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실험적으로 항원에 의하여 일차 자극된 $CD4^+$ T 세포의 이차 면역 반응의 분석
The Kinetics of Secondary Response of Antigen-Specific $CD4^+$ T Cells Primed in vitro with Antigen

박성옥   (전북대학교 수의과대학 미생물학교실 및 생체안전성연구소UU0001120  ); 한영우   (전북대학교 수의과대학 미생물학교실 및 생체안전성연구소UU0001120  ); Aleyas, Abi George    (전북대학교 수의과대학 미생물학교실 및 생체안전성연구소   ); George, June Abi    (전북대학교 수의과대학 미생물학교실 및 생체안전성연구소   ); 윤현아   (전북대학교 수의과대학 미생물학교실 및 생체안전성연구소UU0001120  ); 어성국   (전북대학교 수의과대학 미생물학교실 및 생체안전성연구소UU0001120  );
  • 초록

    Background: Memory T lymphocytes of the immune system provide long-term protection in response to bacterial or viral infections/immunization. Ag concentration has also been postulated to be important in determining whether T cell differentiation favors effector versus memory cell development. In the present study we hypothesized that naive Ag-specific $CD4^+$ T cells briefly stimulated with different Ag doses at the primary exposure could affect establishment of memory cell pool after secondary immunization. Methods: To assess this hypothesis, the response kinetics of DO11.10 TCR $CD4^+$ T cells primed with different Ag doses in vitro was measured after adoptive transfer to naive BALB/c mice. Results: Maximum expansion was shown in cells primarily stimulated with high doses of ovalbumin peptide $(OVA_{323-339})$ , whereas cells in vitro stimulated with low dose were expanded slightly after in vivo secondary exposure. However, the cells primed with low $OVA_{323-339}$ peptide dose showed least contraction and established higher number of memory cells than other treated groups. When the cell division was analyzed after adoptive transfer, the high dose Ag-stimulated donor cells have undergone seven rounds of cell division at 3 days post-adoptive transfer. However, there was very few division in naive and low dose of peptide-treated group. Conclusion: These results suggest that primary stimulation with a low dose of Ag leads to better memory $CD4^+$ T cell generation after secondary immunization. Therefore, these facts imply that optimally primed $CD4^+$ T cells is necessary to support effective memory pool following administration of booster dose in prime-boost vaccination.


  • 주제어

    $CD4^+$ T cells .   primary exposure .   secondary response .   memory T cell pool.  

  • 참고문헌 (40)

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    1. 2005 "Epitope발현 DNA Vaccine과 Recombinant Vaccinia Virus를 이용한 Heterologous Prime-boost Vaccination에 의하여 유도되는 CD8+ T 세포 매개성 면역" Immune network : official journal of the Korean association of immunobiologists 5 (2): 89~98    
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