alibek K: Effect of Bacillus anthracis lethal toxin on human peripheral blood mononuclear cells. FEBS Lett 527: 211-215, 2002 
    8. Smith H: Discovery of the anthrax toxin: the beginning of in vivo studies on pathogenic bacteria. Trends Microbiol 8: 199 -200, 2000 
    9. Wallach D: Cell death induction by TNF: a matter of self control. Trends Biochem Sci 22: 107-109, 1997 
    10. Ma X: TNF-alpha and IL-12: a balancing act in macrophage functioning. Microbes Infect 3: 121-129, 2001 
    11. Popov SG, Popova TG, Grene E, Klotz F, Cardwell J, Bradburne C, Jama Y, Maland M, Wells J, Nalca A, Voss T, Bailey C, Alibek K: Systemic cytokine response in murine anthrax. Cell Microbiol 6: 225-233, 2004 
    12. Tonello F, Ascenzi P, Montecucco C: The metalloproteolytic activity of the anthrax lethal factor is substrate-inhibited. J Biol Chem 274: 40075-40078, 2003 
    13. Moayeri B, Haines D, Young HA, Leppla SH: Bacillus anthracis lethal toxin induces TNF-$\alpha$-independent hypoxiamediated toxicity in mice. J Clin Invest 112: 670-682, 2003 
    14. Beauregard, KE, Collier RJ, Swanson JA: Proteolytic activation of receptor-bound anthrax protective antigen on macrophages promotes its internalization. Cell Microbiol 2: 251-258, 2000 
    15. Guidi-Rontani C, Mock M: Macrophage interactions. Curr Top Microbiol Immunol 271: 115-141, 2002 
    16. Klein F, Walker JS, Fitzpatrick DF, Lincoln RE, Mahlandt BG, Jones WIJ, Dobbs JP, Hendrix KJ: Pathophysiology of anthrax. J Infect Dis 116: 123-138, 1966 
    17. Erwin JL, DaSilva LM, Bavari S, Little SF, Friedlander AM, Chanh TC: Macrophage-derived cell lines do not express proinflammatory cytokines after exposure to Bacillus anthracis lethal toxin. Infect Immun 69: 1175-1177, 2001 
    18. Lacy DB, Collier RJ: Structure and function of anthrax toxin. Curr Top Microbiol Immunol 271: 61-85, 2002 
    19. Wewers MD: IL-1 beta: an endosomal exit. Proc Natl Acad Sci USA 101: 10241-10242, 2004 
    20. Cordoba-Rodriguez R, Fang H, Lankford CS, Frucht DM: Anthrax lethal toxin rapidly activates caspase-1/ICE and induces extracellular release of interleukin (IL)-1beta and IL-18. J Biol Chem 279: 20563-20566, 2004 
    21. Dragon DC, Rennie RP: Evaluation of spore extraction and purification methods for selective recovery of viable Bacillus anthracis spores. Lett Appl Microbiol 33: 100-105, 2001 
    22. Agrawal A, Lingappa J, Leppla SH, Agrawal S, Jabbar A, Quinn C, Pulendran B: Impairment of dendritic cells and adaptive immunity by anthrax lethal toxin. Nature 424: 329 -334, 2003 
    23. Mock M, Fouet A: Anthrax. Annu Rev Microbiol 55: 647 -671, 2001 
    24. Seo, GM, Kim SJ, Chai YG: Rapid profiling of the infection of Bacillus anthracis on human macrophages using SELDITOF mas spectroscopy. Biochem Biophys Res Commun 325: 1236-1239, 2004 
    25. Tracey KJ, Fong Y, Hesse DG, Manogue KR, Lee AT, Kuo GC, Lowry SF, Cerami A: Anti-cachectin/TNF monoclonal antibodies prevent septic shock during lethal bacteraemia. Nature 330: 662-664, 1987 
    26. Cerrtti D, Hollingsworth LT, Kozolosky CJ, Valentine MB, Shaprio DN, Morris SW, Nelson N: Molecular characterization of the gene for human interleukin-1-beta converting enzyme (IL1BC). Genomics 20: 468-473, 1994 
    27. Jung KH, Seo GM, Kim SJ, Kim JC, Oh SM, Oh KG, Chai YG: Proteome profiling of murine macrophages treated with the anthrax lethal toxin. Kor J Microbiol 41: 262-168, 2005     
    28. Chopra AP, Boone SA, Liang X, Duesbery NA: Anthrax lethal factor proteolysis and inactivation of MAPK kinase. J Biol Chem 278: 9402-9406, 2003 
    29. Vitale G, Bernardi L, Napolitani G, Mock M, Montecucco C: Susceptibility of mitogen-activated protein kinase kinase family members to proteolysis by anthrax lethal factor. Biochem J 352: 739-745, 2000 
    30. Zaucha GM, Pitt LM, Estep J, Ivins BE, Friedlander A: The pathology of experimental anthrax in rabbits exposed by inhalation and subcutaneous inoculation. Arch Pathol Lab Med 122: 982-992, 1998 
    31. Milne JC, Furlong D, Hanna PC, Collier RJ: Anthrax protective antigen forms oligomers during intoxication of mammalian cells. J Biol Chem 269: 20607-20612, 1994 
    32. Pickering AK, Merkel TJ: Macrophages release tumor necrosis factor alpha and interleukin-12 in response to intracellular Bacillus anthracis spores. Infect Immun 72: 3069 -3072, 2004 
    33. Smith H, Stoner HB: Anthrax toxic complex. Fed Proc 26: 1554-1557, 1967 
    34. Fouet A, Mesnage S: Bacillus anthracis cell envelope components. Curr Top Microbiol Immunol 271: 87-113, 2002 
    35. Pickering AK, Osorio M, Lee GM, Grippe VK, Bray M, Merkel TJ: Cytokine response to infection with Bacillus anthracis spores. Infect Immun 72: 6382-6389, 2004 

 저자의 다른 논문

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기