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약학회지 = Yakhak hoeji v.50 no.2, 2006년, pp.105 - 110  

Tributyltin 화합물이 PC12 세포의 Dopamine 생합성 저해작용에 미치는 영향
Inhibitory Effects of Tributyltin Acetate on Dopamine Biosynthesis in PC12 Cells

김유미   (충북대학교 약학대학UU0001309  ); 이재준   (충북대학교 약학대학UU0001309  ); 이명구   (충북대학교 약학대학UU0001309  );
  • 초록

    The effects of tributyltin acetate (TBTA), one of the endocrine-disrupting organotin compounds, on dopamine biosynthesis in PC12 cells were investigated. Treatment of PC12 cells with TBTA at $0.05\sim0.25{\mu}M$ significantly decreased the intracellular dopamine content in a concentration-dependent manner ( $IC_{50}$ value, $0.17{\mu}M$ ). Under these conditions, tyrosine hydroxylase (TH) activity and TH mRNA level were also decreased by $0.1{\mu}M$ TBTA at 24 h, and recovered there-after. In addition, treatment with L-DOPA at 20 and $50 {\mu}M$ increased the intracellular dopamine content in PC12 cells and the increase in dopamine content by L-DOPA was significantly abolished by TBTA at $0.1\sim0.2{\mu}M$ . These results indicate that TBTA at $0.1\sim0.2{\mu}M$ causes the decrease in the basal dopamine content and abolishes the increase in dopamine content in L-DOPA-treated cells in part by the inhibition of TH gene expression and activity.


  • 주제어

    tributyltin acetate .   dopamine content .   tyrosine hydroxylase .   PC12 cells.  

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