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오미자 활성성분 DDB의 NF-${\kappa}B$ 신호 전달 및 염증물질 발현 조절
Regulation of Inflammatory Repertoires and NF-${\kappa}B$ Signal Transduction by DDB, an Active Compound from Schizandra Chinensis Baillon

주성수   (중앙대학교 약학대학 면역학교실UU0001197  ); 유영민   (상지대학교 한의과대학 병리학교실UU0000668  ); 원태준   (중앙대학교 약학대학 면역학교실UU0001197  ); 김민정   (중앙대학교 약학대학 면역학교실UU0001197  ); 이선구   (상지대학교 한의과대학 병리학교실UU0000668  ); 황광우   (중앙대학교 약학대학 면역학교실UU0001197  ); 이도익   (중앙대학교 약학대학 면역학교실UU0001197  );
  • 초록

    Background: Chronic inflammation in the brain has known to be associated with the development of a various neurological diseases including dementia. In general, the characteristic of neuro-inflammation is the activated microglia over the brain where the pathogenesis occurs. Pro-inflammatory repertoires, interleukin-1 ${\beta}$ (IL-1 ${\beta}$ ) and nitric oxide (NO), are the main causes of neuro-degenerative disease, particularly in Alzheimer's disease (AD) which is caused by neuronal destruction. Those pro-inflammatory repertoires may lead the brain to chronic inflammatory status, and thus we hypothesized that chronic inflammation would be inhibited when pro-inflammatory repertoires are to be well controlled by inactivating the signal transduction associated with inflammation. Methods: In the present study, we examined whether biphenyl dimethyl dicarboxylate (DDB), an active compound from Schizandra chinensis Baillon, inhibits the NO production by a direct method using Griess reagent and by RT-PCR in the gene expression of inducible nitric oxide synthase (iNOS) and IL-1 ${\beta}$ . Western blots were also used for the analysis of NF- ${\kappa}B$ and I ${\kappa}B$ . Results: In the study, we found that DDB effectively inhibited IL-1 ${\beta}$ as well as NO production in BV-2 microglial cell, and the translocation of NF- ${\kappa}B$ was comparably inhibited in the presence of DDB comparing those to the positive control, lipopolysaccharide. Conclusion: The data suggested that the DDB from Schizandra chinensis Baillon may play an effective role in inhibiting the pro-inflammatory repertoires which may cause neurodegeneration and the results imply that the compound suppresses a cue signal of the microglial activation which can induce the brain pathogenesis such as Alzheimer's disease.


  • 주제어

    Nitric oxide .   iNOS .   NF- ${\kappa}B$ .   IL-1 ${\beta}$ .   biphenyl dimethyl dicarboxylate .   alzheimer's disease.  

  • 참고문헌 (26)

