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Beta hemolysis 유발 병원균 Bacillus cereus의 HQNO-sensitive NADH:DCIP oxidoreductase
HQNO-sensitive NADH:DCIP Oxidoreductase of a Pathogenic Bacillus cereus Causing β-Hemolysis

김영재    (창원대학교 자연과학대학 미생물학과   ); 박기태    (박기태 한의원  );
  • 초록

    호기적으로 자란 Bacillus cereus KCTC 3674로 부터 조제된 막은 NADH만을 산화하고, deamino-NADH는 거의 산화하지 않았다. 호흡쇄와 연계된 NADH oxidase계는 $K_m$ 값이 약 $65\;{\mu}M$ 이였다. NADH:DCIP oxidoreductase의 활성은 $Na^+$ 또는 $K^+$ 에 의해 감소되었다. 그 최적 pH는 5.5 였다. NADH:DCIP oxidoreductase의 활성은 rotenone, capsaicin, $AgNO_3$ 와 같은 호흡저해제에는 매우 저항적 이 였지만, $40{\mu}M$ HQNO (2-heptyl-4-hydroxyquinoline-N-oxide) 존재하에서는 약 40% 저해되었다. 이들 결과로 부터, Bacillus cereus KCTC 3674의 호기적 호흡쇄와 연계된 NADH oxidase계는 energy coupling site가 결여된 HQNO-sensitive NADH:DCIP oxidoreductase를 소유하고 있는 것으로 추정된다.


    Membranes prepared from Bacillus cereus KCTC 3674, grown aerobically on a complex medium, oxidized NADH exclusively, whereas deamino-NADH was little oxidized. The respiratory chain-linkedNADH oxidase system exhibited an apparent $K_m$ value of about $65\;{\mu}M$ for NADH. Interestingly, the activity of NADH:DCIP oxidoreductase on NADH oxidase system was decreased remarkably by $Na^+$ or $K^+$ , and its optimal pH was 5.5. The activity of NADH:DCIP oxidoreductase was very resistant to the respiratory chain inhibitors such as rotenone, capsaicin, and $AgNO_3$ , whereas it was inhibited by about 40% with $40{\mu}M$ 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO). From the results, we suggest the possibility that the aerobic respiratory chain-linked NADH oxidase system of B. cereus KCTC 3674 may possess the HQNO-sensitive NADH:DCIP oxidoreductase lacking an energy coupling site.


  • 주제어

    Bacillus cereus KCTC 3674 .   aerobic respiratory chain .   NADH:DCIP oxidoreductase.  

  • 참고문헌 (14)

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 저자의 다른 논문

  • Kim, Young-Jae (14)

    1. 1996 "$Na^{+}$-dependent NADH:quinone Oxidoreductase in the Respiratory Chain of the Marine Bacterium Marinomonas vaga" Journal of microbiology and biotechnology 6 (6): 391~396    
    2. 1998 "Taxonomic Studies of the Beta Hemolysis-causing Pathogen Bacillus cereus Isolated from Sea Water" Journal of microbiology and biotechnology 8 (1): 67~73    
    3. 1999 "해양 호염성 세균 Vibrio alginolyticus가 생산하는 Extracellular Amylase의 특성" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 27 (3): 203~207    
    4. 2000 "The Succinate : Quinone Oxidoreductase of Marine Bacterium Vibiro alginolyticus is a $H^+$" Journal of microbiology and biotechnology 10 (1): 48~50    
    5. 2000 "Influence of Temperature, Oxygen, m-Chlorophenylhydrazone Cerulenin, and Quinacrine on the Production of Extracellular Proteases in Bacillus cereus" Journal of microbiology and biotechnology 10 (1): 103~106    
    6. 2001 "Thin Layer Chromatogram by an Extracellular ${\beta}$-Amylase of Bacillus sp. KYJ 963 and its Amino Acid Composition" Journal of life science 11 (2): 92~93    
    7. 2003 "The Membrane-Bound NADH:Ubiquinone Oxidoreductase in the Aerobic Respiratory Chain of Marine Bacterium Pseudomonas nautica" Journal of microbiology and biotechnology 13 (2): 225~229    
    8. 2004 "자생식물 추출물의 세포 분산 활성" 생약학회지 35 (1): 62~79    
    9. 2004 "Enzymatic Properties of the Membrane-bound NADH Oxidase System in the Aerobic Respiratory Chain of Bacillus cereus" Journal of biochemistry and molecular biology = 한국생화학회지 37 (6): 753~756    
    10. 2004 "The Influence of NaCl and Carbonylcyanide-m-Chlorophenylhydrazone on the Production of Extracellular Proteases in a Marine Vibrio Strain" The journal of microbiology 42 (2): 156~159    

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