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Journal of bacteriology and virology : JBV v.36 no.3, 2006년, pp.185 - 194   피인용횟수: 1

국내 돼지에서 분리한 내인성 레트로 바이러스 gag 유전자의 분자 생물학적 특성 분석
Molecular Characterization of Porcine Endogenous Retrovirus gag Genes from Pigs in Korea

이정은    (건국대학교 동물생명과학대학 동물생명공학과   ); 이동희    (건국대학교 동물생명과학대학 동물생명공학과   ); 유재영    (건국대학교 동물생명과학대학 동물생명공학과   ); 김계웅    (공주대학교 산업과학대학 동물자원학과   ); 박홍양    (건국대학교 동물생명과학대학 동물생명공학과   ); 이훈택    (건국대학교 동물생명과학대학 동물생명공학과   ); 김영봉    (건국대학교 동물생명과학대학 동물생명공학과  );
  • 초록

    Xenotransplantation, as a potential solution to the shortage of human organs, is associated with a number of concerns including immunologic rejection and xenogenic infection. While the pigs are considered the most suitable organ source for xenotransplantation, there is a potential public health risk due to zoonosis. Among the known porcine zoonotic microbes, Porcine Endogenous Retrovirus (PERV) is the most considerable virus. PERV belongs to the Gammaretrovirus and has been divided into three groups (A, B, and C). To characterize the gag of PERVs, we isolated the genomic DNAs from three pig breeds (Birkshire, Duroc, and Yorkshire) and two types of SPF miniature pigs. About 1.5 kb fragments covering full length of gag were amplified and cloned into T-vector. A total of 38 clones were obtained and sequenced. Nucleotide sequences were analyzed and phylogenetic trees were constructed from the nucleotide and deduced amino acids. PERV-A, -B and -C were present in the proportion of 47, 19 and 34%, respectively. Regardless of origin or subgroups, gag clones showed highly homology in nucleotide and deduced amino acid sequences. Deduced amino acids sequence alignments showed typical conserve sequences, Cys-His box and processing sites. Among analyzed clones, about 28% of isolates had the correct open reading frame. To test the functional expression of Gag protein, gag was subcloned into expression vector and confirmed its expression in HeLa cell. This research provides the fundamental information about molecular characteristics of gag gene and functional Gag protein related xenotropic PERVs.


  • 주제어

    PERV .   Xenotransplantation .   Group specific antigen .   Zoonosis.  

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  • 이 논문을 인용한 문헌 (1)

    1. Lee, D.H. ; Lee, J.E. ; Kim, H.M. ; Kim, G.W. ; Park, H.Y. ; Kim, Young-Bong 2007. "Detection and Classification of Porcine Endogenous Retroviruses by Polymerase Chain Reaction" 한국동물자원과학회지 = Journal of animal science and technology, 49(3): 405~414     

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