    1. Shoji K, Yasufumi O: Effect of gomicin A on liver generation. Planta Med 58;489-482, 1992 
    2. Jang EH, Pyo YH, Ahn MS: Antioxidant effect of Omija extracts. Korean J Soc Food Sci 12;372-376, 1996 
    3. Kang KW, Kim YG, Kim CW, Kim SG: The anti-fibrogenic effect of a pharmaceutical composition of [5-(2-pyrazinyl)-4-methyl- 1,2-bithiol-3-thione] (oltipraz) and dimethyl-4,4'-dime thoxy-5, 6,5',6'-dimethylene dioxybiphenyl-2,2'-dicarboxylate (DDB). Arch Pharm Res 25;655-663, 2002 
    4. Hobbs AJ, Higgs A, Moncada S: Inhibition of nitric oxide synthase as a potential therapeutic target. Annu Rev Pharmacol Toxicol 39;191-220, 1999 
    5. Kang KW, Kim YG, Kim CW, Kim SG. The anti-fibrogenic effect of a pharmaceutical composition of [5-(2-pyrazinyl)-4- methyl-1,2-dithiol-3-thione] (oltipraz) and dimethyl-4,4'-demethoxy- 5,6,5',6'-dimethylene dioxybiphenyl-2,2'-dicarboxylate (DDB). Arch Pharm Res 25;655-663, 2004 
    6. Petrova TV, Akama KT, Van Eldik LJ: Selective modulation of BV-2 microglial activation by prostaglandin E (2). Differential effects on endotoxin-stimulated cytokine induction. J Biol Chem 274;28823-288237, 1999 
    7. Furge LL, Fields PR, Goode WE, Konwinski RR, Tressler MC, Stevens-Truss R: Oltipraz inhibits inducible nitric oxide synthase in vitro and inhibits nitric oxide production in activated microglial cells. Arch. Biochem Biophys 424;163-170, 2004 
    8. Tsoulfas G, Geller DA: NF-kappaB in transplantation: friend or foe. Transpl Infect Dis 3;212-219, 2001 
    9. Min KJ, Pyo HK, Yang MS, Ji KA, Jou I, Joe EH: Gangliosides activate microglia via protein kinase C and NADPH oxidase. Glia 1548;197-206, 2004 
    10. Joo SS, Won TJ, Lee DI: Potential role of ursodeoxycholic acid in suppression of nuclear factor kappa B in microglial cell line (BV-2). Arch Pharm Res 27;954-960, 2004 
    11. Lowenstein CJ, Dinerman JL, Snyder SH: Nitric oxide: a physiologic messenger. Ann Intern Med 120;227-237, 1994 
    12. Shin HM, Kim MH, Kim BH, Jung SH, Kim YS, Park HJ, Hong JT, Min KR, Kim Y: Inhibitory action of novel aromatic diamine compound on lipopolysaccharide-induced nuclear translocation of NF-kappaB without affecting IkappaB degradation. FEBS Lett 571;50-54, 2004 
    13. Bocchini V, Mazzolla R, Barluzzi R, Blasi E, Sick P, Kettenmann H: An immortalized cell line expresses properties of activated microglial cells. J Neurosci Res 31;616-621, 1992 
    14. Kim WK, Jang PG, Woo MS, Han IO, Piao HZ, Lee K, Lee H, Joh TH, Kim HS: A new anti-inflammatory agent KL-1037 represses proinflammatory cytokine and inducible nitric oxide synthase (iNOS) gene expression in activated microglia. Neuropharmacology 47;243-252, 2004 
    15. Kim OC, Jang HJ: Volatile components of Schizandra chinenesis Bullion. Agric Chem Biotechnol 37;30-36, 1994 
    16. Meda L, Cassatella MA, Szendrei GI, Otvos LJr, Baron P, Villalba M, Ferrari D, Rossi F: Activation of microglial cells by beta-amyloid protein and interferon-gamma. Nature 374; 647-650, 1995 
    17. Kreutzberg GW: Microglia a sensor for pathological events in the CNS. Trends Neurosci 19;312-318, 1996 
    18. Selkoe DJ: Alzheimer's disease: genes, proteins, and therapy. Physiol Rev 81;741-766, 2001 
    19. Blasi E, Barluzzi R, Bocchini V, Mazzolla R, Bistoni F: Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus. J Neuroimmunol 27;229-237, 1990 
    20. Molokovskii DS, Davydov VV, Tiulenev VV: The action of adaptogenic plant preparations in experimental alloxan diabetes. Probl Endokrinol 35;82-87, 1987 
    21. Yamamoto Y, Gaynor RB: IkappaB kinases: key regulators of the NF-kappaB pathway. Trends Biochem Sci 29;72-79, 2004 
    22. Zhu M, Lin KF, Yeung RY, Li RC: Evaluation of the protective effects of Schizandra chinensis of phase I drug metabolism using a $CCl_4$ intoxication model. J Ethnopharmacol 67; 61-68, 1999 
    23. Kim HS, Whang SY, Woo MS, Park JS, Kim WK, Han IO: Sodium butyrate suppresses interferon-gamma, but not lipopolysaccharide- mediated induction of nitric oxide and tumor necrosis factor-alpha in microglia. J Neuroimmunol 151;85-93, 2004 
    24. Jung GT, Ju IO, Choi JS, Hong JS: The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis RUPRECHT (Omija) seed. Korean J Food Sci Technol 32;928-935, 2000 
    25. Kim WK, Jang PG, Woo MS, Han IO, Piao HZ, Lee K, Lee H, Joh TH, Kim HS: A new anti-inflammatory agent KL-1037 represses proinflammatory cytokine and inducible nitric oxide synthase (iNOS) gene expression in activated microglia. Neuropharmacology 47;243-52, 2004 
    26. Shoe HJ, Lee MY, Hwang GS: The effect of Scizandra fructus extract on blood constituents of alloxan-induced diabetes rabbits. J Korean Soc Food Nutr 24;658-662, 1995 

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