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동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine v.20 no.6, 2006년, pp.1530 - 1537   피인용횟수: 2
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길경 수용액 추출물에 의한 NCI-H460 인체 폐암세포의 p53 및 pRB의 발현에 미치는 영향
Effects of an Extract from the Roots of Platycodon Grandiflorum on the Levels of p53 and pRB in NCI-H460 Human Lung Carcinoma Cells

박봉규    (동의대학교 한방내과학교실   ); 감철우    (동의대학교 한방내과학교실   ); 허태율    (동의대학교 한방내과학교실   ); 박동일    (동의대학교 한방내과학교실  );
  • 초록

    Platycodi Radix, the root of Platycodon grandiflorum A. DC (Campanulaceae), commonly known as Doraji in Korea (Chinese name, 'Jiegeng', and Japanese name, 'Kikyo') has been used as an expectorant in traditional Oriental medicine. Extracts from the roots of P. grandiflorum have been reported to have wide ranging health benefits. In Korea, Platycodi Radix is also used as a food and employed as a folk remedy for adult diseases, such as bronchitis, asthma and pulmonary tuberculosis, hyperlipidemia, diabetes, and inflammatory diseases, and as a sedative. Several studies on its chemical and immunopharmacological effects including immunostimulation and antitumor activity have been performed. However, the relevant molecular mechanisms are poorly understood. Platycodi Radix, the root of Platycodon grandiflorum A. DC (Campanulaceae), commonly known as Doraji in Korea (Chinese name, 'Jiegeng', and Japanese name, 'Kikyo') has been used as an expectorant in traditional Oriental medicine. Extracts from the roots of P. grandiflorum have been reported to have wide ranging health bensfits. In Korea, Platycodi Radix is also used as a food and employed as a folk remedy for adult diseases, such as bronchitis, asthma and pulmonary tuberculosis, hyperlipidemia, diabetes, and inflammatory diseases, and as a sedative. Several studies on its chemical and immunopharmacological effects including immunostimulation and antitumor activity have been performed. However, the relevant molecular mechanisms are poorly understood. In the present study, we investigated the effects of an aqueous extract from the roots of P. grandiflorum AEPG) on the cell growth of human lung adenocarcinoma NCI-H460 cells in order to understand its anti-proliferative mechanism. AEPG treatment down-regulated the cyclin D1 expression in both transcriptional and translational levels without alteration of cyclin E. In AEPG-treated cells, the levels of cyclin-dependent kinase (C아) 6 mRNA and protein were significantly inhibited, but the levels of Cdk2 and Cdk4 were slightly inhibited by treatment of AEPG. AEPG treatment induced a marked accumulation of Cdk inhibitors, p16 and p27. However, AEPG treatment did not affect not only retinoblastoma protein (pRB) but also tumor suppressor p53 protein expression. The present results indicated that AEPG-induced inhibition of lung cancer cell proliferation is associated with the blockage of G1 phase progression through induction of Cdk inhibitors such as p16 and p27, and inhibition of cyclin D1 and Cdk6. AEPG exposure, as offered by this study, provides cluse for the mechanism of AEPG action. Taken together, these findings suggest that P. grandiflorum has strong potential for development as an agent for prevention and treatiment against human lung cancer.


  • 주제어

    길경(桔梗).  

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  • 이 논문을 인용한 문헌 (2)

    1. Choi, Eun-A ; Kim, Jung-Keun ; Kim, Kyung-Soon ; Choi, Jung-Eun ; Cho, Chong-Kwan ; Lee, Yeon-Weol ; Yoo, Hwa-Seung 2010. "Anticancer Effects of Egg White Combined-Chalcanthite on NCI-H460 Tumor Regression Model" 동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, 24(2): 272~277     
    2. Hong, Su-Hyun ; Han, Min-Ho ; Park, Cheol ; Park, Sang-Eun ; Hong, Sang-Hoon ; Choi, Yung-Hyun 2014. "Induction of Autophagy and Apoptosis by the Roots of Platycodon grandiflorum on NCI-H460 Human Non-small Lung Carcinoma Cells" 대한한방내과학회지 = The journal of internal Korean medicine, 35(3): 317~331     

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