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Association of Killer Cell Ig-like Receptor (KIR) with an Adaptor Protein Shc

Cho, Hyun-Il   (Department of Microbiology, Yonsei University College of MedicineUU0000936  ); Chwae, Yong-Joon   (Department of Microbiology, Yonsei University College of MedicineUU0000936  ); Park, Sang-Myun   (Department of Microbiology, Yonsei University College of MedicineUU0000936  ); Kim, Jong-Sun   (Department of Microbiology, Yonsei University College of MedicineUU0000936  );
  • 초록

    Background: Cytotoxic function of killer cells is inhibited by specific recognition of class I MHC molecules on target cells by inhibitory killer Ig-like receptors (KIR) expressed on NK cells and some cytotoxic T cells. The inhibitory effect of KIR is accomplished by recruitment of SH2-containing protein tyrosine phosphatase (SHP) to the phosphotyrosine residues in the cytoplasmic tail. Methods: By in vitro coprecipitation experiments and transfection analysis, we investigated the association of KIR with an adaptor protein Shc in Jurkat T cells. Results: The cytoplasmic tail of KIR appeared to associate with an adaptor protein Shc in Jurkat T celilysates. Similar in vitro experiments showed that phosphorylated KIR cytoplasmic tail bound SHP-1 and Shc in Jurkat T cell lysates. The association of KIR with Shc was further confirmed by transfection analysis in 293T cells. Interestingly, however, Shc appeared to be replaced by SHP-2 upon engagement of KIR in 293T cells. Conclusion: Our data indicate that KIR associate with an adaptor protein Shc in Jurkat T cells, and suggest that KIR might have an additional role which is mediated by this adaptor protein.


  • 주제어

    KIR .   Shc .   SHP-1 .   SHP-2 .   cell activation .   cell proliferation .   inhibitory signal transduction.  

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