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Journal of bacteriology and virology : JBV v.36 no.4, 2006년, pp.221 - 228  
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Sucrose 발효 및 비발효 Vibrio vulnificus 균주의 생물학적 및 유전학적 성상 비교
Comparison of Biological and Genetic Characteristics Between Sucrose-Fermenting and Sucrose-Nonfermenting Vibrio vulnificus Isolates

김신무    (원광보건대학 임상병리과   ); 소향아    (전북보건환경연구원 미생물부   ); 송계민    (순천대학교 대학원 생물학과   ); 이영엽    (전주대학교 대학원 생물학과   ); 임채원    (원광보건대학 임상병리과   ); 이재형    (원광대학교 의과대학 전정와우 연구센터   ); 소홍섭    (원광대학교 의과대학 전정와우 연구센터   ); 김진경    (원광대학교 의과대학 전정와우 연구센터   ); 박래길    (원광대학교 의과대학 전정와우 연구센터   ); 박석돈    (원광대학교 의과대학 피부과교실  );
  • 초록

    Twelve strains of V. vulnificus isolated from clinical specimens in $2002{\sim}2004$ in Jeollado province were determined for their biologic groups, serotypes, presence of vvhA (hemolysin/cytolysin) gene, DNA sequence, and PFGE patterns of NotI-restricted genomic DNA. The following results were obtained. All 12 isolates were biogroup 1, and API 20E profiles were: 5146105 for 5 (41.7%) isolates, and 5148125 for 2 isolates with sucrose fermentation. Ten (83.3%) of the 12 isolates was V. vulnificus serotype O4A, and two sucrose-fermenting isolates belonged to serotype O2. Alleles of cytolysin-hemolysin gene were detected in all 12 isolates. The nucleotide sequences of vvhA genes from strains WKHC 212 and WKHC 221 showed $94{\sim}97%$ similarity compared with those from previously reported 7 strains, YJ016, CMCP6, L-180, CDC B3547, IF Vv10, CIP 75.4T and CNRVC 970121. PFGE of NotI-restricted genomic DNA from the 12 isolates showed approximately 48.5 to 873-kb fragments and they were clustered to five (A to E) patterns. Two sucrose-fermenting isolates belonged to pattern D with 95% similarity with each other. Two strains isolated from two different patients had two identical patterns C and D. It is concluded that sucrose-fermenting strains also exist among clinical isolates of V. vulnificus in Korea, and they can be identified by using API 20E system, and by detecting vvhA gene. DNA sequences and PFGE pattern of NotI-restricted genomic DNA suggested that the two sucrose-fermenting isolates belonged to an identical clone, and two strains each isolated from two different patients belonged to two identical clones.


  • 주제어

    Sucrose-fermenting .   Vibrio vulnifcius .   Biological .   Genetic characteristic.  

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