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Streptococcus mutans의 자당 대사에 미치는 과당의 영향
THE EFFECT OF FRUCTOSE ON THE METABOLISM OF SUCROSE BY STREPTOCOCCUS MUTANS

심직현   (전남대학교 치과대학 보철학교실UU0001112  ); 방몽숙   (전남대학교 치과대학 보철학교실UU0001112  ); 양홍서   (전남대학교 치과대학 보철학교실UU0001112  ); 박상원   (전남대학교 치과대학 보철학교실UU0001112  ); 박하옥   (전남대학교 치과대학 보철학교실UU0001112  ); 오종석   (전남대학교 의과대학 미생물학교실UU0001112  ); 이재봉   (서울대학교 치과대학 보철학교실UU0000691  );
  • 초록

    Statement of problem: Streptococcus produces energy and forms extracellular polysaccharides by metabolizing sucrose. Insoluble glucan, a kind of extracellular polysaccharide, is the important material of dental plaque. Fructose affects the metabolism of sucrose. Purpose: The purpose of this study was to evaluate the effect of fructose on the metabolism of sucrose in Streptococcus mutans. Materials and methods: To determine the effect of fructose on the formation of artificial plaque by Streptococcus mutans Ingbritt, S. mutans and fructose were placed in beakers containing M17 broth and sucrose. The wires were hung on frameworks inserted into cork stoppers, and then immersed in each of the beakers. After the incubation with gentle shaking, each wire was weighed. To analyze the effect of fructose on the sucrose metabolism by S. mutans or glucosyltransferase, S. mutans and fructose were placed in M17 broth containing sucrose. After the incubation. the remaining sucrose and polymers were analysed by thin layer chromatography. Results: The following results were obtained; 1. When Streptococcus mutans was cultured in the media containing 3% sucrose for 8 hours, the mean weight of formed artificial plaque on the wires was $124.3{\pm}3.0mg$ , whereas being reduced to $20.7{\pm}10.2mg$ in the media added with 3% sucrose and 4% fructose(p


  • 주제어

    Streptococcus mutans .   Sucrose .   Fructose .   Glucan.  

  • 참고문헌 (19)

    1. McDougall WA. Studies on the dental plaque. I. The histology of the dental plaque and its attachment. Aust Dent J 1963:8:261-273 
    2. Dewar MG. Walker GJ. Metabolism of the polysaccharides of human dental plaque. Caries Res 1975:9:21-35 
    3. Toda Y. Moro I. Koga T. Asakawa H. Hamada S. Ultrastruture of extracellular polysaccharides produced by serotype c Streptococcus mutans. J Dent Res 1987: 66:1364-1369 
    4. Tanzer JM. Microbiology of dental caries. In: Contemporary oral microbiology and immunology. edited by Slots J. and Taubman M. St. Louis: Mosby, pp. 1992 p.377-424 
    5. Rolla G. Scheie AA Ciardi JE. Role of sucrose in plaque formation. Scand. J Dent Res 1985: 93: 105-111 
    6. Halhoul MN, Kleinberg I. Differential determination of glucose and fructose, and glucose- and fructose-yielding substances with anthrone. Ana Biochem 1972;50:337-343 
    7. Hammond BF. Dextran production by a human oral strain of Lactobacillus casei. Archs Oral Biol 1969: 14:879-890 
    8. Hamada S. Slade HD. Biology. immunology and cariogenicity of Streptococcus mutans. Microbiol Rev 1980:44:331-384 
    9. Gibbons RJ. van Houte J. Bacterial adherence in oral microbial ecology. Ann Rev Microbiol 1975: 29: 19-44 
    10. Nakano YJ, Kuramitsu HK. Mechanism of Streptococcus mutans glucosyltransferase: hybrid-enzyme analysis. J Bacteriol 1992: 174:5639-5646 
    11. Clarke JK. On the bacterial factor in the aetiology of dental caries. Br J Exp Pathol 1924;5:141-147 
    12. Guggenheim B. Extracellular polysaccharides and microbial plaque. Int Dent J 1970:20:657-678 
    13. Gibbons RJ. Banghart SS. Synthesis of extracellular dextran by cariogenic bacteria and its presence in human dental plaque. Arch Oral Biol 1967: 12: 11-24 
    14. Aoki H. Shiroza T, Hayakawa M, Sato S, Kuramitsu HK. Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis. Infect Immun 1986:53: 587-594 
    15. Park KC. Tooth plaque (2). Dental research 1998:43:23-30 
    16. Hanada N, Kuramitsu HK. Isolation and characterization of the Streptococcus mutans gtfC gene, coding for primer-dependent soluble glucan synthesis. Infect Immun 1989:57:2079-2085 
    17. Hanada N, Kuramitsu HK. Isolation and characterization of the Streptococcus rnutans gtfC gene, coding for synthesis of both soluble and insoluble glucans. Infect Immun 1988:56: 1999-2005 
    18. Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986; 50: 353-380 
    19. Newbrun E. Polysaccharide synthesis in plaque. Microbiology Abstract Suppl. Microbial aspect of dental caries III. 1979 p.659 

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  • 심직현 (1)

    1. 1995 "지대치 코어 재료와 In-Ceram coping의 두께가 In-Ceram의 색에 미치는 영향" 대한치과보철학회지 = The journal of Korean academy of prosthodontics 33 (4): 634~644    
  • 방몽숙 (72)

  • Yang, Hong-So (89)

  • Park, Sang-Won (76)

  • Park, Ha-Ok (19)

  • 오종석 (45)

  • 이재봉 (62)

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