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덱사메타손이 생쥐 B세포의 세포 표면 인식자와 분화에 미치는 영향
Effect of Dexamethasone on the Surface Expression of Marker Molecules and Differentiation of Murine B Cells

여승근   (경희대학교 의과대학 이비인후과학교실UU0001575  ); 차창일   (경희대학교 의과대학 이비인후과학교실UU0001575  ); 박동춘   (가톨릭대학교 의과대학 산부인과학교실UU0000012  );
  • 초록

    Background: There are at least two different subsets of B cells, B-1 and B-2. The characteristic features and function of B-2 cells in addition to the effect of steroids on B-2 cells are well-known. Although B-1 cells have different features and functions from B-2 cells, the effect of steroids on B-1 cells is not completely understood. Therefore, this study examined the effects of dexamethasone on peritoneal (or B-1 cells) and splenic B cells (or B-2 cells). Methods: Purified B cells were obtained from the peritoneal fluid and the spleens of mice. The isolated B cells were cultured in a medium and after adding different concentrations of dexamaethasone. The cell survival rate was measured by flow cytometry using propidium iodide. The expression level of the B cell surface marker was analyzed by flow cytometry. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by an enzyme-linked immunosorbent assay. Results: The survival rate of peritoneal and splenic B cells decreased with increasing dexamethasone concentration. However, the rate of peritofieal B cell apoptosis was lower than that of splenic B cells. CDS and B7.1 expression in peritoneal B cells and CD23 and sIgM expression in splenic B cells after the dexamethasone treatment were reduced. When B cells were treated with dexamethasone, the spontaneous IgM secretion decreased with increasing dexamethasone concentration. Conclusion: Dexamethasone induces apoptosis in peritoneal and splenic B cells. However, peritoneal B cells are less sensitive to dexamethasone. The dexamethasone suppressed expression of the surface markers in peritoneal B cells is different from those in splenic B cells.


  • 주제어

    Dexamethasone .   peritoneal B cell .   splenic B cell.  

  • 참고문헌 (20)

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    2. 2006 "Differences in Their Proliferation and Differentiation between B-1 and B-2 Cell" Immune network : official journal of the Korean association of immunobiologists 6 (1): 1~5    
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  • 차창일 (5)

  • 박동춘 (1)

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