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Journal of bacteriology and virology : JBV v.36 no.4, 2006년, pp.229 - 235  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Platelet Activating Factor Acetylhydrolase의 대량 생산법 개발
Development of a Mass Production Method of Platelet Activating Factor Acetylhydrolase

정영화    (영남대학교 의과대학 미생물학교실   ); 장현욱    (영남대학교 약학대학   ); 이태윤    (영남대학교 의과대학 미생물학교실  );
  • 초록

    Platelet activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a potent lipid mediator in a variety of physiological events. PAF is also involved in various pathological events including allergy and inflammation. PAF-acetylhydrolase (PAF-AH) hydrolyzes PAF to produce inactive lyso-PAF. Thus, overproduction of PAF-AF will be useful for the therapeutic valuation of the enzyme. In this study, we established an overproduction method of bovine PAF-AH in Escherichia coli system. We used bovine mammary gland for cDNA cloning. The cDNA had two mismatches of amino acid sequences (Thr-247 to Met and Ile-431 to Thr) compared with the previously reported PAF-AH cDNA (bovine spleen, NM_174578). The recombinant PAF-AH of 43 kDa in molecular size reacted with human PAF-AH polyclonal antibody and showed a strong PAF-AH enzyme activity in an in vitro assay system. The recombinant PAF-AH produced by this study can be applied for various experiments including in vivo models to test its protective activity against PAF-related diseases.


  • 주제어

    PAF-AH .   Anti-inflammatory protein .   Mass production.  

  • 참고문헌 (28)

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  • 이태윤 (14)

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