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Food science and biotechnology v.15 no.3 = no.69, 2006년, pp.392 - 398   피인용횟수: 5

Isolation and Characterization of Phytochemical Constituents from Soybean (Glycine max L. Merr.)

Lee, Jin-Hwan   (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDACC0077488  ); Baek, In-Youl   (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDACC0077488  ); Kang, Nam-Suk   (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDACC0077488  ); Ko, Jong-Min   (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDACC0077488  ); Han, Won-Young   (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDACC0077488  ); Kim, Hyun-Tae   (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDACC0077488  ); Oh, Ki-Won   (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDACC0077488  ); Suh, Duck-Yong   (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDACC0077488  ); Ha, Tae-Joung   (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDACC0077488  ); Park, Ki-Hun   (Division of Applied Life Science (BK21 program), Department of Agricultural Chemistry, Research Institute of Life Science, Gyeongsang National UniversityUU0000114  );
  • 초록

    Four flavonoids 1-4 and one phytosterol 5 were isolated from methanol extract of Taekwangkong, one of the soybean cultivars, and the structures of these compounds were fully characterized by physical and spectral analysis. The content of compounds 1-4 as determined by $C_{18}$ reversed phase HPLC (high-performance liquid chromatography) coupled with diode-array detector were 12.1, 624.6, 18.0, and $219.6\;{\mu}g/g$ , respectively, and the total phenolic content of this cultivar was measured as 3.7 mg gallic acid equivalent per g dry material (GAB/g). Also, compound 1 showed strong radical scavenging activity in the 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assay ( $IC_{50}\;=\;47.6\;{\mu}M$ ), five-fold higher than seen in the 1,1-diphenyl-2-picrylliydrazyl (DPPH) assay. These results lead to the conclusion that soybean not only has many phytoestrogens but also has potent antioxidant activity.


  • 주제어

    phytoestrogen .   Glycine max .   Taekwangkong .   HPLC .   total phenolic content .   ABTS.  

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  • 이 논문을 인용한 문헌 (5)

    1. 2007. "" Journal of applied biological chemistry, 50(2): 63~69     
    2. 2007. "" Food science and biotechnology, 16(6): 963~966     
    3. 2008. "" Food science and biotechnology, 17(3): 578~586     
    4. 2008. "" Journal of applied biological chemistry, 51(4): 172~175     
    5. 2009. "" Food science and biotechnology, 18(2): 488~493     

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